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UniProtKB/Swiss-Prot entry Q8U8Z6


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name HUTI_AGRT5
Primary accession number Q8U8Z6
Secondary accession numbers None
Integrated into Swiss-Prot on August 13, 2002
Sequence was last modified on June 1, 2002 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 48)
Name and origin of the protein
Protein name Imidazolonepropionase
Synonyms EC 3.5.2.7
Imidazolone-5-propionate hydrolase
Gene name
Name: hutI
OrderedLocusNames: Atu3934
ORFNames: AGR_L_1824
From
Agrobacterium tumefaciens (strain C58 / ATCC 33970) [TaxID: 176299] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1126/science.1066804; PubMed=11743193 [NCBI, ExPASy, EBI, Israel, Japan]
Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W.;
"The genome of the natural genetic engineer Agrobacterium tumefaciens C58.";
Science 294:2317-2323(2001).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1126/science.1066803; PubMed=11743194 [NCBI, ExPASy, EBI, Israel, Japan]
Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58.";
Science 294:2323-2328(2001).
[3]
X-RAY CRYSTALLOGRAPHY (1.87 ANGSTROMS) IN COMPLEX WITH PRODUCT AND IRON IONS, AND SUBUNIT.
DOI=10.1002/prot.21559; PubMed=17640072 [NCBI, ExPASy, EBI, Israel, Japan]
Tyagi R., Kumaran D., Burley S.K., Swaminathan S.;
"X-ray structure of imidazolonepropionase from Agrobacterium tumefaciens at 1.87 A resolution.";
Proteins 69:652-658(2007).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE007870; AAK89486.2; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR AH3040; AH3040.
D98245; D98245.
RefSeq NP_356701.2; -.
3D structure databases
PDB
2GOK; X-ray; 1.87 A; A/B=2-419.[ExPASy / RCSB / EBI]
2PUZ; X-ray; 1.83 A; A/B=1-419.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 2GOK; -.
2PUZ; -.
ModBase Q8U8Z6.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0050480; Molecular function: imidazolonepropionase activity (inferred from electronic annotation from HAMAP).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0019556; Biological process: histidine catabolic process to glutamate and formamide (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
HAMAP MF_00372; -; 1.
PBIL [Tree]
InterPro IPR006680; Amidohydro_1.
IPR005920; HutI.
Graphical view of domain structure.
Pfam PF01979; Amidohydro_1; 1.
Pfam graphical view of domain structure.
ProDom PD001248; Amidohydro_like; 1.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR01224; hutI; 1.
ProtoNet Q8U8Z6.
Genome annotation databases
GeneID 1135808; -.
GenomeReviews AE007870_GR; Atu3934.
KEGG atc:AGR_L_1824; -.
atu:Atu3934; -.
Phylogenomic databases
HOGENOM Q8U8Z6; -.
Genome annotation databases
CMR Q8U8Z6; Atu3934.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Complete proteome; Cytoplasm; Histidine metabolism; Hydrolase; Iron; Metal-binding; Zinc.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   419  419     Imidazolonepropionase. PRO_0000160942
REGION   331   333  3     Substrate. 
METAL   84    84        Zinc or iron. 
METAL   86    86        Zinc or iron. 
METAL   254   254        Zinc or iron. 
METAL   329   329        Zinc or iron. 
BINDING   93    93        Substrate (By similarity). 
BINDING   106   106        Substrate (By similarity). 
BINDING   156   156        Substrate. 
BINDING   189   189        Substrate (By similarity). 
BINDING   257   257        Substrate (By similarity). 
STRAND   16    25  10      
STRAND   36    45  10      
STRAND   48    54  7      
HELIX   60    62  3      
STRAND   66    70  5      
STRAND   75    78  4      
STRAND   80    85  6      
HELIX   94   101  8      
HELIX   106   111  6      
HELIX   116   125  10      
HELIX   128   143  16      
TURN   144   146  3      
STRAND   147   153  7      
HELIX   160   173  14      
TURN   174   176  3      
STRAND   180   185  6      
HELIX   193   195  3      
HELIX   199   205  7      
HELIX   207   216  10      
STRAND   221   223  3      
STRAND   226   228  3      
HELIX   234   246  13      
STRAND   251   259  9      
HELIX   263   269  7      
STRAND   273   277  5      
HELIX   283   292  10      
STRAND   295   298  4      
HELIX   300   305  6      
HELIX   314   320  7      
STRAND   324   326  3      
TURN   332   334  3      
HELIX   340   351  12      
HELIX   355   361  7      
HELIX   364   369  6      
TURN   373   375  3      
STRAND   376   378  3      
STRAND   387   390  4      
HELIX   397   400  4      
STRAND   407   412  6      
Sequence information
Length: 419 AA [This is the length of the unprocessed precursor] Molecular weight: 44400 Da [This is the MW of the unprocessed precursor] CRC64: 55D09461815693CF [This is a checksum on the sequence]
        10         20         30         40         50         60 
MPGNNSAKGT ATGNATALWR NAQLATLNPA MDGIGAVENA VIAVRNGRIA FAGPESDLPD 

        70         80         90        100        110        120 
DLSTADETTD CGGRWITPAL IDCHTHLVFG GNRAMEFEMR LNGATYEEIA KAGGGIVSSV 

       130        140        150        160        170        180 
RDTRALSDEV LVAQALPRLD TLLSEGVSTI EIKSGYGLDI ETELKMLRVA RRLETLRPVR 

       190        200        210        220        230        240 
IVTSYLAAHA TPADYKGRNA DYITDVVLPG LEKAHAEGLA DAVDGFCEGI AFSVKEIDRV 

       250        260        270        280        290        300 
FAAAQQRGLP VKLHAEQLSN LGGAELAASY NALSADHLEY LDETGAKALA KAGTVAVLLP 

       310        320        330        340        350        360 
GAFYALREKQ LPPVQALRDA GAEIALATDC NPGTSPLTSL LLTMNMGATL FRMTVEECLT 

       370        380        390        400        410 
ATTRNAAKAL GLLAETGTLE AGKSADFAIW DIERPAELVY RIGFNPLHAR IFKGQKVSP 

Q8U8Z6 in FASTA format

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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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