ID   EDC3_HUMAN              Reviewed;         508 AA.
AC   Q96F86; Q9H797;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   14-OCT-2008, entry version 52.
DE   RecName: Full=Enhancer of mRNA-decapping protein 3;
DE   AltName: Full=LSM16 homolog;
DE   AltName: Full=YjeF domain-containing protein 1;
DE   AltName: Full=YjeF N-terminal domain-containing protein 2;
DE            Short=YjeF_N2;
DE            Short=hYjeF_N2;
GN   Name=EDC3; Synonyms=LSM16, YJDC, YJEFN2; ORFNames=PP844;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15498874; DOI=10.1073/pnas.0404089101;
RA   Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H.,
RA   Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y.,
RA   Shu H., Chen X., Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S.,
RA   Gu J.;
RT   "Large-scale cDNA transfection screening for genes related to cancer
RT   development and progression.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Eye, and Placenta;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH DCP1A; DCP2;
RP   DDX6; EDC4 AND ZFP36, SUBCELLULAR LOCATION, AND FUNCTION.
RX   PubMed=16364915; DOI=10.1016/j.molcel.2005.10.031;
RA   Fenger-Groen M., Fillman C., Norrild B., Lykke-Andersen J.;
RT   "Multiple processing body factors and the ARE binding protein TTP
RT   activate mRNA decapping.";
RL   Mol. Cell 20:905-915(2005).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Epithelium;
RX   PubMed=16964243; DOI=10.1038/nbt1240;
RA   Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.;
RT   "A probability-based approach for high-throughput protein
RT   phosphorylation analysis and site localization.";
RL   Nat. Biotechnol. 24:1285-1292(2006).
RN   [6]
RP   FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=17533573; DOI=10.1055/s-2007-977699;
RA   Rudolph C., Sigruener A., Hartmann A., Orso E., Bals-Pratsch M.,
RA   Gronwald W., Seifert B., Kalbitzer H.R., Verdorfer I., Luetjens C.M.,
RA   Ortmann O., Bornstein S.R., Schmitz G.;
RT   "ApoA-I-binding protein (AI-BP) and its homologues hYjeF_N2 and
RT   hYjeF_N3 comprise the YjeF_N domain protein family in humans with a
RT   role in spermiogenesis and oogenesis.";
RL   Horm. Metab. Res. 39:322-335(2007).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131, AND MASS
RP   SPECTROMETRY.
RX   PubMed=17287340; DOI=10.1073/pnas.0611217104;
RA   Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A.;
RT   "Global proteomic profiling of phosphopeptides using electron transfer
RT   dissociation tandem mass spectrometry.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-161, AND MASS
RP   SPECTROMETRY.
RX   PubMed=18220336; DOI=10.1021/pr0705441;
RA   Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,
RA   Yates J.R. III;
RT   "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for
RT   efficient phosphoproteomic analysis.";
RL   J. Proteome Res. 7:1346-1351(2008).
RN   [9]
RP   X-RAY CRYSTALLOGRAPHY (1.31 ANGSTROMS) OF 1-82.
RX   PubMed=17923697; DOI=10.1128/MCB.01506-07;
RA   Tritschler F., Eulalio A., Truffault V., Hartmann M.D., Helms S.,
RA   Schmidt S., Coles M., Izaurralde E., Weichenrieder O.;
RT   "A divergent Sm fold in EDC3 proteins mediates DCP1 binding and P-body
RT   targeting.";
RL   Mol. Cell. Biol. 27:8600-8611(2007).
CC   -!- FUNCTION: In the process of mRNA degradation, may play a role in
CC       mRNA decapping. May play a role in spermiogenesis and oogenesis.
CC   -!- SUBUNIT: Forms a complex with DCP1A, DCP2, DDX6 and EDC4/HEDLS,
CC       within this complex directly interacts with DCP1A and DDX6.
CC       Interacts with ZFP36.
CC   -!- INTERACTION:
CC       P26196:DDX6; NbExp=1; IntAct=EBI-997311, EBI-351257;
CC       P31946:YWHAB; NbExp=1; IntAct=EBI-997311, EBI-359815;
CC       P61981:YWHAG; NbExp=1; IntAct=EBI-997311, EBI-359832;
CC       P27348:YWHAQ; NbExp=1; IntAct=EBI-997311, EBI-359854;
CC       P63104:YWHAZ; NbExp=1; IntAct=EBI-997311, EBI-347088;
CC       P26651:ZFP36; NbExp=1; IntAct=EBI-997311, EBI-374248;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, P-body. Note=Processing bodies
CC       (PB).
CC   -!- TISSUE SPECIFICITY: Expressed in theca and granulosa cells in
CC       ovary, and in spermatids of the meiotic division part II and
CC       apical membrane of Sertoli cells in testis (at protein level).
CC       Also expressed in brain and mammary gland.
CC   -!- SIMILARITY: Belongs to the EDC3 family.
CC   -!- SIMILARITY: Contains 1 YjeF N-terminal domain.
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DR   EMBL; AF193058; AAG22486.1; -; mRNA.
DR   EMBL; AK024781; BAB15001.1; -; mRNA.
DR   EMBL; BC011534; AAH11534.1; -; mRNA.
DR   EMBL; BC021271; AAH21271.1; -; mRNA.
DR   RefSeq; NP_079359.2; -.
DR   UniGene; Hs.96852; -.
DR   PDB; 2VC8; X-ray; 1.31 A; A=1-82.
DR   PDBsum; 2VC8; -.
DR   IntAct; Q96F86; -.
DR   PhosphoSite; Q96F86; -.
DR   PeptideAtlas; Q96F86; -.
DR   Ensembl; ENSG00000179151; Homo sapiens.
DR   GeneID; 80153; -.
DR   KEGG; hsa:80153; -.
DR   H-InvDB; HIX0020864; -.
DR   HGNC; HGNC:26114; EDC3.
DR   MIM; 609842; gene.
DR   HOGENOM; Q96F86; -.
DR   HOVERGEN; Q96F86; -.
DR   ArrayExpress; Q96F86; -.
DR   CleanEx; HS_EDC3; -.
DR   GermOnline; ENSG00000179151; Homo sapiens.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   InterPro; IPR004443; YjeF_N.
DR   Pfam; PF03853; YjeF_N; 1.
DR   PROSITE; PS51385; YJEF_N; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Phosphoprotein.
FT   CHAIN         1    508       Enhancer of mRNA-decapping protein 3.
FT                                /FTId=PRO_0000119054.
FT   DOMAIN      283    487       YjeF N-terminal.
FT   REGION        1     79       Required for P-body targeting and
FT                                interaction with DCP1A (By similarity).
FT   REGION      191    296       Required for interaction with DDX6 (By
FT                                similarity).
FT   MOD_RES     131    131       Phosphoserine.
FT   MOD_RES     161    161       Phosphoserine.
FT   CONFLICT     74     74       Q -> R (in Ref. 2; BAB15001).
FT   TURN          4      7
FT   STRAND        9     13
FT   TURN         16     18
FT   STRAND       20     29
FT   TURN         30     33
FT   STRAND       34     42
FT   STRAND       48     55
FT   HELIX        56     58
FT   STRAND       60     66
SQ   SEQUENCE   508 AA;  56078 MW;  95346F484FCFC3EA CRC64;
     MATDWLGSIV SINCGDSLGV YQGRVSAVDQ VSQTISLTRP FHNGVKCLVP EVTFRAGDIT
     ELKILEIPGP GDNQHFGDLH QTELGPSGAG CQVGINQNGT GKFVKKPASS SSAPQNIPKR
     TDVKSQDVAV SPQQQQCSKS YVDRHMESLS QSKSFRRRHN SWSSSSRHPN QATPKKSGLK
     NGQMKNKDDE CFGDDIEEIP DTDFDFEGNL ALFDKAAVFE EIDTYERRSG TRSRGIPNER
     PTRYRHDENI LESEPIVYRR IIVPHNVSKE FCTDSGLVVP SISYELHKKL LSVAEKHGLT
     LERRLEMTGV CASQMALTLL GGPNRLNPKN VHQRPTVALL CGPHVKGAQG ISCGRHLANH
     DVQVILFLPN FVKMLESITN ELSLFSKTQG QQVSSLKDLP TSPVDLVINC LDCPENVFLR
     DQPWYKAAVA WANQNRAPVL SIDPPVHEVE QGIDAKWSLA LGLPLPLGEH AGRIYLCDIG
     IPQQVFQEVG INYHSPFGCK FVIPLHSA
//