[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT). DOI=10.1016/0378-1119(95)00153-W; PubMed=7642098 [NCBI, ExPASy, EBI, Israel, Japan] Zhang X., Wright C.V., Hanks S.K.; "Cloning of a Xenopus laevis cDNA encoding focal adhesion kinase (FAK) and expression during early development."; Gene 160:219-222(1995).
[2]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG). TISSUE=Embryo; DOI=10.1006/dbio.1995.1214; PubMed=7649362 [NCBI, ExPASy, EBI, Israel, Japan] Hens M.D., DeSimone D.W.; "Molecular analysis and developmental expression of the focal adhesion kinase pp125FAK in Xenopus laevis."; Dev. Biol. 170:274-288(1995).

Comments

FUNCTION: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy (By similarity).
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBCELLULAR LOCATION: Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Constituent of focal adhesions.

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name	Long
Isoform ID	Q91738-1
This is the isoform sequence displayed in this entry.

Name	Short
Isoform ID	Q91738-2
Features which should be applied to build the isoform sequence: VSP_004978, VSP_004979, VSP_004980.

DEVELOPMENTAL STAGE: Present in the fertilized egg and in cleavage and blastula stage embryos. During gastrulation, expression increases significantly and is detected in mesoderm, marginal zone ectoderm, and cells of the blastocoel roof. Later in development, prominently expressed at intersomitic junctions, in the brain and in several cranial nerves.
PTM: Phosphorylated on tyrosine residues; phosphorylated kinase is first detected during gastrulation, suggesting that tyrosine phosphorylation is developmentally regulated.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
SIMILARITY: Contains 1 FERM domain.
SIMILARITY: Contains 1 protein kinase domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L33920; AAA99456.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U11078; AAA80333.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

I51670; I51670.
JC4200; JC4200.

RefSeq

NP_001084066.1; -.

UniGene

Xl.6819

3D structure databases

HSSP

Q05397; 1K05. [HSSP ENTRY / PDB]

SMR

Q91738; 33-383, 428-699, 924-1065.

ModBase

Q91738.

Organism-specific databases

Xenbase

XB-FEAT-952024; ptk2.

Ontologies

GO:0005856;	Cellular component: cytoskeleton (inferred from electronic annotation from InterPro).
GO:0005925;	Cellular component: focal adhesion (inferred from electronic annotation from InterPro).
GO:0005524;	Molecular function: ATP binding (inferred from electronic annotation from InterPro).
GO:0004715;	Molecular function: non-membrane spanning protein tyrosine kinase activity (inferred from electronic annotation from EC).
GO:0004871;	Molecular function: signal transducer activity (inferred from electronic annotation from InterPro).
GO:0007275;	Biological process: multicellular organismal development (inferred from electronic annotation from UniProtKB-KW).
GO:0006468;	Biological process: protein amino acid phosphorylation (inferred from electronic annotation from InterPro).
GO:0007172;	Biological process: signal complex assembly (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR000299; Band_4.1_N.
IPR005189; Focal_AT.
IPR000719; Prot_kinase_core.
IPR017441; Protein_kinase_ATP_bd_CS.
IPR001245; Tyr_pkinase.
IPR008266; Tyr_pkinase_AS.
Graphical view of domain structure.

Pfam

PF03623; Focal_AT; 1.
PF07714; Pkinase_Tyr; 1.
Pfam graphical view of domain structure.

PRINTS

PR00109; TYRKINASE.

ProDom

PD000001; Prot_kinase; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00295; B41; 1.
SM00219; TyrKc; 1.
SMART graphical view of domain structure.

PROSITE

PS00660; FERM_1; FALSE_NEG.
PS00661; FERM_2; 1.
PS50057; FERM_3; 1.
PS00107; PROTEIN_KINASE_ATP; 1.
PS50011; PROTEIN_KINASE_DOM; 1.
PS00109; PROTEIN_KINASE_TYR; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

Q91738.

Genome annotation databases

GeneID

399286; -.

KEGG

xla:399286; -.

Phylogenomic databases

HOVERGEN

Q91738; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; ATP-binding; Cell junction; Cell membrane; Developmental protein; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Transferase; Tyrosine-protein kinase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1068`	`1068`	`Focal adhesion kinase 1.`	`PRO_0000088080`
`DOMAIN`	`35 355`	`321`	`FERM.`
`DOMAIN`	`435 693`	`259`	`Protein kinase.`
`NP_BIND`	`441 449`	`9`	`ATP (By similarity).`
`COMPBIAS`	`725 746`	`22`	`Pro-rich.`
`COMPBIAS`	`876 929`	`54`	`Pro-rich.`
`ACT_SITE`	`559 559`		`Proton acceptor (By similarity).`
`BINDING`	`467 467`		`ATP (By similarity).`
`MOD_RES`	`403 403`		`Phosphotyrosine (By similarity).`
`MOD_RES`	`413 413`		`Phosphotyrosine (By similarity).`
`MOD_RES`	`589 589`		`Phosphotyrosine; by autocatalysis (By similarity).`
`MOD_RES`	`590 590`		`Phosphotyrosine; by autocatalysis (By similarity).`
`MOD_RES`	`874 874`		`Phosphotyrosine (By similarity).`
`MOD_RES`	`941 941`		`Phosphotyrosine (By similarity).`
`VAR_SEQ`	`393 398`		`Missing (in isoform Short).`	`VSP_004978`
`VAR_SEQ`	`418 425`		`KSYGLDEA -> T (in isoform Short).`	`VSP_004979`
`VAR_SEQ`	`917 919`		`Missing (in isoform Short).`	`VSP_004980`
`CONFLICT`	`439 439`		`R -> P (in Ref. 2; AAA99456).`

Sequence information

Length: 1068 AA [This is the length of the unprocessed precursor]

Molecular weight: 121156 Da [This is the MW of the unprocessed precursor]

CRC64: EF5BEBD60D2C99DA [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAAAYLDPNL NHNPSTNAKS RLSTGMERSP GAIERVLRVF HYFESNNEPA TWSSNIRHGD 

        70         80         90        100        110        120 
ATDVRGIIQK IVDSHKVKNV ASYGLRLSHL HSEEVHWLHP DIGVSHIREK YEQSHPPEEW 

       130        140        150        160        170        180 
KYELRIRYLP KGFVNQFTED KPTLNFFYQQ VKNDYMSEIA DQVDQEIALK LGCLEIRRSY 

       190        200        210        220        230        240 
GEMRGNALEK KSNYEVLEKD VGLKRFFPKS LLDSVKAKTL RKLIQQTFRQ FANLNREESI 

       250        260        270        280        290        300 
LKFFEILSPV YRYDKECFKC ALGSSWIISV ELAIGPEEGI SYLTDKGSNP THLADFTQVQ 

       310        320        330        340        350        360 
TIQYSSSEDK DRKGMLQLKI AGAPEPLTVT APSLTIAENM ADLIDGYCRL VSGASESFII 

       370        380        390        400        410        420 
RPQKEGERAL PSIPKLANNE KHGVRPHAVS VSDEFSGDET DDYAEIIDEE DTYTMPSKSY 

       430        440        450        460        470        480 
GLDEAGDYEI QRDRIELGRC IGEGQFGDVH QGVYMSPENP AMAVAIKTCK NCTSDSVREK 

       490        500        510        520        530        540 
FLQEALTMRQ FDHPHIVKLI GVITENPVWI IMELCTLGEL RSFLQVRKYS LDLASLILYS 

       550        560        570        580        590        600 
YQLSTALAYL ESKRFVHRDI AARNVLVSSS DCVKLGDFGL SRYMEDSTYY KASKGKLPIK 

       610        620        630        640        650        660 
WMAPESINFR RFTSASDVWM FGVCMWEILM YGVKPFQGVK NNDVIGRIEN GERLPMPPNC 

       670        680        690        700        710        720 
PPTLYSLMTK CWAYDPSRRP RFTELKAQLS TILEEEKLQQ EERMRMESRR QVTVSWDSGG 

       730        740        750        760        770        780 
SDEAPPKPSR PGYPSPRSSE GFFPSPQHMM QPNHYQVSGF SVAHGIPSMS GNMYPGQASV 

       790        800        810        820        830        840 
LDHMDSWNHR TPDINMWQPS MEDSGPMDMR SLAQVLPTHL MEERLIRQQQ EMEEDQRWLE 

       850        860        870        880        890        900 
KEERFLKPDV RLSRGSVDHV DGNIQCPAGN QHIYQPVGKP DHVAPPKKPP RPGAPSHLGN 

       910        920        930        940        950        960 
LPAHNSPVDG YNEGVKPWRI QPQEISPPPT ANLDRTNDKV YENVTGLVKA VIEMSSRIQP 

       970        980        990       1000       1010       1020 
APPEEYVPMV KGVGLALRTL LATVDETIPV LPASTHREIE MAQKLLNSDL AELINKMKLA 

      1030       1040       1050       1060 
QQYVMTSLQQ EYKKQMLTAA HALAVDAKNL LDVIDQARLK IISHSRPH

Q91738 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools