Polynucleotide 5'-triphosphatase
     (EC 3.1.3.33)
     (mRNA 5'-triphosphatase)
     (TPase)
mRNA guanylyltransferase
     (EC 2.7.7.50)
     (GTP--RNA guanylyltransferase)
     (GTase)

Gene name

OrderedLocusNames:

CPXV118

From

Cowpox virus (strain Brighton Red) (CPV)

[TaxID: 265872]

Taxonomy

Viruses; dsDNA viruses, no RNA stage; Poxviridae; Chordopoxvirinae; Orthopoxvirus.

Virus hosts

Bos taurus (Bovine) [TaxID: 9913]
Felis silvestris catus (Cat) [TaxID: 9685]
Homo sapiens (Human) [TaxID: 9606]
Loxodonta africana (African elephant) [TaxID: 9785]
Microtus agrestis (Short-tailed field vole) [TaxID: 29092]
Mus musculus (Mouse) [TaxID: 10090]
Myodes glareolus (Bank vole) (Clethrionomys glareolus) [TaxID: 447135]

Protein existence

3: Inferred from homology;

References

[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Dietrich F.S., Ray C.A., Sharma D.A., Allen A., Pickup D.J.; Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the first two reactions in the mRNA cap formation pathway.
CATALYTIC ACTIVITY: A 5'-phosphopolynucleotide + H₂O = a polynucleotide + phosphate.
CATALYTIC ACTIVITY: GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
SUBUNIT: Heterodimer of a large and a small subunit (By similarity).
SIMILARITY: Belongs to the viral GTase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF482758; AAM13560.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_619902.1; -.

3D structure databases

ModBase

Q8QMV9.

Family and domain databases

InterPro

IPR004971; Pox_MCEL.
Graphical view of domain structure.

Pfam

PF03291; Pox_MCEL; 1.
Pfam graphical view of domain structure.

BLOCKS

Q8QMV9.

Genome annotation databases

GeneID

1485995; -.

Other

ProtoNet

Q8QMV9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Hydrolase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotidyltransferase; Transferase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 844`	`844`	`mRNA-capping enzyme large subunit.`	`PRO_0000210128`
`ACT_SITE`	`260 260`		`N6-GMP-lysine intermediate (Potential).`

Sequence information

Length: 844 AA [This is the length of the unprocessed precursor]

Molecular weight: 96703 Da [This is the MW of the unprocessed precursor]

CRC64: C760E4B77B43A3E8 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDANVVSSST IATYIDALAK NASELEQGST AYEINNELEL VFIKPPLITL TNVVNISTIQ 

        70         80         90        100        110        120 
ESFIRFTVTN KEGVKIRTKI PLSKVHGLDV KNVQLVDAID NIVWEKKSLV TENRLHKECL 

       130        140        150        160        170        180 
LRLSTEERHI FLDYKKYGSS IRLELVNLIQ AKTKNFTIDF KLKYFLGSGA QSKSSLLHAI 

       190        200        210        220        230        240 
NHPKSRPNTS LEIEFTPRDN EKVPYDELIK ELTTLSRHIF MASPENVILS PPINPPIKTF 

       250        260        270        280        290        300 
MLPKQDIVGL DLENLYAVTK TDGIPITIRV TSKGLYCYFT HLGYIIRYPV KRIIDSEVVV 

       310        320        330        340        350        360 
FGEAVKDKNW TVYLIKLIEP VNAISDRLEE SKYVESKLVD ICDRIVFKSK KYEGPFTTTS 

       370        380        390        400        410        420 
EVVDMLSTYL PKQPEGVILF YSKGPKSNID FKIKKENTID QTANVVFRYM SSEPIIFGES 

       430        440        450        460        470        480 
SIFIEYKKFT NDKGFPKEYG SGKIVLYNGV NYLNNIYCLE YINTHNEVGI KSVVVPIKFI 

       490        500        510        520        530        540 
AEFLVNGEIL KPRIDKTMKY INSEDYYGNQ HNVIVEHLRD QSIKIGDVFN EDKLSDVGHQ 

       550        560        570        580        590        600 
YANNDKFRLN PEVSYFTNKR TRGPLGILSN YVKTLLISMY CSKTFLDDSN KRKVLAIDFG 

       610        620        630        640        650        660 
NGADLEKYFY GEIALLVATD PDADAIARGN ERYNKLNSGI KTKYYKFDYI QETIRSDTFV 

       670        680        690        700        710        720 
SSVREVFYFG KFNIIDWQFA IHYSFHPRHY ATVMNNLSEL TASGGKVLIT TMDGDKLSKL 

       730        740        750        760        770        780 
TDKKTFIIHK NLPSSENYMS VEKIADDRIL VYNPSTMSTP MTEYIIKKND IVRVFNEYGF 

       790        800        810        820        830        840 
VLVDNIDFAT IIERSKKFIN GASTMEDRPS TRNFFELNRG AIKCEGLDVE DLLSYYVVYV 


FSKR

Q8QMV9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools