NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827;
DOI=10.1073/pnas.241636498; PubMed=11792869 [NCBI, ExPASy, EBI, Israel, Japan]
Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H.;
"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum.";
Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002).

Comments

FUNCTION: Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups (By similarity).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N²-methylguanine.
SIMILARITY: Belongs to the TRM1 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AE009831; AAL63614.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_559432.1; -.

3D structure databases

ModBase

Q8ZWT5.

Enzyme and pathway databases

BioCyc

PAER178306:PAE1628-MON; -.

Ontologies

GO:0003723;	Molecular function: RNA binding (inferred from electronic annotation from InterPro).
GO:0004809;	Molecular function: tRNA (guanine-N2-)-methyltransferase activity (inferred from electronic annotation from HAMAP).
GO:0006400;	Biological process: tRNA modification (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00290; -; 1.
PBIL [Tree]

InterPro

IPR002905; TRM_MeTrfase.
Graphical view of domain structure.

PANTHER

PTHR10631; TRM_mtfrase; 1.

Pfam

PF02005; TRM; 1.
Pfam graphical view of domain structure.

ProtoNet

Q8ZWT5.

Genome annotation databases

GeneID

1465863; -.

GenomeReviews

AE009441_GR; PAE1628.

KEGG

pai:PAE1628; -.

NMPDR

fig|178306.1.peg.1122; -.

Phylogenomic databases

HOGENOM

Q8ZWT5; -.

Genome annotation databases

CMR

Q8ZWT5; PAE1628.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Methyltransferase; S-adenosyl-L-methionine; Transferase; tRNA processing.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 363`	`363`		`N(2),N(2)-dimethylguanosine tRNA methyltransferase.`	`PRO_0000147688`

Sequence information

Length: 363 AA [This is the length of the unprocessed precursor]

Molecular weight: 40108 Da [This is the MW of the unprocessed precursor]

CRC64: 7754A6FBFB41E9E7 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MRHLVLRREG GVKFYAPDPE KYGGIYSAPV FYNPAMEKNR TLSTLLLKAY GKGGLVVCEP 

        70         80         90        100        110        120 
LSGTGVRGIR YAVESGVVGK LILNDISKEA VELIKKNLEI NGVDAEVYNE DANVLLHKLK 

       130        140        150        160        170        180 
DSCDVVDIDP FGSPAPFIHG AFRALKEEGL ICATATDTAV LVGRYPRKCL RRYGSVIVKT 

       190        200        210        220        230        240 
PFYIEVGLRN LLGYIARVAA AEDYKITPLM SYWEGHYFRV CAYAARGARD ADDNFHHIAY 

       250        260        270        280        290        300 
IKYERGVRKI LHAQSEGSSG PLWVGPLGDP LIINKMSEIG PYQDFLKILA EEYSISAPWF 

       310        320        330        340        350        360 
YRLPEFAAGG KSPTLKEAVG VLRAAGIYAV RTHMAPDGFK ADGEYGEVLM AFKRYISSAH 


SLQ

Q8ZWT5 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools