NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395;
Nonaka G., Kimura E., Kawahara Y., Sugimoto S.;
"Corynebacterium efficiens icd gene for isocitrate dehydrogenase, complete cds.";
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.

[2]

NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395;
DOI=10.1101/gr.1285603; PubMed=12840036 [NCBI, ExPASy, EBI, Israel, Japan]
Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.;
"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens.";
Genome Res. 13:1572-1579(2003).

Comments

CATALYTIC ACTIVITY: Isocitrate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH.
CATALYTIC ACTIVITY: Oxalosuccinate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH.
COFACTOR: Binds 1 magnesium or manganese ion per subunit (By similarity).
ENZYME REGULATION: Weakly inhibited by oxaloacetate, 2-oxoglutarate, and citrate. Severely inhibited by oxaloacetate plus glyoxylate (By similarity).
SUBUNIT: Monomer (By similarity).
SIMILARITY: Belongs to the monomeric-type IDH family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB083179; BAB88832.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BA000035; BAC17492.1; ALT_INIT; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_737292.1; -.

3D structure databases

HSSP

P16100; 1ITW. [HSSP ENTRY / PDB]

SMR

Q8RQL9; 2-727.

ModBase

Q8RQL9.

Enzyme and pathway databases

BioCyc

CEFF196164:CE0682-MON; -.

Family and domain databases

InterPro

IPR004436; IsoCit_DHase_NAD_mono.
Graphical view of domain structure.

Pfam

PF03971; IDH; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF009407; IDH_monmr; 1.

TIGRFAMs

TIGR00178; monomer_idh; 1.

BLOCKS

Q8RQL9.

Genome annotation databases

1033167; -.

BA000035_GR; CE0682.

cef:CE0682; -.

fig|196164.1.peg.682; -.

Phylogenomic databases

HOGENOM

Q8RQL9; -.

Genome annotation databases

CMR

Q8RQL9; CE0682.

Other

ProtoNet

Q8RQL9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Glyoxylate bypass; Magnesium; Metal-binding; NADP; Oxidoreductase; Phosphoprotein; Tricarboxylic acid cycle.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 729`	`729`	`Isocitrate dehydrogenase [NADP].`	`PRO_0000083594`
`NP_BIND`	`80 85`	`6`	`NADP (By similarity).`
`NP_BIND`	`571 572`	`2`	`NADP (By similarity).`
`NP_BIND`	`587 589`	`3`	`NADP (By similarity).`
`REGION`	`121 128`	`8`	`Substrate binding (By similarity).`
`METAL`	`337 337`		`Magnesium or manganese (By similarity).`
`METAL`	`535 535`		`Magnesium or manganese (By similarity).`
`BINDING`	`124 124`		`NADP (By similarity).`
`BINDING`	`134 134`		`Substrate (By similarity).`
`BINDING`	`534 534`		`Substrate (By similarity).`
`BINDING`	`576 576`		`NADP (By similarity).`
`BINDING`	`636 636`		`NADP (By similarity).`
`SITE`	`244 244`	`1`	`Critical for catalysis (By similarity).`
`SITE`	`407 407`	`1`	`Critical for catalysis (By similarity).`

Sequence information

Length: 729 AA [This is the length of the unprocessed precursor]

Molecular weight: 79285 Da [This is the MW of the unprocessed precursor]

CRC64: 84BCDECA8687627B [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAKIIWTRTD EAPLLATYSL KPVVEAFAAT AGIEVETRDI SLAGRILAQF ADQLPEEQKV 

        70         80         90        100        110        120 
SDALAELGEL AKTPEANIIK LPNISASVPQ LKAAVKELQE QGYDLPEYED AKDRYAAVIG 

       130        140        150        160        170        180 
SNVNPVLREG NSDRRAPVAV KNFVKKFPHR MGEWSADSKT NVATMGADDF RSNEKSVIMD 

       190        200        210        220        230        240 
EADTVVIKHV AADGTETVLK DSLPLLKGEV IDGTFISAKA LDAFLLDQVK RAKEEGILFS 

       250        260        270        280        290        300 
AHMKATMMKV SDPIIFGHIV RAYFADVYAQ YGEQLLAAGL NGENGLAAIY AGLDKLDNGA 

       310        320        330        340        350        360 
EIKAAFDKGL EEGPDLAMVN SAKGITNLHV PSDVIIDASM PAMIRTSGKM WNKDDQTQDA 

       370        380        390        400        410        420 
LAVIPDSSYA GVYQTVIEDC RKNGAFDPTT MGTVPNVGLM AQKAEEYGSH DKTFRIEADG 

       430        440        450        460        470        480 
KVQVVASNGD VLIEHDVEKG DIWRACQTKD APIQDWVKLA VNRARLSGMP AVFWLDPARA 

       490        500        510        520        530        540 
HDRNLTTLVE KYLADHDTEG LDIQILSPVE ATQHAIDRIR RGEDTISVTG NVLRDYNTDL 

       550        560        570        580        590        600 
FPILELGTSA KMLSVVPLMA GGGLFETGAG GSAPKHVQQV IEENHLRWDS LGEFLALAES 

       610        620        630        640        650        660 
FRHELNTRNN TKAGVLADAL DRATEKLLNE EKSPSRKVGE IDNRGSHFWL ATYWADELAN 

       670        680        690        700        710        720 
QTEDAELAET FAPVAEALNN QAADIDAALI GEQGKPVDLG GYYAPSDEKT SAIMRPVAAF 


NEIIDSLKK

Q8RQL9 in FASTA format

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View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools