ID   CTNB_DICDI              Reviewed;         254 AA.
AC   Q8WSR7; Q54W80;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   23-SEP-2008, entry version 27.
DE   RecName: Full=Countin-2;
DE   Flags: Precursor;
GN   Name=ctnB; ORFNames=DDB_0214993;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR
RP   LOCATION, AND KNOCKOUT.
RX   MEDLINE=21600743; PubMed=11737154;
RX   DOI=10.1046/j.1440-169X.2001.00615.x;
RA   Okuwa T., Katayama T., Takano A., Kodaira K., Yasukawa H.;
RT   "Two cell-counting factors regulate the aggregate size of the cellular
RT   slime mold Dictyostelium discoideum.";
RL   Dev. Growth Differ. 43:735-744(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA   Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA   Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA   Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA   Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA   Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA   Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA   Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA   Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA   Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA   Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA   Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA   Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA   Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA   Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA   Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA   Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Cell-counting factor that limits the minimum size of the
CC       multicellular structure. May up-regulate the expression of both
CC       gp24 and gp80, which mediate cell adhesion.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- DEVELOPMENTAL STAGE: Expressed in the vegetative cells. Increases
CC       during aggregation to mound stages (6-12 hours) and decreases at
CC       late developmental stages (18-24 hours).
CC   -!- MISCELLANEOUS: Cells lacking cntB form a higher number of smaller
CC       aggregates.
CC   -!- SIMILARITY: Belongs to the countin family.
CC   -!- SIMILARITY: Contains 1 saposin B-type domain.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AB067573; BAB84187.1; -; mRNA.
DR   EMBL; AAFI02000033; EAL67512.1; -; Genomic_DNA.
DR   RefSeq; XP_641512.1; -.
DR   GeneID; 3392606; -.
DR   KEGG; ddi:DDB_0214993; -.
DR   dictyBase; DDB0214993; ctnB.
DR   InterPro; IPR008139; SaposinB.
DR   SMART; SM00741; SapB; 1.
DR   PROSITE; PS50015; SAP_B; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Glycoprotein; Secreted; Signal.
FT   SIGNAL        1     19       Potential.
FT   CHAIN        20    254       Countin-2.
FT                                /FTId=PRO_0000327909.
FT   DOMAIN       22    107       Saposin B-type.
FT   CARBOHYD    110    110       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    219    219       N-linked (GlcNAc...) (Potential).
FT   DISULFID     26    103       By similarity.
FT   DISULFID     29     97       By similarity.
FT   DISULFID     56     68       By similarity.
SQ   SEQUENCE   254 AA;  26347 MW;  359482BE25C6D3AF CRC64;
     MMIKYITIAI LFIASLVKAD LQFSLCPTCV DFINNDMGDL EKIISGGIAT SCGAVCSLLP
     NNIEQGACNL LCDIVGIDEF LKVFNNIGED ADPVWICEEL TVCPKNQNSN ATVLTSDVSP
     ASGPHGTTFT IGVAYKVEST LGTGEVAVMV TDPTGSNGFG DAQLIVNTQP GQYSTSFSFA
     ATPSEDEQFP AGVYQVQLMI CEGSCGAKHS VTFNSVYANF TVTSGPSVTG QMTGTGSGSG
     SGSGSSSGAA YLRY
//