NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, COFACTOR, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
STRAIN=Sprague-Dawley;
TISSUE=Brain;
DOI=10.1074/jbc.M201656200; PubMed=12167635 [NCBI, ExPASy, EBI, Israel, Japan]
Failer B.U., Braun N., Zimmermann H.;
"Cloning, expression, and functional characterization of a Ca2+-dependent endoplasmic reticulum nucleoside diphosphatase.";
J. Biol. Chem. 277:36978-36986(2002).

[2]

NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Placenta;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).

Comments

FUNCTION: Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > IDP >> UTP > CDP = GTP = ITP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP.
CATALYTIC ACTIVITY: A nucleoside diphosphate + H₂O = a nucleotide + phosphate.
COFACTOR: Calcium.
BIOPHYSICOCHEMICAL PROPERTIES:

Kinetic parameters: K_M=216 µM for UDP;
SUBUNIT: Monomer (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Processed form: Secreted.
TISSUE SPECIFICITY: Detected in intestine, thymus, heart, lung, spleen, kidney, liver, testis, skeletal muscle and brain.
SIMILARITY: Belongs to the apyrase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ312207; CAC85467.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC097279; AAH97279.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_653355.1; -.

UniGene

Rn.7241

3D structure databases

Q8K4Y7; 89-403.

PTM databases

Q8K4Y7; -.

Organism-specific databases

RGD

628743; Cant1.

Gene expression databases

ArrayExpress

Q8K4Y7; -.

GermOnline

ENSRNOG00000003239; Rattus norvegicus.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005794;	Cellular component: Golgi apparatus (inferred from electronic annotation from UniProtKB-KW).
GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005509;	Molecular function: calcium ion binding (inferred from electronic annotation from InterPro).
GO:0017110;	Molecular function: nucleoside-diphosphatase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR009283; Apyrase.
Graphical view of domain structure.

PANTHER

PTHR13023; Apyrase; 1.

Pfam

PF06079; Apyrase; 1.
Pfam graphical view of domain structure.

ProtoNet

Q8K4Y7.

Genome annotation databases

Ensembl

ENSRNOG00000003239; Rattus norvegicus. [Contig view]

GeneID

246272; -.

KEGG

rno:246272; -.

Phylogenomic databases

HOVERGEN

Q8K4Y7; -.

Other

NextBio

623641; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calcium; Endoplasmic reticulum; Glycoprotein; Golgi apparatus; Hydrolase; Membrane; Metal-binding; Signal-anchor; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 403`	`403`	`Soluble calcium-activated nucleotidase 1.`	`PRO_0000209927`
`TOPO_DOM`	`1 44`	`44`	`Cytoplasmic (Potential).`
`TRANSMEM`	`45 61`	`17`	`Signal-anchor for type II membrane protein (Potential).`
`TOPO_DOM`	`62 403`	`342`	`Lumenal (Potential).`
`COMPBIAS`	`236 239`	`4`	`Poly-Thr.`
`METAL`	`170 170`		`Calcium (By similarity).`
`METAL`	`217 217`		`Calcium (By similarity).`
`METAL`	`286 286`		`Calcium (By similarity).`
`METAL`	`347 347`		`Calcium (By similarity).`
`METAL`	`398 398`		`Calcium (By similarity).`
`CARBOHYD`	`90 90`		`N-linked (GlcNAc...) (Potential).`

Sequence information

Length: 403 AA [This is the length of the unprocessed precursor]

Molecular weight: 45659 Da [This is the MW of the unprocessed precursor]

CRC64: 34340AF7917334F1 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MPIQPFDQRE WNEPMHSLRI SVGGLPVLAS MTKATDPRFR PRWRVILTSF VGAALLWLLY 

        70         80         90        100        110        120 
SHHQTPVSGR PPIHNAHNWR LRQERISQYN DTYPLSPPQR TPGGIRYRIA VIADLDTGSK 

       130        140        150        160        170        180 
AQEENTWFSY LKKGYLTLSD SGDRVSVEWD KDRGVLESHL AEKGRGMELS DLIVFNGKLY 

       190        200        210        220        230        240 
SVDDRTGVIY QIEGTKAVPW VILSDGDGAV EKGFKAEWLA VKDEHLYVGG LGKEWTTTTG 

       250        260        270        280        290        300 
EVVNENPEWV KVVGHRGSVE HENWVSSYNA LRAAAGIQPP GYLIHESACW SDTLQRWFFL 

       310        320        330        340        350        360 
PRRASHERYS EREDERKGSN LLLSAAQDFR DISVRQVGAL VPTHGFSSFK FIPNTDDQII 

       370        380        390        400 
VALKSEEDNG RIATYVMAFT LDGRFLLPET KIGSVKYEGI EFI

Q8K4Y7 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools