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UniProtKB/Swiss-Prot entry Q96P70


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name IPO9_HUMAN
Primary accession number Q96P70
Secondary accession numbers Q8N1Y1 Q8N3I2 Q8NCG9 Q96SU6 Q9NW01 Q9P0A8 Q9ULM8
Integrated into Swiss-Prot on February 2, 2004
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    June 16, 2009 (Entry version 67)
Name and origin of the protein
Protein name Importin-9
Synonyms Imp9
Ran-binding protein 9
RanbP9
Gene name
Name: IPO9
Synonyms: IMP9, KIAA1192, RANBP9
ORFNames: HSPC273
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INTERACTION WITH RPS7; RPL18A; RPL4 AND RPL6.
DOI=10.1093/emboj/21.3.377; PubMed=11823430 [NCBI, ExPASy, EBI, Israel, Japan]
Jaekel S., Mingot J.-M., Schwarzmaier P., Hartmann E., Goerlich D.;
"Importins fulfil a dual function as nuclear import receptors and cytoplasmic chaperones for exposed basic domains.";
EMBO J. 21:377-386(2002).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-276 AND 358-1041.
TISSUE=Amygdala;
DOI=10.1038/ng1285; PubMed=14702039 [NCBI, ExPASy, EBI, Israel, Japan]
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
"Complete sequencing and characterization of 21,243 full-length human cDNAs.";
Nat. Genet. 36:40-45(2004).
[3]
PROTEIN SEQUENCE OF 2-22; 400-427 AND 908-916, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, AND MASS SPECTROMETRY.
TISSUE=B-cell lymphoma;
Bienvenut W.V.;
Submitted (JUN-2005) to UniProtKB.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 406-1041.
TISSUE=Placenta;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 632-1041.
TISSUE=Amygdala;
DOI=10.1186/1471-2164-8-399; PubMed=17974005 [NCBI, ExPASy, EBI, Israel, Japan]
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I.;
"The full-ORF clone resource of the German cDNA consortium.";
BMC Genomics 8:399-399(2007).
[6]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 767-1041.
TISSUE=Umbilical cord blood;
DOI=10.1101/gr.140200; PubMed=11042152 [NCBI, ExPASy, EBI, Israel, Japan]
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X., Gu J., Chen S.-J., Chen Z.;
"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells.";
Genome Res. 10:1546-1560(2000).
[7]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 780-1041.
TISSUE=Brain;
DOI=10.1093/dnares/6.5.329; PubMed=10574461 [NCBI, ExPASy, EBI, Israel, Japan]
Hirosawa M., Nagase T., Ishikawa K., Kikuno R., Nomura N., Ohara O.;
"Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain.";
DNA Res. 6:329-336(1999).
[8]
INTERACTION WITH PPP2R1A AND PPP2R1B.
DOI=10.1016/S0006-291X(03)00434-0; PubMed=12670497 [NCBI, ExPASy, EBI, Israel, Japan]
Lubert E.J., Sarge K.D.;
"Interaction between protein phosphatase 2A and members of the importin beta superfamily.";
Biochem. Biophys. Res. Commun. 303:908-913(2003).
[9]
IDENTIFICATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
Colinge J., Superti-Furga G., Bennett K.L.;
Submitted (OCT-2008) to UniProtKB.
Comments
  • FUNCTION: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6.
  • SUBUNIT: Binds with high affinity to RPS7 and RPL18A. The binding is coupled to RanGTP cycles. May bind H2A, H3, H4 histones (By similarity), RPL4 and RPL6 with low affinity. Interacts with PPP2R1A and PPP2R1B.
  • SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity).
  • SIMILARITY: Belongs to the importin beta family.
  • SIMILARITY: Contains 1 importin N-terminal domain.
  • SEQUENCE CAUTION:
    • Sequence=AAF28951.1; Type=Frameshift; Positions=982;
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF410465; AAL01416.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK001264; BAA91588.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK027532; BAB55181.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK074740; BAC11173.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK094603; BAC04383.1; ALT_SEQ; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC003604; AAH03604.2; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF161391; AAF28951.1; ALT_FRAME; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL834323; CAD38991.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB033018; BAA86506.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00185146; -.
RefSeq NP_060555.2; -.
UniGene Hs.596014
3D structure databases
ModBase Q96P70.
Protein-protein interaction databases
IntAct Q96P70; 5.
PTM databases
PhosphoSite Q96P70; -.
Organism-specific databases
GeneCards GC01P200064; -.
H-InvDB HIX0001464; -.
HGNC HGNC:19425; IPO9.
GenAtlas IPO9.
PharmGKB PA134930111; -.
HUGE KIAA1192.
Gene expression databases
ArrayExpress Q96P70; -.
Bgee Q96P70; -.
CleanEx HS_IPO9; -.
HS_RANBP9; -.
GermOnline ENSG00000198700; Homo sapiens.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from direct assay from UniProtKB).
GO:0005643; Cellular component: nuclear pore (inferred from electronic annotation from InterPro).
GO:0042393; Molecular function: histone binding (inferred from sequence or structural similarity from UniProtKB).
GO:0008565; Molecular function: protein transporter activity (inferred from direct assay from UniProtKB).
GO:0008536; Molecular function: Ran GTPase binding (inferred from sequence or structural similarity from UniProtKB).
GO:0000059; Biological process: protein import into nucleus, docking (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR011989; ARM-like.
IPR001494; Importin-b_N.
Graphical view of domain structure.
Gene3D G3DSA:1.25.10.10; ARM-like; 1.
Pfam PF03810; IBN_N; 1.
Pfam graphical view of domain structure.
PROSITE PS50166; IMPORTIN_B_NT; 1.
PROSITE graphical view of domain structure (profiles).
Proteomic databases
PeptideAtlas Q96P70; -.
PRIDE Q96P70; -.
Genome annotation databases
Ensembl ENSG00000198700; Homo sapiens. [Contig view]
GeneID 55705; -.
KEGG hsa:55705; -.
Phylogenomic databases
HOVERGEN Q96P70; -.
OMA Q96P70; ALQMPDG.
Other
NextBio 60559; -.
ProtoNet Q96P70.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Acetylation; Cytoplasm; Direct protein sequencing; Nucleus; Protein transport; Transport.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1      1        Removed. 
CHAIN   2   1041  1040     Importin-9. PRO_0000120754
DOMAIN   43    119  77     Importin N-terminal. 
MOD_RES   2      2        N-acetylalanine. 
CONFLICT   632    632        E -> G (in Ref. 2; BAC11173). 
CONFLICT   916    916        K -> R (in Ref. 2; BAC11173). 
CONFLICT   935    935        R -> P (in Ref. 2; BAB55181). 
CONFLICT   960    960        E -> G (in Ref. 2; BAC11173). 
Sequence information
Length: 1041 AA [This is the length of the unprocessed precursor] Molecular weight: 115963 Da [This is the MW of the unprocessed precursor] CRC64: A1842C357AEDFD90 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAAAAAAGAA SGLPGPVAQG LKEALVDTLT GILSPVQEVR AAAEEQIKVL EVTEEFGVHL 

        70         80         90        100        110        120 
AELTVDPQGA LAIRQLASVI LKQYVETHWC AQSEKFRPPE TTERAKIVIR ELLPNGLRES 

       130        140        150        160        170        180 
ISKVRSSVAY AVSAIAHWDW PEAWPQLFNL LMEMLVSGDL NAVHGAMRVL TEFTREVTDT 

       190        200        210        220        230        240 
QMPLVAPVIL PEMYKIFTMA EVYGIRTRSR AVEIFTTCAH MICNMEELEK GAAKVLIFPV 

       250        260        270        280        290        300 
VQQFTEAFVQ ALQIPDGPTS DSGFKMEVLK AVTALVKNFP KHMVSSMQQI LPIVWNTLTE 

       310        320        330        340        350        360 
SAAFYVRTEV NYTEEVEDPV DSDGEVLGFE NLVFSIFEFV HALLENSKFK STVKKALPEL 

       370        380        390        400        410        420 
IYYIILYMQI TEEQIKVWTA NPQQFVEDED DDTFSYTVRI AAQDLLLAVA TDFQNESAAA 

       430        440        450        460        470        480 
LAAAATRHLQ EAEQTKNSGT EHWWKIHEAC MLALGSVKAI ITDSVKNGRI HFDMHGFLTN 

       490        500        510        520        530        540 
VILADLNLSV SPFLLGRALW AASRFTVAMS PELIQQFLQA TVSGLHETQP PSVRISAVRA 

       550        560        570        580        590        600 
IWGYCDQLKV SESTHVLQPF LPSILDGLIH LAAQFSSEVL NLVMETLCIV CTVDPEFTAS 

       610        620        630        640        650        660 
MESKICPFTI AIFLKYSNDP VVASLAQDIF KELSQIEACQ GPMQMRLIPT LVSIMQAPAD 

       670        680        690        700        710        720 
KIPAGLCATA IDILTTVVRN TKPPLSQLLI CQAFPAVAQC TLHTDDNATM QNGGECLRAY 

       730        740        750        760        770        780 
VSVTLEQVAQ WHDEQGHNGL WYVMQVVSQL LDPRTSEFTA AFVGRLVSTL ISKAGRELGE 

       790        800        810        820        830        840 
NLDQILRAIL SKMQQAETLS VMQSLIMVFA HLVHTQLEPL LEFLCSLPGP TGKPALEFVM 

       850        860        870        880        890        900 
AEWTSRQHLF YGQYEGKVSS VALCKLLQHG INADDKRLQD IRVKGEEIYS MDEGIRTRSK 

       910        920        930        940        950        960 
SAKNPERWTN IPLLVKILKL IINELSNVME ANAARQATPA EWSQDDSNDM WEDQEEEEEE 

       970        980        990       1000       1010       1020 
EEDGLAGQLL SDILATSKYE EDYYEDDEED DPDALKDPLY QIDLQAYLTD FLCQFAQQPC 

      1030       1040 
YIMFSGHLND NERRVLQTIG I 

Q96P70 in FASTA format

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