ID   IDH_STAAW               Reviewed;         422 AA.
AC   Q8NW61;
DT   28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-NOV-2008, entry version 36.
DE   RecName: Full=Isocitrate dehydrogenase [NADP];
DE            Short=IDH;
DE            EC=1.1.1.42;
DE   AltName: Full=Oxalosuccinate decarboxylase;
DE   AltName: Full=NADP(+)-specific ICDH;
DE   AltName: Full=IDP;
GN   Name=icd; Synonyms=citC; OrderedLocusNames=MW1638;
OS   Staphylococcus aureus (strain MW2).
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX   NCBI_TaxID=196620;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22040717; PubMed=12044378; DOI=10.1016/S0140-6736(02)08713-5;
RA   Baba T., Takeuchi F., Kuroda M., Yuzawa H., Aoki K., Oguchi A.,
RA   Nagai Y., Iwama N., Asano K., Naimi T., Kuroda H., Cui L.,
RA   Yamamoto K., Hiramatsu K.;
RT   "Genome and virulence determinants of high virulence community-
RT   acquired MRSA.";
RL   Lancet 359:1819-1827(2002).
CC   -!- CATALYTIC ACTIVITY: Isocitrate + NADP(+) = 2-oxoglutarate + CO(2)
CC       + NADPH.
CC   -!- CATALYTIC ACTIVITY: Oxalosuccinate + NADP(+) = 2-oxoglutarate +
CC       CO(2) + NADPH.
CC   -!- COFACTOR: Binds 1 magnesium or manganese ion per subunit (By
CC       similarity).
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the isocitrate and isopropylmalate
CC       dehydrogenases family.
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DR   EMBL; BA000033; BAB95503.1; -; Genomic_DNA.
DR   RefSeq; NP_646455.1; -.
DR   HSSP; P39126; 1HQS.
DR   SMR; Q8NW61; 3-421.
DR   GeneID; 1003750; -.
DR   GenomeReviews; BA000033_GR; MW1638.
DR   KEGG; sam:MW1638; -.
DR   HOGENOM; Q8NW61; -.
DR   BioCyc; SAUR196620:MW1638-MON; -.
DR   GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   InterPro; IPR004439; IsoCit_DHase_NADP_prok.
DR   InterPro; IPR001804; IsoCit_IM_DHase.
DR   Gene3D; G3DSA:3.40.718.10; IDH_IMDH; 1.
DR   PANTHER; PTHR11835; IDH_IMDH_dimeric; 1.
DR   PANTHER; PTHR11835:SF1; NADP_IDH_prok; 1.
DR   Pfam; PF00180; Iso_dh; 1.
DR   TIGRFAMs; TIGR00183; prok_nadp_idh; 1.
DR   PROSITE; PS00470; IDH_IMDH; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Glyoxylate bypass; Magnesium; Manganese;
KW   Metal-binding; NADP; Oxidoreductase; Tricarboxylic acid cycle.
FT   CHAIN         1    422       Isocitrate dehydrogenase [NADP].
FT                                /FTId=PRO_0000083564.
FT   NP_BIND     344    350       NADP (By similarity).
FT   METAL       310    310       Magnesium or manganese (By similarity).
FT   BINDING      94     94       NADP (By similarity).
FT   BINDING     103    103       Substrate (By similarity).
FT   BINDING     105    105       Substrate (By similarity).
FT   BINDING     109    109       Substrate (By similarity).
FT   BINDING     119    119       Substrate (By similarity).
FT   BINDING     143    143       Substrate (By similarity).
FT   BINDING     357    357       NADP; via amide nitrogen and carbonyl
FT                                oxygen (By similarity).
FT   BINDING     396    396       NADP (By similarity).
FT   BINDING     400    400       NADP (By similarity).
FT   SITE        150    150       Critical for catalysis (By similarity).
FT   SITE        220    220       Critical for catalysis (By similarity).
SQ   SEQUENCE   422 AA;  46437 MW;  11AE808D40093BC7 CRC64;
     MTAEKITQGT EGLNVPNEPI IPFIIGDGIG PDIWKAASRV IDAAVEKAYN GEKRIEWKEV
     LAGQKAFDTT GEWLPQETLD TIKEYLIAVK GPLTTPIGGG IRSLNVALRQ ELDLFTCLRP
     VRWFKGVPSP VKRPQDVDMV IFRENTEDIY AGIEFKEGTT EVKKVIDFLQ NEMGATNIRF
     PETSGIGIKP VSKEGTERLV RAAIQYAIDN NRKSVTLVHK GNIMKFTEGS FKQWGYDLAL
     SEFGDQVFTW QQYDEIVEKE GRDAANAAQE KAEKEGKIII KDSIADIFLQ QILTRPAEHD
     VVATMNLNGD YISDALAAQV GGIGIAPGAN INYETGHAIF EATHGTAPKY AGLNKVNPSS
     VILSSVLMLE HLGWQEAADK ITDSIEDTIA SKVVTYDFAR LMDGAEEVST SAFADELIKN
     LK
//