NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-32, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, AND SUBCELLULAR LOCATION.
STRAIN=Z / UTEX 753;
DOI=10.1038/4151047a; PubMed=11875575 [NCBI, ExPASy, EBI, Israel, Japan]
Iseki M., Matsunaga S., Murakami A., Ohno K., Shiga K., Yoshida K., Sugai M., Takahashi T., Hori T., Watanabe M.;
"A blue-light-activated adenylyl cyclase mediates photoavoidance in Euglena gracilis.";
Nature 415:1047-1051(2002).

[2]

SUBCELLULAR LOCATION.
DOI=10.1039/b502002f; PubMed=16121285 [NCBI, ExPASy, EBI, Israel, Japan]
Ntefidou M., Haeder D.-P.;
"Photoactivated adenylyl cyclase (PAC) genes in the flagellate Euglena gracilis mutant strains.";
Photochem. Photobiol. Sci. 4:732-739(2005).

Comments

FUNCTION: Acts as a blue-light photoreceptor for the step-up photophobic response. Mediates photoavoidance.
CATALYTIC ACTIVITY: ATP = 3',5'-cyclic AMP + diphosphate.
COFACTOR: FAD.
ENZYME REGULATION: Activity increased by up to 80-fold under blue-light.
BIOPHYSICOCHEMICAL PROPERTIES:

Kinetic parameters: K_M=0.5 µM for ATP;
V_max=3.5 nmol/min/mg enzyme;
SUBUNIT: Heterotetramer of two alpha and two beta subunits.
SUBCELLULAR LOCATION: Cell projection, cilium, flagellum. Note=Paraflagellar body, flagellum and paraxonemal bodies (PABs).
SIMILARITY: Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
SIMILARITY: Contains 2 BLUF domains.
SIMILARITY: Contains 2 guanylate cyclase domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB031226; BAB85620.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q8S9F1.

Ontologies

GO:0005929;	Cellular component: cilium (inferred from electronic annotation from UniProtKB-KW).
GO:0019861;	Cellular component: flagellum (inferred from direct assay from UniProtKB).
GO:0004016;	Molecular function: adenylate cyclase activity (inferred from direct assay from UniProtKB).
GO:0009881;	Molecular function: photoreceptor activity (inferred from direct assay from UniProtKB).
GO:0006171;	Biological process: cAMP biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
GO:0007242;	Biological process: intracellular signaling cascade (inferred from electronic annotation from InterPro).
GO:0018298;	Biological process: protein-chromophore linkage (inferred from electronic annotation from UniProtKB-KW).
GO:0050896;	Biological process: response to stimulus (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001054; A/G_cyclase.
IPR007024; BLUF.
Graphical view of domain structure.

Gene3D

G3DSA:3.30.70.1230; A/G_cyclase; 2.

Pfam

PF04940; BLUF; 2.
Pfam graphical view of domain structure.

SMART

SM00044; CYCc; 1.
SMART graphical view of domain structure.

PROSITE

PS50925; BLUF; 2.
PS00452; GUANYLATE_CYCLASE_1; FALSE_NEG.
PS50125; GUANYLATE_CYCLASE_2; 2.
PROSITE graphical view of domain structure (profiles).

ProtoNet

Q8S9F1.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

cAMP biosynthesis; Cell projection; Chromophore; Cilium; Direct protein sequencing; FAD; Flagellum; Flavoprotein; Lyase; Photoreceptor protein; Receptor; Repeat; Sensory transduction.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 859`	`859`	`Photoactivated adenylate cyclase subunit beta.`	`PRO_0000195718`
`DOMAIN`	`56 149`	`94`	`BLUF 1.`
`DOMAIN`	`205 333`	`129`	`Guanylate cyclase 1.`
`DOMAIN`	`471 563`	`93`	`BLUF 2.`
`DOMAIN`	`619 748`	`130`	`Guanylate cyclase 2.`

Sequence information

Length: 859 AA [This is the length of the unprocessed precursor]

Molecular weight: 94221 Da [This is the MW of the unprocessed precursor]

CRC64: C0EF4406D4A8F61E [This is a checksum on the sequence]

        10         20         30         40         50         60 
MYILVWKKGQ QIKTFHTLDE AAQFKAASNI DEAQMFSVTV APAISASGGS NEATNLRRLM 

        70         80         90        100        110        120 
YLSKSTNPEE CNPQFLAEMA RVATIRNREI GVSGFLMYSS PFFFQVIEGT DEDLDFLFAK 

       130        140        150        160        170        180 
ISADPRHERC IVLANGPCTG RMYGDWHMKD SHMDSITTHP AMKTILYQIA RSFSSMWSYL 

       190        200        210        220        230        240 
PKSAGNMLLL GKDPAAQPPE PMSVVVTFIY LVEFGSILSN PNLTEQAAEV LSTFVDVCVK 

       250        260        270        280        290        300 
NVEGSGGNIA KFITGICMAY WPINRTEEAL TAIQQISEDL AQLRSQQAPG SAVSLMYSQA 

       310        320        330        340        350        360 
GVHYGRAMLC NAGSRKSDFT LLGDCINTTS RIATLAKKLK TPLLFSFEVR CLLGDEMREE 

       370        380        390        400        410        420 
IEGAGMHQVK GRDKPVVVYQ FPGPELDVEM VRQKIEQFTP GRFRCQMPVV EYEALPISQR 

       430        440        450        460        470        480 
PPIFDDTPKG NPRPRTPGYG GRQRSDSLVD RLIMIAKLAG PSVSATGDTT LTTLTYISQA 

       490        500        510        520        530        540 
TRPMSRLDLS AIMRTATRRN AQQSITGTLL HVNGLFVQTL EGPKDAVVNL YLRIRQDPRH 

       550        560        570        580        590        600 
TDVTTVHMAP LQERVYPSEW TLTSATEEML ATFPPLQDVL SQLAKSFTSL ETYVPSTVVR 

       610        620        630        640        650        660 
YLTAGNNPRN LMPVSCGVVM LATDICSFTS LTEKSSLTEV WMICNTFIDA CTSAICQEGG 

       670        680        690        700        710        720 
EVIKLIGDCV TAYFPGNNAD SAVAAAQELF TFCRQLREAF VDVLDVRGCV SCGVGLDYGQ 

       730        740        750        760        770        780 
VVMAQCGSLG LTEYVVAGAV SARVMEVEAI TREVGYAIVV TEPVADRLSP QLRDHGIVPT 

       790        800        810        820        830        840 
PQAIEGLPCY GIAGEEFELD VDSIKRGIKA LHAARSGEKP LTEPEEAKPD FRVSPGRVRH 

       850 
GDSGRRSNSA QGKRSIQVR

Q8S9F1 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools