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UniProtKB/Swiss-Prot entry Q8TUV3

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DP2L_METKA
Primary accession number Q8TUV3
Secondary accession numbers None
Integrated into Swiss-Prot on October 19, 2002
Sequence was last modified on June 1, 2002 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 27)
Name and origin of the protein
Protein name DNA polymerase II large subunit
Synonyms Pol II
EC 2.7.7.7
Gene name
Name: polC
OrderedLocusNames: MK1650
From
Methanopyrus kandleri [TaxID: 2320] [HAMAP proteome]
Taxonomy Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae; Methanopyrus.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
DOI=10.1073/pnas.032671499; PubMed=11930014 [NCBI, ExPASy, EBI, Israel, Japan]
Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A.;
"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.";
Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002).
Comments
  • FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase (By similarity).
  • CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
  • CATALYTIC ACTIVITY: Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.
  • SUBUNIT: Heterodimer of a large subunit and a small subunit (By similarity).
  • SIMILARITY: Belongs to the archaeal DNA polymerase II family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE010454; AAM02863.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_614933.1; -.
3D structure databases
ModBase Q8TUV3.
Enzyme and pathway databases
BioCyc MKAN190192:MK1650-MON; -.
Ontologies
GO
GO:0008408; Molecular function: 3'-5' exonuclease activity (inferred from electronic annotation from HAMAP).
GO:0003677; Molecular function: DNA binding (inferred from electronic annotation from HAMAP).
GO:0003887; Molecular function: DNA-directed DNA polymerase activity (inferred from electronic annotation from HAMAP).
GO:0000738; Biological process: DNA catabolic process, exonucleolytic (inferred from electronic annotation from HAMAP).
GO:0006261; Biological process: DNA-dependent DNA replication (inferred from electronic annotation from HAMAP).
QuickGo view.
Family and domain databases
HAMAP MF_00324; -; 1.
PBIL [Tree]
InterPro IPR004475; PolC_DP2.
IPR016033; PolC_DP2_N.
Graphical view of domain structure.
Pfam PF03833; PolC_DP2; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF016275; PolC_DP2; 1.
TIGRFAMs TIGR00354; polC; 1.
ProtoNet Q8TUV3.
Genome annotation databases
GeneID 1478245; -.
GenomeReviews AE009439_GR; MK1650.
KEGG mka:MK1650; -.
NMPDR fig|190192.1.peg.1646; -.
Phylogenomic databases
HOGENOM Q8TUV3; -.
Genome annotation databases
CMR Q8TUV3; MK1650.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom To Length Description FTId
CHAIN   1   1240  1240     DNA polymerase II large subunit. PRO_0000152576
Sequence information
Length: 1240 AA [This is the length of the unprocessed precursor] Molecular weight: 140145 Da [This is the MW of the unprocessed precursor] CRC64: 4BEEE6117BCDD9AD [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MNRAREELDR YRETLEEVSS RFVDVATKAR QRREDPKPEP EVMLATSIGE RVEGLLQVEN 

        70         80         90        100        110        120 
VADRLEELEE ELGDREEATF RIVEEVIKGE LKVKGDLPLH KRIDYAVRIG LAVLTEAVVS 

       130        140        150        160        170        180 
APLEGIAAVE IRERGTGHRV VDESEPPHEE PKLVCTECGK EVDPENCYLA VKYAGPIRAA 

       190        200        210        220        230        240 
GGTAAALSAL LADYARQVAG LPRFNPDDFD HDLVGRYVEE VVTYLDKVGS FQYNPSEEEV 

       250        260        270        280        290        300 
ELVAKNIPIE IDGEPTEEVE VQGHRDIPHL PNQLRGGALL VICEGICQKA PKLIKRVEKY 

       310        320        330        340        350        360 
GIDGWEFLEK LVNKGSDDEE EGEEEKTKIK PNDKYMGELV AGRPLLSHPS AKGGFRLRYG 

       370        380        390        400        410        420 
RARNTGFAAV GVHPSLMYVT KGFIVIGTQL KVERPGKAAC VLPVTEIEPP VVKLRDGSVV 

       430        440        450        460        470        480 
RLDDPREAKE LVEKDEIEEI LDLGEMLVAV GEFIENNHPL VPPAYCPEWW VKEVPDVVKV 

       490        500        510        520        530        540 
IGLRKNLPND VFEKLKDVPL KRLVKEASRL SGNGNNLDGF LNGPVKVARS IVELVRKEVI 

       550        560        570        580        590        600 
PRLSSPERMS VEEAIELSRE YGVPFHPKYT FLWHDVKPKD VDELREALEV AGSEWGNLRV 

       610        620        630        640        650        660 
EFENDGEIKR ILEDLLVPHR LEDDTIVVEE PWASALLAQL GYDPESGEFR EQDEDLDYLL 

       670        680        690        700        710        720 
DYLVIRDETC RYVSKLAGFP IREKAPTRIG ARMGRPEKAR ERKMSPPPHV LFPIGIAGGN 

       730        740        750        760        770        780 
QRDIMKFHRG ESEDTDRVEV CYRICPECDR LVPYRVCPFC GTETVQYCNR CDEPADECDC 

       790        800        810        820        830        840 
EEPDPVVRAD IERNDDPYSS LPVRELVRRA EEEVGTTDTL KGVKGMTSRL KMPEPLQKGI 

       850        860        870        880        890        900 
LRAKRDLFVF KDGTLRFDCN DCPLTHVRLK EVGLTPFKAR LLGFERDING DPVVSEDQVV 

       910        920        930        940        950        960 
ELYPQDVVLP RKAAEWAVRV CQYLDDLLRK YYGLEPVYGV EKPEDLIGHL IVTLAPHTSC 

       970        980        990       1000       1010       1020 
GVVGRVVGIA DINCWYNHPI INAARRRNCD GDEDAFMLLL DVLLNFSRLY LPDKRGGLMD 

      1030       1040       1050       1060       1070       1080 
APLVLTAVVD PYEIDDEVWN MDVCGDYPLE LYRKALEYAD AGEAEELIER LEDRLDLPRG 

      1090       1100       1110       1120       1130       1140 
LQFTHDTEAI DLGPTVTRYS RLEKMEEKLE EQLDLARRIR AVDESDVAKI VLDSHFLPDI 

      1150       1160       1170       1180       1190       1200 
KGNLRKFGRQ KFRCSRCNAK FNVPPLSGKC PRCGGDVLLT IYPATATKYL EPAKRLVEEF 

      1210       1220       1230       1240 
GTHDEEWRTI ASEVELLEEE AKTLFGSDHS VSLKKFFGET
Q8TUV3 in FASTA format

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