[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, AND TISSUE SPECIFICITY. STRAIN=C57BL/6; TISSUE=Liver; DOI=10.1021/bi0362222; PubMed=15122917 [NCBI, ExPASy, EBI, Israel, Japan] Bigonnesse F., Levesque S.A., Kukulski F., Lecka J., Robson S.C., Fernandes M.J., Sevigny J.; "Cloning and characterization of mouse nucleoside triphosphate diphosphohydrolase-8."; Biochemistry 43:5511-5519(2004).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=C57BL/6J; The mouse genome sequencing consortium; Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). STRAIN=FVB/N; TISSUE=Kidney; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).

Comments

FUNCTION: Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolyzis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP.
CATALYTIC ACTIVITY: ATP + 2 H₂O = AMP + 2 phosphate.
COFACTOR: Calcium.
BIOPHYSICOCHEMICAL PROPERTIES:

Kinetic parameters: K_M=13 µM for ATP;
K_M=41 µM for ADP;
K_M=47 µM for UTP;
K_M=171 µM for UDP;
pH dependence: Optimum pH is 5.5-8.0;
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity).
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name 1

Isoform ID Q8K0L2-1

This is the isoform sequence displayed in this entry.

Name 2

Isoform ID Q8K0L2-2

Features which should be applied to build the isoform sequence: VSP_028560, VSP_028561.
TISSUE SPECIFICITY: Expressed in liver, jejunum and kidney.
DOMAIN: The transmembranous domains are involved in regulation of enzyme activity (By similarity).
PTM: N-glycosylated (By similarity).
SIMILARITY: Belongs to the GDA1/CD39 NTPase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AY364442; AAQ84519.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AL732585; CAM17997.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AL732585; CAM17998.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC031143; AAH31143.2; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_082369.1; -.

UniGene

Mm.33403

3D structure databases

ModBase

Q8K0L2.

Organism-specific databases

MGI

MGI:1919340; Entpd8.

Gene expression databases

CleanEx

MM_ENTPD8; -.

Ontologies

GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005624;	Cellular component: membrane fraction (inferred from direct assay from MGI).
GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005524;	Molecular function: ATP binding (inferred from electronic annotation from UniProtKB-KW).
GO:0005509;	Molecular function: calcium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0017110;	Molecular function: nucleoside-diphosphatase activity (inferred from direct assay from MGI).
GO:0017111;	Molecular function: nucleoside-triphosphatase activity (inferred from direct assay from MGI).
GO:0009133;	Biological process: nucleoside diphosphate biosynthetic process (inferred from direct assay from MGI).
GO:0009124;	Biological process: nucleoside monophosphate biosynthetic process (inferred from direct assay from MGI).
QuickGo view.

Family and domain databases

InterPro

IPR000407; GDA1_CD39_NTPase.
Graphical view of domain structure.

PANTHER

PTHR11782; GDA1_CD39_NTPase; 1.

Pfam

PF01150; GDA1_CD39; 1.
Pfam graphical view of domain structure.

PROSITE

PS01238; GDA1_CD39_NTPASE; 1.

ProtoNet

Q8K0L2.

Genome annotation databases

Ensembl

ENSMUSG00000036813; Mus musculus. [Contig view]

GeneID

72090; -.

KEGG

mmu:72090; -.

Phylogenomic databases

HOVERGEN

Q8K0L2; -.

Other

NextBio

335412; -.

SOURCE

Entpd8; Mus musculus.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; ATP-binding; Calcium; Cell membrane; Glycoprotein; Hydrolase; Membrane; Nucleotide-binding; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 497`	`497`	`Ectonucleoside triphosphate diphosphohydrolase 8.`	`PRO_0000306883`
`TOPO_DOM`	`1 8`	`8`	`Cytoplasmic (Potential).`
`TRANSMEM`	`9 29`	`21`	`Potential.`
`TOPO_DOM`	`30 473`	`444`	`Extracellular (Potential).`
`TRANSMEM`	`474 494`	`21`	`Potential.`
`TOPO_DOM`	`495 497`	`3`	`Cytoplasmic (Potential).`
`CARBOHYD`	`306 306`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`365 365`		`N-linked (GlcNAc...) (Potential).`
`VAR_SEQ`	`263 265`		`Missing (in isoform 2).`	`VSP_028560`
`VAR_SEQ`	`353 389`		`Missing (in isoform 2).`	`VSP_028561`
`CONFLICT`	`437 437`		`D -> N (in Ref. 3; AAH31143).`

Sequence information

Length: 497 AA [This is the length of the unprocessed precursor]

Molecular weight: 54650 Da [This is the MW of the unprocessed precursor]

CRC64: 00DE822B6EEB1BDF [This is a checksum on the sequence]

        10         20         30         40         50         60 
MGLSWKERVF MALLGVAAAS GLTMLVLILV KAINVLLPAD TKFGIVFDAG SSHTSLFVYQ 

        70         80         90        100        110        120 
WPANKEKDTG VVSQALTCQI EGPGISSYTS DPTQAGESLK SCLEEALALI PQAQHPETPT 

       130        140        150        160        170        180 
FLGSTAGMRL LSQKNSSQAR DILAAVSQTL SKSPVDFWGA KILAGQDEGA FGWITINYVL 

       190        200        210        220        230        240 
GMLLKYSSGQ WILPEEGMLV GALDLGGAST QISFVPQGPI LDQSTQVTFR LYGANYSVYT 

       250        260        270        280        290        300 
HSYLCFGRDQ ILNRLLAKLA QDRLSSQVAP VRHPCYHSGY QAILPLSSLY DSPCIHTTDS 

       310        320        330        340        350        360 
LNHTQNLTVE GTGDPGNCVV ALRSLFNFSS CKGQKDCAFN GIYQPPVHGQ FYAFSNFYYT 

       370        380        390        400        410        420 
FHFLNLTSRQ SLNTVNDTVW KFCQKPWKLV EVSYPGQERW LRDYCASGLY ILVLLLEGYK 

       430        440        450        460        470        480 
FSEETWPNIQ FQKQAGDTDI GWTLGFMLNL TGMIPAEAPT HWRAQSYSIW TAGVVFAVLT 

       490 
LVAILGAAAI QIFWTQD

Q8K0L2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools