NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1073/pnas.201271098; PubMed=11593023 [NCBI, ExPASy, EBI, Israel, Japan]
Adachi N., Ishino T., Ishii Y., Takeda S., Koyama H.;
"DNA ligase IV-deficient cells are more resistant to ionizing radiation in the absence of Ku70: implications for DNA double-strand break repair.";
Proc. Natl. Acad. Sci. U.S.A. 98:12109-12113(2001).

[2]

NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=CB;
TISSUE=Bursa of Fabricius;
DOI=10.1186/gb-2004-6-1-r6; PubMed=15642098 [NCBI, ExPASy, EBI, Israel, Japan]
Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M.;
"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.";
Genome Biol. 6:RESEARCH006.1-RESEARCH006.9(2005).

Comments

FUNCTION: Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination (By similarity).
CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
SUBCELLULAR LOCATION: Nucleus (By similarity).
SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
SIMILARITY: Contains 2 BRCT domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB058600; BAB68506.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ720061; CAG31720.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001025987.1; -.

UniGene

Gga.1352

3D structure databases

SMR

Q90YB1; 659-764.

ModBase

Q90YB1.

Family and domain databases

InterPro

IPR001357; BRCT.
IPR000977; DNA_ligase.
IPR012309; DNA_ligase_A_C.
IPR012310; DNA_ligase_A_M.
IPR012308; DNA_ligase_A_N.
IPR016059; DNA_ligase_CS.
IPR012340; NA-bd_OB-fold.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.50.140; OB_NA_bd_sub; 1.

Pfam

PF00533; BRCT; 2.
PF04679; DNA_ligase_A_C; 1.
PF01068; DNA_ligase_A_M; 1.
PF04675; DNA_ligase_A_N; 1.
Pfam graphical view of domain structure.

SMART

SM00292; BRCT; 2.
SMART graphical view of domain structure.

TIGRFAMs

TIGR00574; dnl1; 1.

PROSITE

PS50172; BRCT; 2.
PS00697; DNA_LIGASE_A1; 1.
PS00333; DNA_LIGASE_A2; 1.
PS50160; DNA_LIGASE_A3; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q90YB1.

Genome annotation databases

Ensembl

ENSGALG00000016854; Gallus gallus. [Contig view]

GeneID

418764; -.

KEGG

gga:418764; -.

Phylogenomic databases

HOVERGEN

Q90YB1; -.

Other

ProtoNet

Q90YB1.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cell cycle; Cell division; DNA damage; DNA recombination; DNA repair; DNA replication; Ligase; Nucleotide-binding; Nucleus; Repeat.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 912`	`912`	`DNA ligase 4.`	`PRO_0000059579`
`DOMAIN`	`659 748`	`90`	`BRCT 1.`
`DOMAIN`	`809 912`	`104`	`BRCT 2.`
`ACT_SITE`	`278 278`		`N6-AMP-lysine intermediate (By similarity).`
`CONFLICT`	`492 492`		`V -> I (in Ref. 1; BAB68506).`

Sequence information

Length: 912 AA [This is the length of the unprocessed precursor]

Molecular weight: 104450 Da [This is the MW of the unprocessed precursor]

CRC64: CBF2899CA76D212D [This is a checksum on the sequence]

        10         20         30         40         50         60 
MASAPVLQPS PKRTVASHVP FADLCSTLER IQTCKSRPEK TKYFKDFLDS WRKFHSALHQ 

        70         80         90        100        110        120 
KEKDVTDSFY PAMRLILPQL ERERMAYGIK ETMLAKLYIE LLNLPKDGKD AVKLLNYRTP 

       130        140        150        160        170        180 
TGSRGDAGDF AMIAYFVLKP RSPKRGRLTV EQVNELLDAI ANNNAAKNKG LVKKSLLQLI 

       190        200        210        220        230        240 
TQSTALEQKW LIRMIIKDLK LGVSQQTIFS IFHPDAAELH NVTTDLEKVC RQLHDPSVSL 

       250        260        270        280        290        300 
SDVSIMLFSA FKPMLAAIAD VQQIEKQMNN QVFYIETKLD GERMQMHKDG DVYKYFSRNG 

       310        320        330        340        350        360 
FDYTQQFGAS PVDGSLTPFI HNVFKSDIQN CILDGEMMAY NPETQTFMQK GNKFDIKRMV 

       370        380        390        400        410        420 
EDSDLQTCFC VFDVLMINDQ KLAHESLSKR YKILSNVFTP LTGRIHVVHK KSARTRKEVI 

       430        440        450        460        470        480 
DALNEAIDNR EEGIMVKDPM STYKPDKRGE GWLKIKPEYV NGLMDELDLL IVGGYWGKGS 

       490        500        510        520        530        540 
RGGMMSHFLC AVAETPAPNE KPTVFHSICR VGSGYTMKEL YDLGLKLAKH WKPYNRKDPP 

       550        560        570        580        590        600 
CNILCGTEKP EMYIEPCNSV IVQIKAAEIV NSDMYKTDCT LRFPRIEKIR EDKEWYECMT 

       610        620        630        640        650        660 
LDMLEHLRSR AEGKLASKHL YIDEYDEPQE KKRRTVPKVK KVIGIAEQFK APDLSNVNKV 

       670        680        690        700        710        720 
SSMFEDVEFC VMTGMGRYSK SELESRIAEC GGSVVQNPGP DTYCVIVGAE NVRVKNIIAS 

       730        740        750        760        770        780 
NKYDVVKAEW LLQCFQSKML VPWQPAFMIH MSPETREHFA REYDCYGDSY TADTDVAQLK 

       790        800        810        820        830        840 
EVFSRVKDNK KMPLDLIAEL EERYSWNSCK LCIFRGNTIY VDYYAIINKP STKIHGTRLS 

       850        860        870        880        890        900 
IRALELRFYG AKVVPLLEEG VSHVVIGEDH SRVKEMKALR RMFGKKFKIV SELWVTESVK 

       910 
EGVPKNETQF LI

Q90YB1 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools