[1]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Fruit, and Leaf; Campa C., Legal L., Khounlotham M., Noirot M., de Kochko A.; "Complete cDNA sequence of a caffeic acid O-methyltransferase from Coffea canephora fruit."; Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the conversion of caffeic acid to ferulic acid and of 5-hydroxyferulic acid to sinapic acid. The resulting products may subsequently be converted to the corresponding alcohols that are incorporated into lignins.
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate.
PATHWAY: Aromatic compound metabolism; phenylpropanoid biosynthesis .
SUBUNIT: Homodimer (By similarity).
SIMILARITY: Belongs to the methyltransferase superfamily. Type 2 family. COMT subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF454631; AAN03726.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF454632; AAN03727.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P28002; 1KYZ. [HSSP ENTRY / PDB]

SMR

Q8LL87; 1-350.

ModBase

Q8LL87.

Ontologies

GO:0047763;	Molecular function: caffeate O-methyltransferase activity (inferred from electronic annotation from EC).
GO:0046983;	Molecular function: protein dimerization activity (inferred from electronic annotation from InterPro).
GO:0009809;	Biological process: lignin biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR016461; O-MeTrfase_COMT_euk.
IPR001077; O_MeTrfase_2.
IPR012967; Plant_MeTrfase_dimerisation.
IPR011991; Wing_hlx_DNA_bd.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.10.10; Wing_hlx_DNA_bd; 1.

Pfam

PF08100; Dimerisation; 1.
PF00891; Methyltransf_2; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF005739; O-mtase; 1.

ProtoNet

Q8LL87.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Lignin biosynthesis; Methyltransferase; S-adenosyl-L-methionine; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 350`	`350`	`Caffeic acid 3-O-methyltransferase.`	`PRO_0000063200`
`REGION`	`117 123`	`7`	`Substrate binding (By similarity).`
`REGION`	`149 167`	`19`	`Substrate binding (By similarity).`
`ACT_SITE`	`256 256`		`Proton acceptor (By similarity).`
`BINDING`	`195 195`		`S-adenosyl-L-methionine; via carbonyl oxygen (By similarity).`
`BINDING`	`218 218`		`S-adenosyl-L-methionine (By similarity).`
`BINDING`	`238 238`		`S-adenosyl-L-methionine (By similarity).`
`BINDING`	`239 239`		`S-adenosyl-L-methionine; via amide nitrogen (By similarity).`
`BINDING`	`252 252`		`S-adenosyl-L-methionine (By similarity).`
`CONFLICT`	`230 230`		`S -> P (in Ref. 1; AAN03726).`
`CONFLICT`	`268 268`		`K -> R (in Ref. 1; AAN03726).`

Sequence information

Length: 350 AA [This is the length of the unprocessed precursor]

Molecular weight: 38258 Da [This is the MW of the unprocessed precursor]

CRC64: D6AC10007BDF8E6B [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAEEEACLFA MSLASASVLP MVLKSAIELD LLELIAKAGP GAYVSPSELA AQLPTHNPEA 

        70         80         90        100        110        120 
PIMLDRILRL LATYSVLDCK LNNLADGGVE RLYGLAPVCK FLTKNADGVS MAPLLLMNQD 

       130        140        150        160        170        180 
KVLMESWYHL KDAVLDGGIP FNKAYGMTAF EYHGTDPRFN KVFNQGMSNH STITMKKILE 

       190        200        210        220        230        240 
VYRGFEGLKT VVDVGGGTGA TLNMIISKYP TIKGINFELP HVVEDAPSHS GVEHVGGDMF 

       250        260        270        280        290        300 
VSVPKGDAIF MKWICHDWSD DHCRKLLKNC YQALPDNGKV ILAECVLPEA PDTSLATQNV 

       310        320        330        340        350 
VHVDVVMLAH NPGGKERTEK EFEALAKGAG FKEFRKVCSA VNTWIMELCK

Q8LL87 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools