ID   DNE23_AGRT5             Reviewed;        1087 AA.
AC   Q8U642; Q7D2J1;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-NOV-2008, entry version 37.
DE   RecName: Full=Error-prone DNA polymerase 3;
DE            EC=2.7.7.7;
GN   Name=dnaE2-3; OrderedLocusNames=Atu6093; ORFNames=AGR_pTi_175;
OS   Agrobacterium tumefaciens (strain C58 / ATCC 33970).
OG   Plasmid pTiC58.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=21608550; PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P.,
RA   Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L.,
RA   Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr.,
RA   Chapman P., Clendenning J., Deatherage G., Gillet W., Grant C.,
RA   Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A.,
RA   Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D.,
RA   Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M.,
RA   Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M.,
RA   Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V.,
RA   Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=21608551; PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M.,
RA   Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L.,
RA   Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F.,
RA   Wollam C., Allinger M., Doughty D., Scott C., Lappas C., Markelz B.,
RA   Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G.,
RA   Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis
CC       and translesion synthesis (TLS). It is not the major replicative
CC       DNA polymerase (By similarity).
CC   -!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) =
CC       diphosphate + DNA(n+1).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily.
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DR   EMBL; AE007871; AAK91053.1; -; Genomic_DNA.
DR   PIR; AC3239; AC3239.
DR   RefSeq; NP_396612.1; -.
DR   GeneID; 1137416; -.
DR   GenomeReviews; AE007871_GR; Atu6093.
DR   KEGG; atc:AGR_pTi_175; -.
DR   KEGG; atu:Atu6093; -.
DR   HOGENOM; Q8U642; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:HAMAP.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:HAMAP.
DR   GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR   GO; GO:0045020; P:error-prone DNA repair; IEA:HAMAP.
DR   HAMAP; MF_01902; -; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha.
DR   InterPro; IPR004365; NA_bd_OB_tRNA-helicase.
DR   InterPro; IPR004013; PHP_C.
DR   InterPro; IPR003141; PHP_N.
DR   InterPro; IPR004805; PolC_alpha.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Plasmid;
KW   Transferase.
FT   CHAIN         1   1087       Error-prone DNA polymerase 3.
FT                                /FTId=PRO_0000103364.
SQ   SEQUENCE   1087 AA;  122156 MW;  66C81289A3841EC6 CRC64;
     MSYAELQVTT HFSFLRGASS AQELFETAKA LGIEALGVVD RNSLAGIVRA LEASRATGLR
     LVIGCRLDLA DGMSVLVYPT DRAAYSRLTR LITLGKSRGG KNNCLLHWDD VVAYTDGMIG
     ILVPDLPDDL CGIQLRKMAE LFGDRAYVSL CLRRRQNDQL RLHEISNLAT RFKVRTVVTN
     DVLFHEPGRR QLQDIVTCIR TRTTIDEVGF ERERHADRYL KPPEEMERLF PRYRQALART
     MEIVRRCTFS LEELTYQYPE EAIVPGKDAQ ASLEHYVWQC VPDRYPEGLP PDVLKVVRHE
     LDLIRTMKYA PYFLTVFSIV RYARSQGILC QGRGSAANSA VCYILGITSI DPSTNDLLFE
     RFVSQERDEP PDIDVDFEHE RREEVIQWIY RTYTREKAAL CATVTRYRAR GAIRDVGKAL
     GLPEDVIKAL SSGMWSWSEE VCDRNVRELN LNPDDRRLVL TLKLAQQLMG APRHLGQHPG
     GFVLTHDRLD DLVPIEPATM KDRQIIEWDK DDVEALKFMK VDILALGMLT CMAKAFDLIR
     EHKDRDLDLS KIEQEDSVTY AMIRKADTLG TFQIESRAQM AMLPRLKPRT FYDLVVQVAI
     VRPGPIQGDM VHPYLRRREG KEAVEYPTPE LEAVLGKTLG VPLFQESAMR VAMVCAGFTG
     GEADQLRKSM ATFKFTGGVS QFKDKLVSGM VRNGYAPEFA EKTFSQLEGF GSYGFPESHA
     ASFALIAYAS SYIKCHYPEA FCAALINSQP MGFYAPAQIV GDARAHGVEV RPVCINRSRW
     DCTLERIGNS DRHAVRLGFR QVKGLAVADA ARVVAARMNN AFVSVDDMWR RSGVPSEALV
     QLAKADAFLP SLKLERRDAL WAIKALRDEP LPLFAAAAER EMAAIAEQQE PGVALRQMTD
     GHNVIEDYSH TGLTLRQHPI AFLRKDLSVR NIITCAEAMN SRDGRWVYTA GLVLVRQKPG
     SAKGVMFITI EDETGPANLV VWPTLFEKRR RAVLGSSMMA INGRIQREGE VVHLVAQQLF
     DLSGDLTGLA DRDEEFKLPA GRGDEFAHGS PGSSDTRDKS KPVVAPRDIF TPDLHIDTLK
     IKSRNFH
//