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UniProtKB/Swiss-Prot entry Q8MT36


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MES4_DROME
Primary accession number Q8MT36
Secondary accession numbers Q1LZ05 Q9VAY5
Integrated into Swiss-Prot on March 28, 2003
Sequence was last modified on March 28, 2003 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 56)
Name and origin of the protein
Protein name Probable histone-lysine N-methyltransferase Mes-4
Synonyms EC 2.1.1.43
Maternal-effect sterile 4 homolog
Gene name
Name: Mes-4
ORFNames: CG4976
From
Drosophila melanogaster (Fruit fly) [TaxID: 7227] 
Taxonomy Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Berkeley;
DOI=10.1126/science.287.5461.2185; PubMed=10731132 [NCBI, ExPASy, EBI, Israel, Japan]
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
"The genome sequence of Drosophila melanogaster.";
Science 287:2185-2195(2000).
[2]
GENOME REANNOTATION.
PubMed=12537572 [NCBI, ExPASy, EBI, Israel, Japan]
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.";
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=Berkeley;
TISSUE=Embryo;
PubMed=12537569 [NCBI, ExPASy, EBI, Israel, Japan]
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.;
"A Drosophila full-length cDNA resource.";
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=Berkeley;
TISSUE=Embryo;
Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.;
Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-157; SER-158; SER-169; SER-433; SER-579; SER-581; SER-588; SER-604; THR-607; SER-721; THR-723 AND SER-834, AND MASS SPECTROMETRY.
TISSUE=Embryo;
DOI=10.1021/pr700696a; PubMed=18327897 [NCBI, ExPASy, EBI, Israel, Japan]
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
"Phosphoproteome analysis of Drosophila melanogaster embryos.";
J. Proteome Res. 7:1675-1682(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE014297; AAF56762.2; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY118404; AAM48433.1; ALT_FRAME; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT025221; ABF17912.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_733239.1; -.
UniGene Dm.23351
3D structure databases
HSSP Q14839; 1MM2. [HSSP ENTRY / PDB]
ModBase Q8MT36.
Protein-protein interaction databases
DIP DIP:23366N; -.
IntAct Q8MT36; -.
Enzyme and pathway databases
BioCyc DMEL-XXX-02:DMEL-XXX-02-013791-MON; -.
Organism-specific databases
FlyBase FBgn0039559; Mes-4.
Gene expression databases
ArrayExpress Q8MT36; -.
GermOnline CG4976; Drosophila melanogaster.
Ontologies
GO
GO:0000228; Cellular component: nuclear chromosome (inferred from sequence or structural similarity from UniProtKB).
GO:0018024; Molecular function: histone-lysine N-methyltransferase activity (inferred from electronic annotation from InterPro).
GO:0005515; Molecular function: protein binding (inferred from electronic annotation from InterPro).
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from InterPro).
GO:0016568; Biological process: chromatin modification (inferred from electronic annotation from UniProtKB-KW).
GO:0016458; Biological process: gene silencing (inferred from sequence or structural similarity from UniProtKB).
GO:0018992; Biological process: germ-line sex determination (inferred from sequence or structural similarity from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR006560; AWS.
IPR003616; Post-SET_Zn_bd.
IPR000313; PWWP.
IPR001214; SET.
IPR001965; Znf_PHD.
IPR001841; Znf_RING.
Graphical view of domain structure.
Pfam PF00628; PHD; 2.
PF00855; PWWP; 2.
PF00856; SET; 1.
Pfam graphical view of domain structure.
SMART SM00570; AWS; 1.
SM00249; PHD; 3.
SM00508; PostSET; 1.
SM00293; PWWP; 2.
SM00184; RING; 2.
SM00317; SET; 1.
SMART graphical view of domain structure.
PROSITE PS51215; AWS; 1.
PS50868; POST_SET; 1.
PS50812; PWWP; 2.
PS50280; SET; 1.
PS01359; ZF_PHD_1; 1.
PS50016; ZF_PHD_2; 1.
PROSITE graphical view of domain structure (profiles).
ProtoNet Q8MT36.
Genome annotation databases
Ensembl CG4976; Drosophila melanogaster. [Contig view]
GeneID 43351; -.
KEGG dme:Dmel_CG4976; -.
NMPDR fig|7227.3.peg.15057; -.
Phylogenomic databases
HOGENOM Q8MT36; -.
Other
NextBio 833495; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Chromatin regulator; Complete proteome; Metal-binding; Methyltransferase; Nucleus; Phosphoprotein; Repeat; Transferase; Zinc; Zinc-finger.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   1427  1427     Probable histone-lysine N-methyltransferase Mes-4. PRO_0000186084
DOMAIN   395    468  74     PWWP 1. 
DOMAIN   1049   1111  63     PWWP 2. 
DOMAIN   1182   1232  51     AWS. 
DOMAIN   1233   1355  123     SET. 
DOMAIN   1359   1375  17     Post-SET. 
ZN_FING   777    862  86     PHD-type 1. 
ZN_FING   864    932  69     PHD-type 2. 
ZN_FING   999   1044  46     PHD-type 3. 
COMPBIAS   95    100  6     Poly-Gln. 
COMPBIAS   174    181  8     Poly-Gly. 
MOD_RES   157    157        Phosphoserine. 
MOD_RES   158    158        Phosphoserine. 
MOD_RES   169    169        Phosphoserine. 
MOD_RES   433    433        Phosphoserine. 
MOD_RES   579    579        Phosphoserine. 
MOD_RES   581    581        Phosphoserine. 
MOD_RES   588    588        Phosphoserine. 
MOD_RES   604    604        Phosphoserine. 
MOD_RES   607    607        Phosphothreonine. 
MOD_RES   721    721        Phosphoserine. 
MOD_RES   723    723        Phosphothreonine. 
MOD_RES   834    834        Phosphoserine. 
Sequence information
Length: 1427 AA [This is the length of the unprocessed precursor] Molecular weight: 159028 Da [This is the MW of the unprocessed precursor] CRC64: FB6EFD62E814BEF3 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKLSTDAHSE IEGDAAHGNV LCNSASDSLT ATDEVAAGND ESVATEGDDV EIPRDTNNST 

        70         80         90        100        110        120 
PVRLLDKPGQ NPVQNGAQPA AEESELESQR QTPVQKQQQQ RVSMVNRKRD LINLQSALSP 

       130        140        150        160        170        180 
KYIGYANANS PTPLSDSDDT IRTTRRRVNQ AAALNNSSAG ETLAHDNASP RTPGGGGGGG 

       190        200        210        220        230        240 
GDDSANQLLS KTYMSPIEKL LIKNGASSPN STGFEAGSED LGIRPIVRKH VKRKMKRVPK 

       250        260        270        280        290        300 
AKVTLELDEK NQQEVDEKSV KTEPIDEEVD RTDEAPTQEA QTTAISIKSE TEAEHKAAVD 

       310        320        330        340        350        360 
VHIKQEDTIR LDIVNNPVES TSIVITEEPK DLEKSTEELA FALPLASSTE VDLKSPPDLS 

       370        380        390        400        410        420 
STALATSIKS PSSVSIDSAK GLSIVTDPGW PTYQVGDLFW GKVFSYCFWP CMVCPDPLGQ 

       430        440        450        460        470        480 
IVGNMPSHPQ RSSLDNANVP IQVHVRFFAD NGRRNWIKPE NLLTFAGLKA FDDMREELRI 

       490        500        510        520        530        540 
KHGPKSAKYR QMVPKRTKVV IWRQAIEEAQ AMTQIPYSDR LEKFYQTYEN VVTLNRQKRK 

       550        560        570        580        590        600 
RTKYMMQDTS DVGSSLYDST DNLHNKQGTQ LLAVKRERSE SPFSPAFSPV KSKNEKRAKR 

       610        620        630        640        650        660 
RKLSNGTEAD TGSNSMAVTP SQTETTVDSS AYENPEFRQL LSAVMEYVMM NRSDEKVEKV 

       670        680        690        700        710        720 
LLSVVSNIWS LKQIQLRELE RDLASGEIEE PLGSSVVGRG SGVGTIKRLS NRLMTMMVRR 

       730        740        750        760        770        780 
SMTPVVTPST TPAPSEPDRR LSEPPKTKKP VNRPIEEVIE DILQLDSKYL FRGLSREPIC 

       790        800        810        820        830        840 
KYCYQAGSDL VRCSRTCSSW LHADCLERKV TGAPMPKIGS RKALVIPPTS KSPSPDEDHV 

       850        860        870        880        890        900 
TADAKEVVAV GTSLVCHECN VGEPEGCVIC HQVESPAVPS TPRKEDSSSH TPIEDKLLTC 

       910        920        930        940        950        960 
SQPMCGKRFH TSCCKYWPQA SSSKHSARCP RHVCHTCVSD DPSGKFQQLG SSKLAKCVRC 

       970        980        990       1000       1010       1020 
PATYHQLSKC IPAGTQMLNT TNIICPRHNI AKADAHVNVL WCYICVKGGE LVCCETCPIA 

      1030       1040       1050       1060       1070       1080 
VHAHCRNIPI KTNESYICEE CESGRLPLYG EIVWAKFNNF RWWPAIILPP TEVPSNILKK 

      1090       1100       1110       1120       1130       1140 
AHGENDFVVR FFGTHDHGWI SRRRVYLYIE GDTGDGHKTK SQLFRNYTTG VEEASRFLPI 

      1150       1160       1170       1180       1190       1200 
IKARRQEQDM ERQSGNKLHP PPYVKIKTNK AVPPLRFSQN LEDLSTCNCL PVDEHPCGPE 

      1210       1220       1230       1240       1250       1260 
AGCLNRMLFN ECNPEYCKAG SLCENRMFEQ RKSPRLEVVY MNERGFGLVN REPIAVGDFV 

      1270       1280       1290       1300       1310       1320 
IEYVGEVINH AEFQRRMEQK QRDRDENYYF LGVEKDFIID AGPKGNLARF MNHSCEPNCE 

      1330       1340       1350       1360       1370       1380 
TQKWTVNCIH RVGIFAIKDI PVNSELTFNY LWDDLMNNSK KACFCGAKRC SGEIGGKLKD 

      1390       1400       1410       1420 
DAVKAHAKLK QMRRAKASAV RIHVKPKKTP KVKHISADDE PMDAKDE 

Q8MT36 in FASTA format

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BLAST logo BLAST submission on ExPASy/SIB
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