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UniProtKB/Swiss-Prot entry Q96301


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name SPY_ARATH
Primary accession number Q96301
Secondary accession number Q56YX8
Integrated into Swiss-Prot on November 22, 2005
Sequence was last modified on February 1, 1997 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 56)
Name and origin of the protein
Protein name Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY
Synonym EC 2.4.1.-
Gene name
Name: SPY
OrderedLocusNames: At3g11540
ORFNames: F24K9.29
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND MUTAGENESIS OF GLY-593 AND CYS-845.
STRAIN=cv. Columbia;
DOI=10.1073/pnas.93.17.9292; PubMed=8799194 [NCBI, ExPASy, EBI, Israel, Japan]
Jacobsen S.E., Binkowski K.A., Olszewski N.E.;
"SPINDLY, a tetratricopeptide repeat protein involved in gibberellin signal transduction in Arabidopsis.";
Proc. Natl. Acad. Sci. U.S.A. 93:9292-9296(1996).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/35048706; PubMed=11130713 [NCBI, ExPASy, EBI, Israel, Japan]
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
Nature 408:820-822(2000).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K., Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.;
"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
[4]
ENZYME ACTIVITY IN VITRO.
Thornton T.M., Kreppel L., Hart G.W., Olszewski N.E.;
"Genetic and biochemical analysis of Arabidopsis SPY.";
(In) Altman A., Ziv M., Izhar S. (eds.); Plant biotechnology and in vitro biology in the 21st century, pp.445-448, Kluwer Academic Publishers, New York (1999).
[5]
FUNCTION.
DOI=10.1104/pp.126.3.1174; PubMed=11457967 [NCBI, ExPASy, EBI, Israel, Japan]
Swain S.M., Tseng T.-S., Olszewski N.E.;
"Altered expression of SPINDLY affects gibberellin response and plant development.";
Plant Physiol. 126:1174-1185(2001).
[6]
HOMODIMERIZATION, AND DOMAIN.
DOI=10.1104/pp.126.3.1250; PubMed=11457975 [NCBI, ExPASy, EBI, Israel, Japan]
Tseng T.-S., Swain S.M., Olszewski N.E.;
"Ectopic expression of the tetratricopeptide repeat domain of SPINDLY causes defects in gibberellin response.";
Plant Physiol. 126:1250-1258(2001).
[7]
SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
DOI=10.1104/pp.020002; PubMed=12068105 [NCBI, ExPASy, EBI, Israel, Japan]
Swain S.M., Tseng T.-S., Thornton T.M., Gopalraj M., Olszewski N.E.;
"SPINDLY is a nuclear-localized repressor of gibberellin signal transduction expressed throughout the plant.";
Plant Physiol. 129:605-615(2002).
[8]
FUNCTION.
PubMed=12136030 [NCBI, ExPASy, EBI, Israel, Japan]
Hartweck L.M., Scott C.L., Olszewski N.E.;
"Two O-linked N-acetylglucosamine transferase genes of Arabidopsis thaliana L. Heynh. have overlapping functions necessary for gamete and seed development.";
Genetics 161:1279-1291(2002).
[9]
FUNCTION, AND INTERACTION WITH GI.
DOI=10.1105/tpc.019224; PubMed=15155885 [NCBI, ExPASy, EBI, Israel, Japan]
Tseng T.-S., Salome P.A., McClung C.R., Olszewski N.E.;
"SPINDLY and GIGANTEA interact and act in Arabidopsis thaliana pathways involved in light responses, flowering, and rhythms in cotyledon movements.";
Plant Cell 16:1550-1563(2004).
[10]
FUNCTION.
DOI=10.1105/tpc.104.028472; PubMed=15608330 [NCBI, ExPASy, EBI, Israel, Japan]
Greenboim-Wainberg Y., Maymon I., Borochov R., Alvarez J., Olszewski N., Ori N., Eshed Y., Weiss D.;
"Cross talk between gibberellin and cytokinin: the Arabidopsis GA response inhibitor SPINDLY plays a positive role in cytokinin signaling.";
Plant Cell 17:92-102(2005).
Comments
  • FUNCTION: Probable O-linked N-acetylglucosamine transferase (OGT) involved in various processes such as gibberellin (GA) signaling pathway and circadian clock. OGTs catalyze the addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Probably acts by adding O-linked sugars to yet unknown proteins. Acts as a repressor of GA signaling pathway to inhibit hypocotyl elongation. Functions with GIGANTEA (GI) in pathways controlling flowering, circadian cotyledon movements and hypocotyl elongation. Acts as a light-regulated promoter of elongation via its interaction with GI. Acts as an activator of cytokinin signaling. Required with SEC for gamete and seed development. Its OGT activity has been proved in vitro but not in vivo.
  • CATALYTIC ACTIVITY: UDP-N-acetyl-D-glucosamine + peptide = UDP + N-acetyl-beta-D-glucosaminyl-peptide.
  • PATHWAY: Protein modification; protein glycosylation.
  • SUBUNIT: Homomultimer; via its TPR repeats. Interacts with GI.
  • INTERACTION:
    Q9SQI2:GI; NbExp=1; IntAct=EBI-446372, EBI-446380;
  • SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
  • ALTERNATIVE PRODUCTS: 1 named isoform [FASTA] produced by alternative splicing. A number of isoforms are produced. According to EST sequences.
    Name1
    Isoform IDQ96301-1
    This is the isoform sequence displayed in this entry.
  • TISSUE SPECIFICITY: Widely expressed. Present throughout the plant (at protein level).
  • DEVELOPMENTAL STAGE: Detected in all organs of the plant and at all stages of the life cycle. Detected 1 day after germination in the radicle just before its emergence from the seed. At 2.5 and 3 days after germination, expression in the young seedling is highest in the cotyledons and the root tip. At 3, 4 and 5 days, expression is also detectable in the hypocotyl. At 10 days of age, expression in the first pair of true leaves is reduced relative to the rest of the seedling. 2 days later, this difference disappears and the expression level is again fairly similar throughout the aboveground portion of the plant, with a higher intensity in the vegetative apex. This developmental regulation is not detected in leaves developing at later nodes. Older plants also display uniform expression throughout the vegetative organs, but this expression is less intense. In older seedlings, expression is observed throughout the root, particularly at the tip of the primary root and in lateral roots. Expression is also observed in trichomes and senescing leaves, and in inflorescence internodes, flowers. Expression is observed in the seeds and carpels of fully elongated siliques. Lower expression is also observed in expanding siliques and in the developing seeds in these siliques. Expression is also detected in the embryo of maturing seeds (after the disappearance of the endosperm) (at protein level).
  • DOMAIN: The TPR repeats mediate protein-protein interactions and are essential for its function. Expression of such repeats in plants accelerate flowering.
  • SIMILARITY: Belongs to the O-GlcNAc transferase family.
  • SIMILARITY: Contains 11 TPR repeats.
  • SEQUENCE CAUTION:
    • Sequence=BAD94413.1; Type=Frameshift; Positions=528;
    • Sequence=BAD95289.1; Type=Frameshift; Positions=528;
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U62135; AAC49446.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC008153; AAG51433.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK220931; BAD94413.1; ALT_FRAME; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK221192; BAD95289.1; ALT_FRAME; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK221314; BAD94086.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_187761.1; -.
UniGene At.17656
3D structure databases
ModBase Q96301.
Protein-protein interaction databases
IntAct Q96301; -.
Organism-specific databases
GeneFarm 5156; -.
TAIR At3g11540; -.
Ontologies
GO
GO:0005634; Cellular component: nucleus (traceable author statement from TAIR).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0009740; Biological process: gibberellic acid mediated signaling (traceable author statement from TAIR).
QuickGo view.
Family and domain databases
InterPro IPR006597; Sel1_like.
IPR001440; TPR-1.
IPR011990; TPR-like_helical.
IPR013026; TPR_region.
Graphical view of domain structure.
Gene3D G3DSA:1.25.40.10; TPR-like_helical; 2.
Pfam PF00515; TPR_1; 10.
Pfam graphical view of domain structure.
SMART SM00671; SEL1; 4.
SM00028; TPR; 10.
SMART graphical view of domain structure.
PROSITE PS50005; TPR; 11.
PS50293; TPR_REGION; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q96301.
Proteomic databases
ProMEX Q96301; -.
Genome annotation databases
GeneID 820327; -.
GenomeReviews BA000014_GR; AT3G11540.
KEGG ath:AT3G11540; -.
NMPDR fig|3702.1.peg.13207; -.
Other
ProtoNet Q96301.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Biological rhythms; Complete proteome; Cytoplasm; Developmental protein; Differentiation; Flowering; Gibberellin signaling pathway; Glycosyltransferase; Nucleus; Repeat; TPR repeat; Transferase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   914  914     Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY. PRO_0000191776
REPEAT   43    76  34     TPR 1. 
REPEAT   77   110  34     TPR 2. 
REPEAT   112   144  33     TPR 3. 
REPEAT   152   185  34     TPR 4. 
REPEAT   186   219  34     TPR 5. 
REPEAT   220   253  34     TPR 6. 
REPEAT   261   294  34     TPR 7. 
REPEAT   295   328  34     TPR 8. 
REPEAT   329   362  34     TPR 9. 
REPEAT   364   396  33     TPR 10. 
REPEAT   397   430  34     TPR 11. 
REGION   431   914  484     Catalytic region. 
MUTAGEN   593   593        G->S: In spy-3; induces a constitutive induction of GA signal. 
MUTAGEN   845   845        C->Y: In spy-5; induces a constitutive induction of GA signal. 
Sequence information
Length: 914 AA [This is the length of the unprocessed precursor] Molecular weight: 101430 Da [This is the MW of the unprocessed precursor] CRC64: A881D84BB5C33493 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MVGLEDDTER ERSPVVENGF SNGSRSSSSS AGVLSPSRKV TQGNDTLSYA NILRARNKFA 

        70         80         90        100        110        120 
DALALYEAML EKDSKNVEAH IGKGICLQTQ NKGNLAFDCF SEAIRLDPHN ACALTHCGIL 

       130        140        150        160        170        180 
HKEEGRLVEA AESYQKALMA DASYKPAAEC LAIVLTDLGT SLKLAGNTQE GIQKYYEALK 

       190        200        210        220        230        240 
IDPHYAPAYY NLGVVYSEMM QYDNALSCYE KAALERPMYA EAYCNMGVIY KNRGDLEMAI 

       250        260        270        280        290        300 
TCYERCLAVS PNFEIAKNNM AIALTDLGTK VKLEGDVTQG VAYYKKALYY NWHYADAMYN 

       310        320        330        340        350        360 
LGVAYGEMLK FDMAIVFYEL AFHFNPHCAE ACNNLGVLYK DRDNLDKAVE CYQMALSIKP 

       370        380        390        400        410        420 
NFAQSLNNLG VVYTVQGKMD AAASMIEKAI LANPTYAEAF NNLGVLYRDA GNITMAIDAY 

       430        440        450        460        470        480 
EECLKIDPDS RNAGQNRLLA MNYINEGLDD KLFEAHRDWG WRFTRLHPQY TSWDNLKDPE 

       490        500        510        520        530        540 
RPITIGYISP DFFTHSVSYF IEAPLTHHDY TKYKVVVYSA VVKADAKTYR FRDKVLKKGG 

       550        560        570        580        590        600 
VWKDIYGIDE KKIASMVRED KIDILVELTG HTANNKLGTM ACRPAPVQVT WIGYPNTTGL 

       610        620        630        640        650        660 
PTVDYRITDS LADPPDTKQK QVEELVRLPD CFLCYTPSPE AGPVCPTPAL SNGFVTFGSF 

       670        680        690        700        710        720 
NNLAKITPKV LQVWARILCA VPNSRLVVKC KPFCCDSIRQ RFLTTLEQLG LESKRVDLLP 

       730        740        750        760        770        780 
LILFNHDHMQ AYSLMDISLD TFPYAGTTTT CESLYMGVPC VTMAGSVHAH NVGVSLLTKV 

       790        800        810        820        830        840 
GLGHLVAKNE DEYVQLSVDL ASDVTALSKL RMSLRDLMAG SPVCNGPSFA VGLESAYRNM 

       850        860        870        880        890        900 
WKKYCKGEVP SLRRMEMLQK EVHDDPLISK DLGPSRVSVT GEATPSLKAN GSAPVPSSLP 

       910 
TQSPQLSKRM DSTS 

Q96301 in FASTA format

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