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UniProtKB/Swiss-Prot entry Q8LGI2


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name SCPDH_ARATH
Primary accession number Q8LGI2
Secondary accession number Q9ZR98
Integrated into Swiss-Prot on March 7, 2006
Sequence was last modified on March 7, 2006 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 33)
Name and origin of the protein
Protein name Probable mitochondrial saccharopine dehydrogenase At5g39410
Synonyms SDH
EC 1.5.1.9
Gene name
OrderedLocusNames: At5g39410
ORFNames: MUL8.9
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Columbia;
Lessard P., Petit-Jean X., Kreis M., Thomas M.;
Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1093/dnares/5.1.41; PubMed=9628582 [NCBI, ExPASy, EBI, Israel, Japan]
Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones.";
DNA Res. 5:41-54(1998).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.;
"Full-length cDNA from Arabidopsis thaliana.";
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
[4]
SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS SPECTROMETRY.
DOI=10.1016/j.phytochem.2004.03.028; PubMed=15276431 [NCBI, ExPASy, EBI, Israel, Japan]
Brugiere S., Kowalski S., Ferro M., Seigneurin-Berny D., Miras S., Salvi D., Ravanel S., d'Herin P., Garin J., Bourguignon J., Joyard J., Rolland N.;
"The hydrophobic proteome of mitochondrial membranes from Arabidopsis cell suspensions.";
Phytochemistry 65:1693-1707(2004).
[5]
IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], AND SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
DOI=10.1105/tpc.016055; PubMed=14671022 [NCBI, ExPASy, EBI, Israel, Japan]
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.;
"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins.";
Plant Cell 16:241-256(2004).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U83179; AAD09232.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB009054; BAB11014.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY084257; AAM60851.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_568564.1; -.
UniGene At.49110
3D structure databases
ModBase Q8LGI2.
Organism-specific databases
TAIR At5g39410; -.
Gene expression databases
GermOnline AT5G39410; Arabidopsis thaliana.
Ontologies
GO
GO:0031966; Cellular component: mitochondrial membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005488; Molecular function: binding (inferred from electronic annotation from InterPro).
GO:0047131; Molecular function: saccharopine dehydrogenase (NAD+, L-glutamate-forming) activity (inferred from electronic annotation from EC).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR005097; Saccharopine_DHase.
Graphical view of domain structure.
Pfam PF03435; Saccharop_dh; 1.
Pfam graphical view of domain structure.
ProtoNet Q8LGI2.
Genome annotation databases
GeneID 833937; -.
GenomeReviews BA000015_GR; AT5G39410.
KEGG ath:AT5G39410; -.
NMPDR fig|3702.1.peg.25637; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Membrane; Mitochondrion; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   454  454     Probable mitochondrial saccharopine dehydrogenase At5g39410. PRO_0000226069
CONFLICT   8     8        E -> D (in Ref. 3; AAM60851). 
CONFLICT   13    13        M -> I (in Ref. 3; AAM60851). 
CONFLICT   38    38        Missing (in Ref. 3). 
CONFLICT   136   136        N -> K (in Ref. 3; AAM60851). 
CONFLICT   403   403        V -> L (in Ref. 3; AAM60851). 
Sequence information
Length: 454 AA [This is the length of the unprocessed precursor] Molecular weight: 49681 Da [This is the MW of the unprocessed precursor] CRC64: 7020142459682B6B [This is a checksum on the sequence]
        10         20         30         40         50         60 
MNPTQKPEPV YDMVILGASG FTGKYVVREA LKFLQTPSSS PLKSLALAGR NPTRLTQSLE 

        70         80         90        100        110        120 
WAARPNPPPS SVAILTADTS DPDSLRRLCT QTKLILNCVG PFRIHGDPVV SACADSGCDY 

       130        140        150        160        170        180 
LDISGEPEFM ERMEANYHDR AEETGSLIVS ACGFDSIPAE LGLLFNAKQW VSPSVPNQIE 

       190        200        210        220        230        240 
AYLSLESDKK IAGNFGTYES AVLGVANAEK LKELRRSRPR RPRPTICGPP AKGPTLENQK 

       250        260        270        280        290        300 
TIGLWALKLP SADAVVVRRT LTTLTEKPHG LPGINESPEQ IQKREAFWSS IKPAHFGVKI 

       310        320        330        340        350        360 
TSKSLFGIFR YVTLGVSLGL LSKFSFGRWL LLKFPSVFSL GWFQKKGPSE EEVESATFKM 

       370        380        390        400        410        420 
WFIGRGYSEE SLASQGETKP DLEIITRISG PEIGYITTPI TLVQCGLIVL GQRESLVKGG 

       430        440        450 
VYTPGIVFGS TDIQQRLEDN GISFELISKI KTQG 

Q8LGI2 in FASTA format

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