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UniProtKB/Swiss-Prot entry Q8LAX0


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name HMT3_ARATH
Primary accession number Q8LAX0
Secondary accession number Q9LUI7
Integrated into Swiss-Prot on July 19, 2004
Sequence was last modified on July 19, 2004 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 36)
Name and origin of the protein
Protein name Homocysteine S-methyltransferase 3
Synonyms EC 2.1.1.10
S-methylmethionine:homocysteine methyltransferase 3
SMM:Hcy S-methyltransferase 3
AtHMT-3
Gene name
Name: HMT3
OrderedLocusNames: At3g22740
ORFNames: MWI23.11
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY, PROBABLE FUNCTION OF SMM CYCLE, BIOPHYSICOCHEMICAL PROPERTIES, AND TISSUE SPECIFICITY.
STRAIN=cv. Columbia;
TISSUE=Leaf;
PubMed=11309147 [NCBI, ExPASy, EBI, Israel, Japan]
Ranocha P., McNeil S.D., Ziemak M.J., Li C., Tarczynski M.C., Hanson A.D.;
"The S-methylmethionine cycle in angiosperms: ubiquity, antiquity and activity.";
Plant J. 25:575-584(2001).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1093/dnares/7.2.131; PubMed=10819329 [NCBI, ExPASy, EBI, Israel, Japan]
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones.";
DNA Res. 7:131-135(2000).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1071006; PubMed=11910074 [NCBI, ExPASy, EBI, Israel, Japan]
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.;
"Functional annotation of a full-length Arabidopsis cDNA collection.";
Science 296:141-145(2002).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.;
"Full-length cDNA from Arabidopsis thaliana.";
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF297394; AAG10301.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB022223; BAB01249.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK118021; BAC42654.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT005318; AAO63382.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY087554; AAM65096.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_566715.1; -.
UniGene At.6305
3D structure databases
ModBase Q8LAX0.
Organism-specific databases
TAIR At3g22740; -.
Gene expression databases
ArrayExpress Q8LAX0; -.
GermOnline AT3G22740; Arabidopsis thaliana.
Ontologies
GO
GO:0008898; Molecular function: homocysteine S-methyltransferase activity (inferred from electronic annotation from InterPro).
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0009086; Biological process: methionine biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR003726; S_MeTrfase.
Graphical view of domain structure.
Gene3D G3DSA:3.20.20.330; S_methyl_trans; 1.
Pfam PF02574; S-methyl_trans; 1.
Pfam graphical view of domain structure.
PROSITE PS50970; HCY; 1.
PROSITE graphical view of domain structure (profiles).
ProtoNet Q8LAX0.
Genome annotation databases
GeneID 821845; -.
GenomeReviews BA000014_GR; AT3G22740.
KEGG ath:AT3G22740; -.
NMPDR fig|3702.1.peg.14522; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amino-acid biosynthesis; Complete proteome; Metal-binding; Methionine biosynthesis; Methyltransferase; S-adenosyl-L-methionine; Transferase; Zinc.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   347  347     Homocysteine S-methyltransferase 3. PRO_0000114613
DOMAIN   12   333  322     Hcy-binding. 
METAL   251   251        Zinc (Potential). 
METAL   318   318        Zinc (Potential). 
METAL   319   319        Zinc (Potential). 
CONFLICT   24    24        A -> E (in Ref. 5; AAM65096). 
CONFLICT   45    45        L -> I (in Ref. 5; AAM65096). 
CONFLICT   117   117        C -> W (in Ref. 5; AAM65096). 
Sequence information
Length: 347 AA [This is the length of the unprocessed precursor] Molecular weight: 37882 Da [This is the MW of the unprocessed precursor] CRC64: 45CDC34973F820CC [This is a checksum on the sequence]
        10         20         30         40         50         60 
MGSFVKEETS SLMTDFLEKC GGYAVVDGGF ATELQRHGAD INDPLWSAKC LITSPHLVTK 

        70         80         90        100        110        120 
VHLDYLESGA NIIITASYQA TIQGFVAKGL SVGEAENLLR RSVEITYEAR EIFYNRCTKG 

       130        140        150        160        170        180 
SWDFAYAGKA SRRPILVAAS VGSYGAYLAD GSEYSGIYGD SVSKETLKDF HRRRVQILAK 

       190        200        210        220        230        240 
SGADLIAFET IPNKLEAEAY ADLLEEEDID IPAWFSFTSK DGVSVPRGDS VVECAKVADS 

       250        260        270        280        290        300 
CKNVVAIGIN CTAPRYIHAL IISLRQMTRK PIVVYPNSGE VYDGLNKKWI KSEGESEEDF 

       310        320        330        340 
VSYVSKWRDA GASLFGGCCR TTPNTIRAIA KVLSDEPSAA SKPKFGQ 

Q8LAX0 in FASTA format

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BLAST logo BLAST submission on ExPASy/SIB
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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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NPSA logo NPSA Sequence analysis tools

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