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UniProtKB/Swiss-Prot entry Q94BT6


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ADO1_ARATH
Primary accession number Q94BT6
Secondary accession number Q9LDF6
Integrated into Swiss-Prot on November 8, 2005
Sequence was last modified on November 8, 2005 (Sequence version 2)
Annotations were last modified on    April 29, 2008 (Entry version 45)
Name and origin of the protein
Protein name Adagio protein 1
Synonyms Protein ZEITLUPE
LOV kelch protein 1
Flavin-binding kelch repeat F-box protein 1-like protein 2
FKF1-like protein 2
F-box only protein 2b
FBX2b
Clock-associated PAS protein ZTL
Gene name
Name: ADO1
Synonyms: FKL2, LKP1, ZTL
OrderedLocusNames: At5g57360
ORFNames: MSF19.2
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, AND MUTAGENESIS OF ASP-320 AND ASP-425.
DOI=10.1016/S0092-8674(00)80841-7; PubMed=10847686 [NCBI, ExPASy, EBI, Israel, Japan]
Somers D.E., Schultz T.F., Milnamow M., Kay S.A.;
"ZEITLUPE encodes a novel clock-associated PAS protein from Arabidopsis.";
Cell 101:319-329(2000).
[2]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
DOI=10.1016/S0092-8674(00)80842-9; PubMed=10847687 [NCBI, ExPASy, EBI, Israel, Japan]
Nelson D.C., Lasswell J.E., Rogg L.E., Cohen M.A., Bartel B.;
"FKF1, a clock-controlled gene that regulates the transition to flowering in Arabidopsis.";
Cell 101:331-340(2000).
[3]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND INDUCTION.
DOI=10.1046/j.1365-313x.2000.00850.x; PubMed=10998191 [NCBI, ExPASy, EBI, Israel, Japan]
Kiyosue T., Wada M.;
"LKP1 (LOV kelch protein 1): a factor involved in the regulation of flowering time in Arabidopsis.";
Plant J. 23:807-815(2000).
[4]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INTERACTION WITH CRY1 AND PHYB.
DOI=10.1038/35068589; PubMed=11260718 [NCBI, ExPASy, EBI, Israel, Japan]
Jarillo J.A., Capel J., Tang R.-H., Yang H.-Q., Alonso J.M., Ecker J.R., Cashmore A.R.;
"An Arabidopsis circadian clock component interacts with both CRY1 and phyB.";
Nature 410:487-490(2001).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1093/dnares/5.6.379; PubMed=10048488 [NCBI, ExPASy, EBI, Israel, Japan]
Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones.";
DNA Res. 5:379-391(1998).
[6]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[7]
GENE FAMILY, AND NOMENCLATURE.
DOI=10.1016/S1360-1385(00)01769-6; PubMed=11077244 [NCBI, ExPASy, EBI, Israel, Japan]
Xiao W., Jang J.-C.;
"F-box proteins in Arabidopsis.";
Trends Plant Sci. 5:454-457(2000).
[8]
INTERACTION WITH SKP1A; SKP1B; SKP1D; SKP1K AND SKP1S.
DOI=10.1046/j.1365-313X.2003.01768.x; PubMed=12795696 [NCBI, ExPASy, EBI, Israel, Japan]
Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L.;
"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis.";
Plant J. 34:753-767(2003).
[9]
INDUCTION.
DOI=10.1073/pnas.0736949100; PubMed=12665620 [NCBI, ExPASy, EBI, Israel, Japan]
Kim W.-Y., Geng R., Somers D.E.;
"Circadian phase-specific degradation of the F-box protein ZTL is mediated by the proteasome.";
Proc. Natl. Acad. Sci. U.S.A. 100:4933-4938(2003).
[10]
INTERACTION WITH SKP1A; SKP1B; SKP1D; SKP1K; SKP1N; ADO2; APRR1 AND APRR5.
DOI=10.1093/jxb/erh226; PubMed=15310821 [NCBI, ExPASy, EBI, Israel, Japan]
Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T.;
"Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis.";
J. Exp. Bot. 55:2015-2027(2004).
[11]
FUNCTION.
DOI=10.1105/tpc.016808; PubMed=14973171 [NCBI, ExPASy, EBI, Israel, Japan]
Somers D.E., Kim W.-Y., Geng R.;
"The F-box protein ZEITLUPE confers dosage-dependent control on the circadian clock, photomorphogenesis, and flowering time.";
Plant Cell 16:769-782(2004).
[12]
FUNCTION, MUTAGENESIS OF LEU-200 AND LEU-213, AND IDENTIFICATION IN A SCF(ADO1) COMPLEX.
DOI=10.1111/j.1365-313X.2004.02207.x; PubMed=15447654 [NCBI, ExPASy, EBI, Israel, Japan]
Han L., Mason M., Risseeuw E.P., Crosby W.L., Somers D.E.;
"Formation of an SCF(ZTL) complex is required for proper regulation of circadian timing.";
Plant J. 40:291-301(2004).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF254413; AAF70288.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF216525; AAF32300.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB038796; BAB18914.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF252294; AAK27433.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB016891; BAB08473.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY039902; AAK64006.1; ALT_FRAME; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT008772; AAP68211.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_568855.1; -.
UniGene At.21022
3D structure databases
HSSP Q8LPE0; 1N9L. [HSSP ENTRY / PDB]
ModBase Q94BT6.
Protein-protein interaction databases
IntAct Q94BT6; -.
Organism-specific databases
GeneFarm 5106; 485.
TAIR At5g57360; -.
Gene expression databases
GermOnline AT5G57360; Arabidopsis thaliana.
Ontologies
GO
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR001810; F-box.
IPR015915; Kelch-typ_b-propeller.
IPR006652; Kelch_1.
IPR011498; Kelch_2.
IPR001610; PAC.
IPR000014; PAS.
IPR000700; PAS-assoc_C.
Graphical view of domain structure.
Gene3D G3DSA:2.120.10.80; Kelch-typ_b-propeller; 1.
Pfam PF00646; F-box; 1.
PF01344; Kelch_1; 1.
PF07646; Kelch_2; 4.
Pfam graphical view of domain structure.
SMART SM00256; FBOX; 1.
SM00086; PAC; 1.
SM00091; PAS; 1.
SMART graphical view of domain structure.
PROSITE PS50181; FBOX; 1.
PS50113; PAC; FALSE_NEG.
PS50112; PAS; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q94BT6.
Genome annotation databases
GeneID 835842; -.
GenomeReviews BA000015_GR; AT5G57360.
KEGG ath:AT5G57360; -.
NMPDR fig|3702.1.peg.27688; -.
Other
ProtoNet Q94BT6.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Biological rhythms; Chromophore; Complete proteome; Cytoplasm; Flavoprotein; FMN; Kelch repeat; Nucleus; Photoreceptor protein; Receptor; Repeat; Sensory transduction; Ubl conjugation; Ubl conjugation pathway.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   609  609     Adagio protein 1. PRO_0000119956
DOMAIN   32   114  83     PAS. 
DOMAIN   118   161  44     PAC. 
DOMAIN   195   241  47     F-box. 
REPEAT   292   342  51     Kelch 1. 
REPEAT   345   392  48     Kelch 2. 
REPEAT   397   445  49     Kelch 3. 
REPEAT   450   501  52     Kelch 4. 
REPEAT   516   564  49     Kelch 5. 
MOD_RES   82    82        S-4a-FMN cysteine (By similarity). 
MUTAGEN   200   200        L->A: No SCF(ADO1) complex formation and reduced cyclic degradation of ADO1; when associated with A-213. 
MUTAGEN   213   213        L->A: No SCF(ADO1) complex formation and reduced cyclic degradation of ADO1; when associated with A-200. 
MUTAGEN   320   320        D->N: In ztl-2; affects circadian clock by lengthening the free-running period of clock-controlled processes. 
MUTAGEN   425   425        D->N: In ztl-1; affects circadian clock by lengthening the free-running period of clock-controlled processes. 
Sequence information
Length: 609 AA [This is the length of the unprocessed precursor] Molecular weight: 65906 Da [This is the MW of the unprocessed precursor] CRC64: B25192FCBE019093 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MEWDSGSDLS ADDASSLADD EEGGLFPGGG PIPYPVGNLL HTAPCGFVVT DAVEPDQPII 

        70         80         90        100        110        120 
YVNTVFEMVT GYRAEEVLGG NCRFLQCRGP FAKRRHPLVD SMVVSEIRKC IDEGIEFQGE 

       130        140        150        160        170        180 
LLNFRKDGSP LMNRLRLTPI YGDDDTITHI IGIQFFIETD IDLGPVLGSS TKEKSIDGIY 

       190        200        210        220        230        240 
SALAAGERNV SRGMCGLFQL SDEVVSMKIL SRLTPRDVAS VSSVCRRLYV LTKNEDLWRR 

       250        260        270        280        290        300 
VCQNAWGSET TRVLETVPGA KRLGWGRLAR ELTTLEAAAW RKLSVGGSVE PSRCNFSACA 

       310        320        330        340        350        360 
VGNRVVLFGG EGVNMQPMND TFVLDLNSDY PEWQHVKVSS PPPGRWGHTL TCVNGSNLVV 

       370        380        390        400        410        420 
FGGCGQQGLL NDVFVLNLDA KPPTWREISG LAPPLPRSWH SSCTLDGTKL IVSGGCADSG 

       430        440        450        460        470        480 
VLLSDTFLLD LSIEKPVWRE IPAAWTPPSR LGHTLSVYGG RKILMFGGLA KSGPLKFRSS 

       490        500        510        520        530        540 
DVFTMDLSEE EPCWRCVTGS GMPGAGNPGG VAPPPRLDHV AVNLPGGRIL IFGGSVAGLH 

       550        560        570        580        590        600 
SASQLYLLDP TEDKPTWRIL NIPGRPPRFA WGHGTCVVGG TRAIVLGGQT GEEWMLSELH 


ELSLASYLT 

Q94BT6 in FASTA format

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