ID   DP2L_METMA              Reviewed;        1152 AA.
AC   Q8PXH2;
DT   19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2002, sequence version 1.
DT   22-JUL-2008, entry version 35.
DE   RecName: Full=DNA polymerase II large subunit;
DE            Short=Pol II;
DE            EC=2.7.7.7;
GN   Name=polC; OrderedLocusNames=MM_1246;
OS   Methanosarcina mazei (Methanosarcina frisia).
OC   Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales;
OC   Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=2209;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88;
RX   MEDLINE=22120827; PubMed=12125824;
RA   Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA   Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA   Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA   Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA   Fritz H.-J., Gottschalk G.;
RT   "The genome of Methanosarcina mazei: evidence for lateral gene
RT   transfer between Bacteria and Archaea.";
RL   J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
CC   -!- FUNCTION: Possesses two activities: a DNA synthesis (polymerase)
CC       and an exonucleolytic activity that degrades single stranded DNA
CC       in the 3'- to 5'-direction. Has a template-primer preference which
CC       is characteristic of a replicative DNA polymerase (By similarity).
CC   -!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) =
CC       diphosphate + DNA(n+1).
CC   -!- CATALYTIC ACTIVITY: Degradation of single-stranded DNA. It acts
CC       progressively in a 3'- to 5'-direction, releasing nucleoside 5'-
CC       phosphates.
CC   -!- SUBUNIT: Heterodimer of a large subunit and a small subunit (By
CC       similarity).
CC   -!- SIMILARITY: Belongs to the archaeal DNA polymerase II family.
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DR   EMBL; AE008384; AAM30942.1; -; Genomic_DNA.
DR   RefSeq; NP_633270.1; -.
DR   GeneID; 1479588; -.
DR   GenomeReviews; AE008384_GR; MM_1246.
DR   KEGG; mma:MM_1246; -.
DR   NMPDR; fig|192952.1.peg.1246; -.
DR   HOGENOM; Q8PXH2; -.
DR   BioCyc; MMAZ192952:MM1246-MON; -.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:HAMAP.
DR   GO; GO:0003677; F:DNA binding; IEA:HAMAP.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:HAMAP.
DR   GO; GO:0000738; P:DNA catabolic process, exonucleolytic; IEA:HAMAP.
DR   GO; GO:0006261; P:DNA-dependent DNA replication; IEA:HAMAP.
DR   HAMAP; MF_00324; -; 1.
DR   InterPro; IPR004475; PolC_DP2.
DR   InterPro; IPR016033; PolC_DP2_N.
DR   Pfam; PF03833; PolC_DP2; 1.
DR   PIRSF; PIRSF016275; PolC_DP2; 1.
DR   TIGRFAMs; TIGR00354; polC; 1.
PE   3: Inferred from homology;
KW   Complete proteome; DNA replication; DNA-binding;
KW   DNA-directed DNA polymerase; Exonuclease; Hydrolase;
KW   Multifunctional enzyme; Nuclease; Nucleotidyltransferase; Transferase.
FT   CHAIN         1   1152       DNA polymerase II large subunit.
FT                                /FTId=PRO_0000152577.
SQ   SEQUENCE   1152 AA;  128786 MW;  48FDEEBAB8546737 CRC64;
     MGETIASEEM HRYFDGLEAR LKEAIEIANR ARARGGDPRP VVEIPLAKDL ADRVENLIGV
     KGVAEKIREL EARMSREEAA LEIGKQVAEG VVGSFPSKKD AVEAAIRVSM AVLTEGVVAA
     PIEGIDKVDL GKNDDGSQYI RIFYSGPIRS AGGTAQALSV LVGDYVRRGI GIDRYKPREE
     EVERYVEEIL LYKRVASLQY TPSEDEIRLI VRNCPVCIDG DPTEEAEVEG HRDLERIGTN
     RVRGGMCLVL AEGLALKAPK VKKHVNKLKM DGWDWLETLI GGAKSGESDE DEQKNKIKPK
     DKYIRDLIAG RPVFSHPSRP GGFRLRYGRS RNTSFASAGI NPAGMVLLDD FITNGTQLKI
     ERPGKAAAMS AVDSIEGPTV RLFSGDLIRV DDIKEAYEVR QQVEVIVDIG EILINYGDFL
     ENNHPLMPSP YVFEWWIYDY ESVCSEKILE KDLKNPSASL ALKLAEKYNV PLHPKFTYLW
     HDINRNEFEA LRKFVAEKGT FLEGEGEGEG EGILKLPLED SVKEGIKPVL EKLLVLHKVK
     EGGIFVEEAL PFILCLGLDR SLKEKVSMPD TGDMVEAAGI LSGFKVYPKA PSRIGARMGR
     PEKSDLRKMS PAAQVLFPIS NAGGMTRNLV SASDYTSCMN AKIGEIEVEL GLRECPACGK
     ESYFWRCECG EFTNPKLSCP RCKIDVRGAE TCPKCGRKPT SVANVKLDFR PIYKQAFENV
     GERERMDIIK GVKRLMNGQM TPEPLEKGIL RAKHDVYIFK DGTVRYDMSD IPLTHIRADE
     LGITAARLRE LDYKEDIYGK PLERDDQVVC LKVQDLLLSY DGAEYMLRTA KYVDELLVKY
     YKVEPYYNAE TIQDLVGVLM IGLAPHTSAG VLGRLIGFTK ASVGYAHPFF HASKRRNCDG
     DEDCVMLLMD GILNFSRSYL PEKRGGKMDA PLVLTTRIDP KEVDKEAHNI DLLARYPLEF
     YRATQEIKNP TEIESIMDLV SSRLGKPDQY EHFMFTHDTS NIAAGPLKSS YKTLGSMIEK
     MEAQLSLAGK IRAVDAPDVA ERVLKSHFLP DLIGNLRSFS RQRMRCIKCG EKFRRPPLTG
     ACPKCGGNVV LTVHEGAVRK YLEISKEIGE RYGVSSYTRQ RIELLDYDIC SLFENHKVKQ
     LGLSDFMSGS AR
//