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[1]
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NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, AND PHODPHORYLATION.
TISSUE=Endosperm, and Leaf;
DOI=10.1016/S0014-5793(02)03455-5; PubMed=12387887 [NCBI, ExPASy, EBI, Israel, Japan]
Bustos D.M.,
Iglesias A.A.;
"Non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase is post-translationally phosphorylated in heterotrophic cells of wheat (Triticum aestivum).";
FEBS Lett. 530:169-173(2002).
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[2]
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SEQUENCE REVISION.
TISSUE=Leaf;
Piattoni C.V.,
Guerrero S.A.,
Bustos D.M.,
Iglesias A.A.;
Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
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[3]
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INTERACTION WITH 14-3-3 PROTEIN, SUBCELLULAR LOCATION, AND ENZYME REGULATION.
DOI=10.1104/pp.103.030981; PubMed=14681537 [NCBI, ExPASy, EBI, Israel, Japan]
Bustos D.M.,
Iglesias A.A.;
"Phosphorylated non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from heterotrophic cells of wheat interacts with 14-3-3 proteins.";
Plant Physiol. 133:2081-2088(2003).
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[4]
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3D-STRUCTURE MODELING.
DOI=10.1016/j.jmgm.2005.03.002; PubMed=15896993 [NCBI, ExPASy, EBI, Israel, Japan]
Bustos D.M.,
Iglesias A.A.;
"A model for the interaction between plant GAPN and 14-3-3zeta using protein-protein docking calculations, electrostatic potentials and kinetics.";
J. Mol. Graph. Model. 23:490-502(2005).
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- FUNCTION: Important as a means of generating NADPH for biosynthetic reactions.
- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + NADP+ + H2O = 3-phospho-D-glycerate + NADPH.
- ENZYME REGULATION: Insensitive to magnesium or calcium when dephosphorylated. When phosphorylated, 3-fold activation by magnesium or calcium, 2-fold activation by potasium, inhibited by ADP and AMP and insensitive to ATP or PPi.
- BIOPHYSICOCHEMICAL PROPERTIES:
| Kinetic parameters: |
KM=40 µM for NADP; | | KM=118 µM for D-glyceraldehyde-3-phosphate; | | Note=When the protein is phosphorylated, the affinity for substrates is similar but the Vmax is lowered; | |
- SUBUNIT: Interacts with 14-3-3 protein when phosphorylated. This interaction is released by divalent cations.
- SUBCELLULAR LOCATION: Cytoplasm.
- PTM: Phosphorylated in shoots and non-photosynthetic tissues, but not in leaves.
- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
- CAUTION: Sequences of mRNA encoded by the same gene but extracted from leaves (AF521191) or from endosperm (AF521190) differ at several prositions due to sequencing uncertainties.
- SEQUENCE CAUTION:
- Sequence=AAM77678.1; Type=Frameshift; Positions=351, 369;
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 496 AA [This is the length of the unprocessed precursor] |
Molecular weight: 53047 Da [This is the MW of the unprocessed precursor] |
CRC64: F37AB38E8561A287 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MAGTGVFADV LDGEVYKYYA DGEWRASASG KTVAIVNPTT RQTQYRVQAC TQEEVNKVMD
70 80 90 100 110 120
AAKVAQKSWA RTPLWKRAEL LHKAAAILKE HKTPIAESLV KEIAKPAKDA VSEVVRSGDL
130 140 150 160 170 180
VSYTAEEGVR ILGEGKLLVS DSFPGNERNK YCLSSKVPLG VVLAIPPFNY PVNLAVSKIG
190 200 210 220 230 240
PALIAGNSLV LKPPTQGAVA ALHMVHCFHL AGFPKGLISC VTGKGSEIGD FLTMHPGVNC
250 260 270 280 290 300
ISFTGGDTGI AISKKAGMVP LQMELGGKDA CIVLEDADLD LVAANIVKGG FSYSGQRCTA
310 320 330 340 350 360
VKVVLIMEAV ADTVVEKVNA KLAKLKVGPP EDDSDITPVV TESSANFIEG LVMDAKEKGA
370 380 390 400 410 420
TFCQEYRREG NLIWPLLLDH VRPDMRIAWE EPFGPVLPVI RINSVEEGIH HCNASNFGLQ
430 440 450 460 470 480
GCVFTRDINK AIMISDAMES GTVQINSAPA RGPDHFPFQG LKDSGIGSQG ITNSINMMTK
490
VKSTVINLPS PSYTMG
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Q8LK61 in FASTA format |
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