[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1; Weiss R.B., Dunn D.M., Robb F.T., Brown J.R.; "The complete sequence of the Pyrococcus furiosus genome."; Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.

Comments

CATALYTIC ACTIVITY: Glycolate + NAD⁺ = glyoxylate + NADH.
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AE010156; AAL80443.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_578048.1; -.

3D structure databases

HSSP

P36234; 1GDH. [HSSP ENTRY / PDB]

SMR

Q8U3Y2; 1-333.

ModBase

Q8U3Y2.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0047964;	Molecular function: glyoxylate reductase activity (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00776; -; 1.
PBIL [Tree]

InterPro

IPR006139; D-isomer_2_OHA_DHase.
IPR006140; D-isomer_2_OHA_DHase_NAD-bd.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

Pfam

PF00389; 2-Hacid_dh; 1.
PF02826; 2-Hacid_dh_C; 1.
Pfam graphical view of domain structure.

PROSITE

PS00065; D_2_HYDROXYACID_DH_1; 1.
PS00670; D_2_HYDROXYACID_DH_2; FALSE_NEG.
PS00671; D_2_HYDROXYACID_DH_3; 1.

BLOCKS

Q8U3Y2.

Genome annotation databases

1468154; -.

AE009950_GR; PF0319.

pfu:PF0319; -.

fig|186497.1.peg.325; -.

Phylogenomic databases

HOGENOM

Q8U3Y2; -.

Genome annotation databases

CMR

Q8U3Y2; PF0319.

Other

ProtoNet

Q8U3Y2.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; NAD; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 336`	`336`	`Glyoxylate reductase.`	`PRO_0000075948`
`NP_BIND`	`158 161`	`4`	`NADP (By similarity).`
`NP_BIND`	`180 182`	`3`	`NADP (By similarity).`
`NP_BIND`	`239 241`	`3`	`NADP (By similarity).`
`NP_BIND`	`288 290`	`3`	`NADP (By similarity).`
`ACT_SITE`	`241 241`		`By similarity.`
`ACT_SITE`	`270 270`		`By similarity.`
`ACT_SITE`	`288 288`		`Proton donor (By similarity).`

Sequence information

Length: 336 AA [This is the length of the unprocessed precursor]

Molecular weight: 38342 Da [This is the MW of the unprocessed precursor]

CRC64: CFC48402FD2073B5 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKPKVFITRA IPENGINMLE EEFEVEVWEE EREIPREKLL EKVKDVDALV TMLSERIDQE 

        70         80         90        100        110        120 
VFENAPRLRI VANYAVGYDN IDVEEATRRG IYVTNTPDVL TNATADHAFA LLLATARHVV 

       130        140        150        160        170        180 
KGDKFVRSGE WKRKGIAWHP KWFLGYELYG KTIGIVGFGR IGQAIARRAK GFNMRILYYS 

       190        200        210        220        230        240 
RTRKSQAEKE LGAEYRPLEE VLKESDFVIL AVPLTKETMY MINEERLKLM KPTAILVNIA 

       250        260        270        280        290        300 
RGKVVDTKAL IKALKEGWIA GAGLDVFEEE PYYNEELFSL DNVVLTPHIG SATFEAREAM 

       310        320        330 
AELVARNLIA FKRGEIPPTL VNKEVIKIRK PGFNEQ

Q8U3Y2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools