[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, AND ENZYME REGULATION. DOI=10.1101/gad.1255404; PubMed=15574588 [NCBI, ExPASy, EBI, Israel, Japan] Apfeld J., O'Connor G., McDonagh T., DiStefano P.S., Curtis R.; "The AMP-activated protein kinase AAK-2 links energy levels and insulin-like signals to lifespan in C. elegans."; Genes Dev. 18:3004-3009(2004).
[2]	ERRATUM. Apfeld J., O'Connor G., McDonagh T., DiStefano P.S., Curtis R.; Genes Dev. 19:188-188(2005).
[3]	ERRATUM. Apfeld J., O'Connor G., McDonagh T., DiStefano P.S., Curtis R.; Genes Dev. 19:411-411(2005).
[4]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING. STRAIN=Bristol N2; DOI=10.1126/science.282.5396.2012; PubMed=9851916 [NCBI, ExPASy, EBI, Israel, Japan] The C. elegans sequencing consortium; "Genome sequence of the nematode C. elegans: a platform for investigating biology."; Science 282:2012-2018(1998).

Comments

FUNCTION: Acts as a sensor that couples lifespan to information about energy levels and insulin-like signals.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
ENZYME REGULATION: Activated by phosphorylation.
INTERACTION:
Q20632:-; NbExp=1; IntAct=EBI-326514, EBI-326535;
Q86S66:-; NbExp=1; IntAct=EBI-326514, EBI-326549;
Q18885:icd-1; NbExp=1; IntAct=EBI-326514, EBI-326530;
Q10902:nhr-2; NbExp=1; IntAct=EBI-326514, EBI-312197;
Q20455:unc-42; NbExp=1; IntAct=EBI-326514, EBI-326539;

ALTERNATIVE PRODUCTS: 3 named isoforms [FASTA] produced by alternative splicing.

Name	b
Isoform ID	Q95ZQ4-1
Note: No experimental confirmation available.
This is the isoform sequence displayed in this entry.

Name	a
Isoform ID	Q95ZQ4-2
Features which should be applied to build the isoform sequence: VSP_051941.

Name	c
Isoform ID	Q95ZQ4-3
Note: No experimental confirmation available.
Features which should be applied to build the isoform sequence: VSP_051940, VSP_051941.

SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
SIMILARITY: Contains 1 protein kinase domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AY347273; AAR06928.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U58726; AAM69095.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U58726; AAM69096.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U58726; AAP13770.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

T29858; T29858.

NP_510710.2; -.

3D structure databases

HSSP

P49137; 1NXK. [HSSP ENTRY / PDB]

SMR

Q95ZQ4; 81-349.

ModBase

Q95ZQ4.

Protein-protein interaction databases

IntAct

Q95ZQ4; -.

Organism-specific databases

WormBase

WBGene00020142; aak-2.

WormPep

T01C8.1a; CE31222. [WormPep / WorfDB]
T01C8.1b; CE31223. [WormPep / WorfDB]
T01C8.1c; CE07458. [WormPep / WorfDB]

Gene expression databases

ArrayExpress

Q95ZQ4; -.

Ontologies

GO:0005515;	Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0008340;	Biological process: determination of adult life span (inferred from mutant phenotype from WormBase).
QuickGo view.

Family and domain databases

InterPro

IPR015741; AMPK.
IPR000719; Prot_kinase_core.
IPR017441; Protein_kinase_ATP_bd_CS.
IPR017442; Se/Thr_pkinase-rel.
IPR008271; Ser_thr_pkin_AS.
IPR002290; Ser_thr_pkinase.
Graphical view of domain structure.

PANTHER

PTHR22982:SF61; AMPK; 1.

Pfam

PF00069; Pkinase; 1.
Pfam graphical view of domain structure.

ProDom

PD000001; Prot_kinase; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00220; S_TKc; 1.
SMART graphical view of domain structure.

PROSITE

PS00107; PROTEIN_KINASE_ATP; 1.
PS50011; PROTEIN_KINASE_DOM; 1.
PS00108; PROTEIN_KINASE_ST; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q95ZQ4.

Genome annotation databases

Ensembl

T01C8.1; Caenorhabditis elegans. [Contig view]

GeneID

181727; -.

Other

ProtoNet

Q95ZQ4.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Serine/threonine-protein kinase; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 626`	`626`	`5'-AMP-activated protein kinase catalytic subunit alpha-2.`	`PRO_0000085599`
`DOMAIN`	`87 339`	`253`	`Protein kinase.`
`NP_BIND`	`93 101`	`9`	`ATP (By similarity).`
`ACT_SITE`	`210 210`		`Proton acceptor (By similarity).`
`BINDING`	`116 116`		`ATP (By similarity).`
`VAR_SEQ`	`1 62`		`Missing (in isoform c).`	`VSP_051940`
`VAR_SEQ`	`370 371`		`Missing (in isoform a and isoform c).`	`VSP_051941`

Sequence information

Length: 626 AA [This is the length of the unprocessed precursor]

Molecular weight: 70443 Da [This is the MW of the unprocessed precursor]

CRC64: D1401DB1286D8624 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MFSHQDRDRD RKEDGGGDGT EMKSKSRSQP SGLNRVKNLS RKLSAKSRKE RKDRDSTDNS 

        70         80         90        100        110        120 
SKMSSPGGET STKQQQELKA QIKIGHYILK ETLGVGTFGK VKVGIHETTQ YKVAVKILNR 

       130        140        150        160        170        180 
QKIKSLDVVG KIRREIQNLS LFRHPHIIRL YQVISTPSDI FMIMEHVSGG ELFDYIVKHG 

       190        200        210        220        230        240 
RLKTAEARRF FQQIISGVDY CHRHMVVHRD LKPENLLLDE QNNVKIADFG LSNIMTDGDF 

       250        260        270        280        290        300 
LRTSCGSPNY AAPEVISGKL YAGPEVDVWS CGVILYALLC GTLPFDDEHV PSLFRKIKSG 

       310        320        330        340        350        360 
VFPTPDFLER PIVNLLHHML CVDPMKRATI KDVIAHEWFQ KDLPNYLFPP INESEASIVD 

       370        380        390        400        410        420 
IEAVREVTEF QRYHVAEEEV TSALLGDDPH HHLSIAYNLI VDNKRIADET AKLSIEEFYQ 

       430        440        450        460        470        480 
VTPNKGPGPV HRHPERIAAS VSSKITPTLD NTEASGANRN KRAKWHLGIR SQSRPEDIMF 

       490        500        510        520        530        540 
EVFRAMKQLD MEWKVLNPYH VIVRRKPDAP AADPPKMSLQ LYQVDQRSYL LDFKSLADEE 

       550        560        570        580        590        600 
SGSASASSSR HASMSMPQKP AGIRGTRTSS MPQAMSMEAS IEKMEVHDFS DMSCDVTPPP 

       610        620 
SPGGAKLSQT MQFFEICAAL IGTLAR

Q95ZQ4 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools