ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry Q93W20


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name NIFU2_ARATH
Primary accession number Q93W20
Secondary accession number Q9LTX6
Integrated into Swiss-Prot on July 25, 2006
Sequence was last modified on December 1, 2001 (Sequence version 1)
Annotations were last modified on    June 16, 2009 (Entry version 41)
Name and origin of the protein
Protein name NifU-like protein 2, chloroplastic [Precursor]
Synonyms AtCNfu2
AtCnfU-V
Gene name
Name: NIFU2
Synonyms: CNFU2, NFU2
OrderedLocusNames: At5g49940
ORFNames: K9P8.16
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND GENE FAMILY.
STRAIN=cv. Columbia;
DOI=10.1042/BJ20021946; PubMed=12553879 [NCBI, ExPASy, EBI, Israel, Japan]
Leon S., Touraine B., Ribot C., Briat J.-F., Lobreaux S.;
"Iron-sulphur cluster assembly in plants: distinct NFU proteins in mitochondria and plastids from Arabidopsis thaliana.";
Biochem. J. 371:823-830(2003).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1093/dnares/7.1.31; PubMed=10718197 [NCBI, ExPASy, EBI, Israel, Japan]
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones.";
DNA Res. 7:31-63(2000).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1071006; PubMed=11910074 [NCBI, ExPASy, EBI, Israel, Japan]
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.;
"Functional annotation of a full-length Arabidopsis cDNA collection.";
Science 296:141-145(2002).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[5]
FUNCTION, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
DOI=10.1105/tpc.020511; PubMed=15031412 [NCBI, ExPASy, EBI, Israel, Japan]
Yabe T., Morimoto K., Kikuchi S., Nishio K., Terashima I., Nakai M.;
"The Arabidopsis chloroplastic NifU-like protein CnfU, which can act as an iron-sulfur cluster scaffold protein, is required for biogenesis of ferredoxin and photosystem I.";
Plant Cell 16:993-1007(2004).
[6]
FUNCTION.
DOI=10.1111/j.1365-313X.2004.02189.x; PubMed=15361144 [NCBI, ExPASy, EBI, Israel, Japan]
Touraine B., Boutin J.-P., Marion-Poll A., Briat J.-F., Peltier G., Lobreaux S.;
"Nfu2: a scaffold protein required for [4Fe-4S] and ferredoxin iron-sulphur cluster assembly in Arabidopsis chloroplasts.";
Plant J. 40:101-111(2004).
Comments
  • FUNCTION: Molecular scaffold for [Fe-S] cluster assembly of chloroplastic iron-sulfur proteins. Required for biogenesis of ferredoxin, a major photosynthetic electron carrier containing [2Fe-2S] cluster. Required for the assembly of photosystem I complex.
  • COFACTOR: Binds 1 [2Fe-2S] cluster per subunit.
  • SUBUNIT: Homodimer.
  • SUBCELLULAR LOCATION: Plastid, chloroplast stroma.
  • ALTERNATIVE PRODUCTS: 1 named isoform [FASTA] produced by alternative splicing. A number of isoforms are produced. According to EST sequences.
    Name1
    Isoform IDQ93W20-1
    This is the isoform sequence displayed in this entry.
  • TISSUE SPECIFICITY: Predominantly expressed in leaves and floral stalks. Ubiquitous (at protein level).
  • DISRUPTION PHENOTYPE: Plants are dwarf with faint pale-green leaves, decreased amount of ferredoxin and impaired photosystem I accumulation.
  • SIMILARITY: Belongs to the nifU family.
  • SEQUENCE CAUTION:
    • Sequence=BAA97015.1; Type=Erroneous gene model prediction; Note=The predicted gene At5g49940 has been split into 2 genes: At5g49940 and At5g49945
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AJ512934; CAD55559.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB024032; BAA97015.1; ALT_SEQ; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF370353; AAK44168.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF428399; AAL16167.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY062984; AAL34158.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK118652; BAC43248.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00517598; -.
RefSeq NP_568715.1; -.
UniGene At.26219
3D structure databases
PDB
2Z51; X-ray; 1.35 A; A=83-235.[ExPASy / RCSB / EBI]
PDBsum 2Z51; -.
ModBase Q93W20.
Organism-specific databases
GeneFarm 3943; 402.
TAIR At5g49940; -.
Gene expression databases
ArrayExpress Q93W20; -.
GermOnline AT5G49940; Arabidopsis thaliana.
Ontologies
GO
GO:0009570; Cellular component: chloroplast stroma (inferred from electronic annotation from UniProtKB-SubCell).
GO:0051537; Molecular function: 2 iron, 2 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0005198; Molecular function: structural molecule activity (traceable author statement from TAIR).
GO:0009658; Biological process: chloroplast organization (inferred from mutant phenotype from TAIR).
GO:0016226; Biological process: iron-sulfur cluster assembly (inferred from mutant phenotype from TAIR).
QuickGo view.
Family and domain databases
InterPro IPR001075; NIF_FeS_clus_asmbl_NifU_C.
Graphical view of domain structure.
Pfam PF01106; NifU; 2.
Pfam graphical view of domain structure.
ProDom PD002830; NifU_C; 2.
[Domain structure / List of seq. sharing at least 1 domain]
Proteomic databases
PRIDE Q93W20; -.
Genome annotation databases
GeneID 835057; -.
GenomeReviews BA000015_GR; AT5G49940.
NMPDR fig|3702.1.peg.26841; -.
Phylogenomic databases
OMA Q93W20; LHEIDGN.
Other
ProtoNet Q93W20.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
2Fe-2S; 3D-structure; Alternative splicing; Chloroplast; Complete proteome; Iron; Iron-sulfur; Metal-binding; Plastid; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom  To Length Description FTId
TRANSIT   1    16  16     Chloroplast (Potential). 
CHAIN   17   235  219     NifU-like protein 2, chloroplastic. PRO_0000247613
Sequence information
Length: 235 AA [This is the length of the unprocessed precursor] Molecular weight: 25620 Da [This is the MW of the unprocessed precursor] CRC64: 6443A3A407D8A3A8 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MQLLTLNPAA ISRTPPQAID PSSSSSLLLP FPQILSSQRA LGLVARPCNP LRRGLSRFLS 

        70         80         90        100        110        120 
SRQLFRRSKV VKAVATPDPI LEVPLTEENV ESVLDEIRPY LMSDGGNVAL HEIDGNIVRV 

       130        140        150        160        170        180 
KLQGACGSCP SSTMTMKMGI ERRLMEKIPE IVAVEALPDE ETGLELNEEN IEKVLEEIRP 

       190        200        210        220        230 
YLIGTADGSL DLVEIEDPIV KIRITGPAAG VMTVRVAVTQ KLREKIPSIA AVQLI 

Q93W20 in FASTA format

View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by ch flag SIB Switzerland Mirror sites: Australia  Brazil  Canada  China  Korea
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!