NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395;
Itaya H., Kimura E., Kawahara Y., Sugimoto S.;
"lysC of Corynebacterium efficiens.";
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.

[2]

NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395;
DOI=10.1101/gr.1285603; PubMed=12840036 [NCBI, ExPASy, EBI, Israel, Japan]
Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.;
"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens.";
Genome Res. 13:1572-1579(2003).

Comments

CATALYTIC ACTIVITY: ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
ENZYME REGULATION: Feedback inhibition by lysine and threonine.
PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 1/4 .
PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3 .
PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5 .
SUBUNIT: Tetramer consisting of 2 isoforms Alpha (catalytic) and 2 isoforms Beta (function not known).

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative initiation.

Name	Alpha
Synonyms	Aspartokinase subunit alpha
Isoform ID	Q8RQN1-1
This is the isoform sequence displayed in this entry.

Name	Beta
Synonyms	Aspartokinase subunit beta
Isoform ID	Q8RQN1-2
Features which should be applied to build the isoform sequence: VSP_018656.

SIMILARITY: Belongs to the aspartokinase family.
SIMILARITY: Contains 2 ACT domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB083129; BAB88820.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BA000035; BAC17030.1; ALT_INIT; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_736830.1; -.

3D structure databases

SMR

Q8RQN1; 253-416.

ModBase

Q8RQN1.

Enzyme and pathway databases

BioCyc

CEFF196164:CE0220-MON; -.

Family and domain databases

InterPro

IPR002912; ACT_bd.
IPR001048; Asp/Glu/Uridylate_kinase.
IPR005260; Asp_kin_monofn.
IPR001341; Asp_kin_reg.
IPR001057; Glu_5kinase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.1160.10; Aa_kinase; 1.

Pfam

PF00696; AA_kinase; 1.
PF01842; ACT; 2.
Pfam graphical view of domain structure.

PRINTS

PR00474; GLU5KINASE.

TIGRFAMs

TIGR00656; asp_kin_monofn; 1.
TIGR00657; asp_kinases; 1.

PROSITE

PS00324; ASPARTOKINASE; 1.

BLOCKS

Q8RQN1.

Genome annotation databases

1034892; -.

BA000035_GR; CE0220.

cef:CE0220; -.

fig|196164.1.peg.220; -.

Phylogenomic databases

HOGENOM

Q8RQN1; -.

Genome annotation databases

CMR

Q8RQN1; CE0220.

Other

ProtoNet

Q8RQN1.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative initiation; Amino-acid biosynthesis; Complete proteome; Diaminopimelate biosynthesis; Kinase; Lysine biosynthesis; Repeat; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 421`	`421`	`Aspartokinase.`	`PRO_0000002375`
`DOMAIN`	`266 341`	`76`	`ACT 1.`
`DOMAIN`	`348 413`	`66`	`ACT 2.`
`VAR_SEQ`	`1 249`		`Missing (in isoform Beta).`	`VSP_018656`

Sequence information

Length: 421 AA [This is the length of the unprocessed precursor]

Molecular weight: 44794 Da [This is the MW of the unprocessed precursor]

CRC64: F0D638306983CB5F [This is a checksum on the sequence]

        10         20         30         40         50         60 
MALVVQKYGG SSLESAERIR NVAERIVATK KAGNDVVVVC SAMGDTTDEL LDLAAAVNPV 

        70         80         90        100        110        120 
PPAREMDMLL TAGERISNAL VAMAIESLGA EAQSFTGSQA GVLTTERHGN ARIVDVTPGR 

       130        140        150        160        170        180 
VREALDEGKI CIVAGFQGVN KETRDVTTLG RGGSDTTAVA LAAALGADVC EIYSDVDGVY 

       190        200        210        220        230        240 
TADPRIVPNA QKLERLSFEE MLELAAVGSK ILVLRSVEYA RAFNVPMRVR SSYSNDPGTL 

       250        260        270        280        290        300 
IAGSMEDIPM EEAVLTGVAT DKSEAKVTVL GIPDKPGEAA KVFRALADAE INIDMVLQNV 

       310        320        330        340        350        360 
SSVEDGTTDI TFTCPRSDGP RAMELLKKMQ QQGDWTNVLY DDQVGKVSLV GAGMKSHPGV 

       370        380        390        400        410        420 
TAEFMEALRD VNVNVELIST SEIRISVLIR EDDLDKSAKA LHEKFQLGGD EEATVYAGTG 


R

Q8RQN1 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools