NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=A1/3;
DOI=10.1007/s00203-004-0743-8; PubMed=15609023 [NCBI, ExPASy, EBI, Israel, Japan]
Steinborn G., Hajirezaei M.-R., Hofemeister J.;
"bac genes for recombinant bacilysin and anticapsin production in Bacillus host strains.";
Arch. Microbiol. 183:71-79(2005).

Comments

FUNCTION: Catalyzes the formation of alpha-dipeptides from various L-amino acids in the presence of ATP. Probably catalyzes the ligation of L-alanine and L-anticapsin to produce the final bacilysin antibiotic (By similarity).
CATALYTIC ACTIVITY: ATP + an L-amino acid + an L-amino acid = ADP + phosphate + L-aminoacyl-L-amino acid.
COFACTOR: Magnesium or manganese (By similarity).
PATHWAY: Antibiotic biosynthesis; bacilysin biosynthesis .
SIMILARITY: Contains 1 ATP-grasp domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF396778; AAM90571.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q8KWT3.

Ontologies

GO:0005524;	Molecular function: ATP binding (inferred from electronic annotation from InterPro).
GO:0034026;	Molecular function: L-amino-acid alpha-ligase activity (inferred from electronic annotation from EC).
GO:0017000;	Biological process: antibiotic biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR011761; ATP-grasp.
Graphical view of domain structure.

PROSITE

PS50975; ATP_GRASP; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

Q8KWT3.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Antibiotic biosynthesis; ATP-binding; Ligase; Nucleotide-binding.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 472`	`472`	`Alanine-anticapsin ligase bacD.`	`PRO_0000064802`
`DOMAIN`	`142 355`	`214`	`ATP-grasp.`
`NP_BIND`	`168 235`	`68`	`ATP (By similarity).`

Sequence information

Length: 472 AA [This is the length of the unprocessed precursor]

Molecular weight: 52166 Da [This is the MW of the unprocessed precursor]

CRC64: D39D317DD624E5FC [This is a checksum on the sequence]

        10         20         30         40         50         60 
MERKTVLVIA DLGGCPPHMF YKSAAEKYNL VSFIPRPFAI TASHAALIEK YSVAVIKDKD 

        70         80         90        100        110        120 
YFKSLADFEH PDSIYWAHED HDKPEEEVVE EIVKVAGMFA VDAITTNNEL FIAPMAKACE 

       130        140        150        160        170        180 
RLGLRGAGVQ AAENARDKNK MRAAFNRAGV KSIKNKRVTT LEDFRAALQE IGTPLILKPT 

       190        200        210        220        230        240 
YLASSIGVTL IKEMETAEAE FNRVNEYLKS INVPKAVTFE APFIAEEFLQ GEYDDWYETS 

       250        260        270        280        290        300 
GYSDYISIEG IMADGEYFPV AIHDKTPQIG FTETSHITPS ILDDDAKRKI VEAAKKANEG 

       310        320        330        340        350        360 
LGLENCATHT EIKLMKNREA GLIESAARFA GWNMIPNIKK VFGVDMAQLL LDVLCFGKEA 

       370        380        390        400        410        420 
DLPKGLLEQE PCYVADCHLY PQHFKENGQL PETAVDFVIE SIDIPDGVLK GDTEIVSFSA 

       430        440        450        460        470 
AEAGTSVDLR LFEAFNSIAA FELKGSNSGD VAESIKQIQQ QAKLTAKYAL PV

Q8KWT3 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools