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UniProtKB/Swiss-Prot entry Q8PU28

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name TRM1_METMA
Primary accession number Q8PU28
Secondary accession numbers None
Integrated into Swiss-Prot on October 10, 2002
Sequence was last modified on October 10, 2002 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 35)
Name and origin of the protein
Protein name N(2),N(2)-dimethylguanosine tRNA methyltransferase
Synonyms EC 2.1.1.32
tRNA(guanine-26,N(2)-N(2)) methyltransferase
tRNA 2,2-dimethylguanosine-26 methyltransferase
tRNA(m(2,2)G26)dimethyltransferase
Gene name
Name: trm1
OrderedLocusNames: MM_2528
From
Methanosarcina mazei (Methanosarcina frisia) [TaxID: 2209] [HAMAP proteome]
Taxonomy Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales; Methanosarcinaceae; Methanosarcina.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88;
PubMed=12125824 [NCBI, ExPASy, EBI, Israel, Japan]
Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.;
"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea.";
J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
Comments
  • FUNCTION: Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups (By similarity).
  • CATALYTIC ACTIVITY: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N2-methylguanine.
  • SIMILARITY: Belongs to the TRM1 family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE008384; AAM32224.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_634552.1; -.
3D structure databases
ModBase Q8PU28.
Enzyme and pathway databases
BioCyc MMAZ192952:MM2528-MON; -.
Ontologies
GO
GO:0003723; Molecular function: RNA binding (inferred from electronic annotation from InterPro).
GO:0004809; Molecular function: tRNA (guanine-N2-)-methyltransferase activity (inferred from electronic annotation from HAMAP).
GO:0006400; Biological process: tRNA modification (inferred from electronic annotation from HAMAP).
QuickGo view.
Family and domain databases
HAMAP MF_00290; -; 1.
PBIL [Tree]
InterPro IPR002905; TRM_MeTrfase.
Graphical view of domain structure.
PANTHER PTHR10631; TRM_mtfrase; 1.
Pfam PF02005; TRM; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00308; TRM1; 1.
ProtoNet Q8PU28.
Genome annotation databases
GeneID 1480870; -.
GenomeReviews AE008384_GR; MM_2528.
KEGG mma:MM_2528; -.
NMPDR fig|192952.1.peg.2528; -.
Phylogenomic databases
HOGENOM Q8PU28; -.
Genome annotation databases
CMR Q8PU28; MM_2528.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Methyltransferase; S-adenosyl-L-methionine; Transferase; tRNA processing.
Features
SEVIEWER logo Feature table viewer
KeyFrom To Length Description FTId
CHAIN   1   388  388     N(2),N(2)-dimethylguanosine tRNA methyltransferase. PRO_0000147684
Sequence information
Length: 388 AA [This is the length of the unprocessed precursor] Molecular weight: 42784 Da [This is the MW of the unprocessed precursor] CRC64: F3796A5EC7820DD3 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MICRTIVEGT TKISVPVPPP DANFPPSAAP VFYNPEMELN RDINVAATAA FVERLLSKKD 

        70         80         90        100        110        120 
ILREEIRYVD AFSASGIRGL RIAGEVGIHS TMNDWSHEAF ELIKENIKIN GLEEKAQATR 

       130        140        150        160        170        180 
RNANVLLHEQ RFHIVDVDPF GTPSPYLDAA ASSAYSMLSV TATDTAPLCG AHLNSGIRKY 

       190        200        210        220        230        240 
ASVPLNTEYH SEMGLRVLLG ACARELAKHE KGMLPLLSHV TRHYVRTYLE VLPGSRKADK 

       250        260        270        280        290        300 
TLKSMGFVAH CPRCGFRKPV YGLAVHIEKE CPECGGLTKI AGPLWLGPYR EPEFCNEVIS 

       310        320        330        340        350        360 
ELEAHPLNTK EKVRKIITFC RDELDIPMFY DQHVICKELG ASATGIESLI EALRAGGFEA 

       370        380 
SRTHFTGTSF KTDAPIAEIK KIILALSG
Q8PU28 in FASTA format

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