ID   L_ABLVH                 Reviewed;        2127 AA.
AC   Q8JTG9;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-NOV-2008, entry version 25.
DE   RecName: Full=Large structural protein;
DE            Short=Protein L;
DE   AltName: Full=Transcriptase;
DE   AltName: Full=Replicase;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=mRNA (guanine-N(7)-)-methyltransferase;
DE              EC=2.1.1.56;
DE   Includes:
DE     RecName: Full=mRNA guanylyltransferase;
DE              EC=2.7.7.-;
GN   Name=L;
OS   Australian bat lyssavirus (isolate Human/AUS/1998) (ABLV).
OC   Viruses; ssRNA negative-strand viruses; Mononegavirales;
OC   Rhabdoviridae; Lyssavirus.
OX   NCBI_TaxID=446562;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9402; Pteropus alecto (Black flying fox).
OH   NCBI_TaxID=328804; Pteropus conspicillatus (Spectacled flying fox).
OH   NCBI_TaxID=9403; Pteropus poliocephalus (Grey-headed flying fox).
OH   NCBI_TaxID=94117; Pteropus scapulatus (Little red flying fox).
OH   NCBI_TaxID=446909; Saccolaimus.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   MEDLINE=22080400; PubMed=12083841; DOI=10.1006/viro.2002.1417;
RA   Warrilow D., Smith I.L., Harrower B., Smith G.A.;
RT   "Sequence analysis of an isolate from a fatal human infection of
RT   Australian bat lyssavirus.";
RL   Virology 297:109-119(2002).
CC   -!- FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl
CC       transferase, mRNA (guanine-7-N-)-methyltransferase and poly(A)
CC       synthetase activities. The viral mRNA guanylyl transferase
CC       displays a different biochemical reaction than the cellular
CC       enzyme. The template is composed of the viral RNA tightly
CC       encapsidated by the nucleoprotein (N). Functions either as
CC       transcriptase or as replicase. The transcriptase synthesizes
CC       subsequently five subgenomic RNAs, assuring their capping and
CC       polyadenylation by a stuttering mechanism. The replicase mode is
CC       dependent on intracellular N protein concentration. In this mode,
CC       the polymerase replicates the whole viral genome without
CC       recognizing the transcriptional signals (By similarity).
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-
CC       adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
CC   -!- SUBUNIT: Interacts with the P protein to form the functional
CC       polymerase (By similarity).
CC   -!- SUBCELLULAR LOCATION: Virion. Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the rhabdoviruses protein L family.
CC   -!- SIMILARITY: Contains 1 RdRp catalytic domain.
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DR   EMBL; AF418014; AAN05310.1; -; Genomic_RNA.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0019012; C:virion; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IEA:EC.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:InterPro.
DR   GO; GO:0006370; P:mRNA capping; IEA:UniProtKB-KW.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
DR   InterPro; IPR017234; RNA-dir_RNA_pol_rhabdovir.
DR   InterPro; IPR014023; RNA_pol_cat.
DR   InterPro; IPR001016; RNA_pol_L_viral.
DR   Pfam; PF00946; Paramyx_RNA_pol; 1.
DR   PIRSF; PIRSF037546; RNA_pol_RhabdoV_sub; 1.
DR   PROSITE; PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; Methyltransferase;
KW   mRNA capping; mRNA processing; Multifunctional enzyme;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA replication;
KW   RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase;
KW   Virion.
FT   CHAIN         1   2127       Large structural protein.
FT                                /FTId=PRO_0000294414.
FT   DOMAIN      611    799       RdRp catalytic.
FT   REGION     1562   2127       Interaction with P protein (By
FT                                similarity).
SQ   SEQUENCE   2127 AA;  242676 MW;  E3777CFCDA404F4E CRC64;
     MIDPGEVYDD PIDPVEPEPE LKNNTVIPNI LRNSDYNLNS PLIEDSARLM LEWLTTGNRP
     GRLTLTDNCI RSYKTLKCYF RKVDVGSLKV GGLAAQAMIS LWLHGEHSES NRSRKCLSDL
     TQFYQKSSPI EKLLNYTLGN RGLRIPPEGV LFCLKKVDYD RAFGRYLANI YASYLFFHVI
     ILYMNALDWD EEKTILALWK DLSSVDIKKD QVKFRDPIWG SLIVTKDFVY SQNANCLFDR
     NYTLMLKDLF LSRFNSLLIL LSPPEPRYSD DLISQLCQLY IAGDNVLSMC GNSGYDVIKM
     LEPYVVNSLV QRAEGFRPLI HSLGDFPLFI RDKVGQLEGT FGPSARKFFQ VLDQFDNIHD
     LVFVYGCYRH WGHPYIDYRK GLSKLYDQVH IKKVIDGTYQ ECLASDLAKR VLRWGFDKYS
     KWYLDPKLLA QDHPLTPYIR TQTWPPKHIV DLVGNTWHRL PITQIFEIPE SMDPSEILDD
     KSHSLTRTKL ASWLAENRGG PVPSEKVIIT ALSKPPVNPR EFLKSIDLGG LPDDDLIIGL
     KPKERELKIE GRFFALMSWN LRLYFVITEK LLANYILPLF DALTMTDNLN KVFKKLIDRV
     TGQGLQDYSR VTYAFHLDYE KWNNHQRLES TKDVFSVLDQ VFGLKKVFSR THEFFQKSWV
     YYSDRSDLIG LWEDQIYCLD MSDGPTCWNG QDGGLEGLRQ KGWSLVSLLM IDRESQTRNT
     RTKILAQGDN QVLCPTYMLS SGLSQEGLLY ELESISRNAL SIYRAIEDGA SKLGLIIKKE
     ETMCSYDFLI YGKTPLFRGN ILVPESKRWA RVSCISNDQI VNLANIMSTV STNALTVAQH
     SQSLIKPMRD FLLMSVQAVF HYLLFSPILK GRVYKILSAD GDDFLLAMSR IIYLDPSLGG
     VSGMSLGRFH IRQFSDPVSE GLSFWKEIWS SSSEPWIHSL CQEAGNPDLG DRSLESFTRL
     LEDPTTLNIR GGASPTILLK DAIRKALYDE VDKVENSEFR EAILLSKTHR DNFILFLKSI
     EPLFPRFLSE LFSSSFLGIP ESIIGLIQNS RTIRRQFRRS LSRSLEESFY NSEIHGINRM
     TQVPQRIGRV WSCSSERADL LREISWGRKV VGTTVPHPSE MLTLLPKSSI SCVCGPTGSE
     NPRVSVSVLP SFDQSFFSRG PLKGYLGSST SMSTQLFHAW EKVTNVHVVK RALSLKESIN
     WFIARDSNLA QTLIKNIISL TGPQFPLEEA PVFKRTGSAL HRFKSARYSE GGYSSICPNL
     LSHISVSTDT MSDLTQDGRN FDFMFQPLML YAQTWTSELV QKDIRLKDST FHWHLRCQKC
     IRSIDDITLD TSQVFEFPDV SKRISRMVSG AVPQFQKLPE VYLKPGHFDS LCGKDKSRHI
     GSAQGLLYSI LVATHDPGYN DGTIFPVNIY SKISPKDYLR GLARGVLIGS SICFLTRMTN
     ININRPLELI SGVISYILLR LDNHPSLYVM LRESSLRSEI FSIPQKIPAA YPTTMKEGNR
     SVLCYLQHVL RYERDIITSS PENDWLWIFS DFRSTKMTYL TLITYQSHIL LQRIERSLSK
     KMRADLRQLS SLMRQVLGGH GEDSLDSGED IQRLLRDALQ RTRWVDQEVR HAAKTMTGDH
     SPSKKTSRKA GCSEWICSAQ QVAISTSSNP APVSEMDIRT LSRKLQNPLI SGLRVVQWAT
     GAHYKLKPIL DDLNAYPSFC LVVGDGSGGI SRTVLNMFPD AKLVFNSLLE VSDLMASGTH
     PLPPSAIMSG GDDIVSRVVD FESIWEKPSD LRNLSTWRYF QSVQSKLNMS YDLIICDAEV
     TDIISVNKIT LLMSDFVLSI DGPLDLIFKS YGTMLVNPDY KAIQHLSRAF PKATGYITQL
     TSSFSSELYL RFSKRGKFFR DVEYLTSSTL REMSLVLFNC SSPKSELQRA RSLNYQDLIR
     GFPEEIVSNP YNEMIITLID SEVESFLVHK MVDDLELQRG TLSKMSIIIA IVIVYSNRVF
     NVSKPLSDPV FYPPSDPKIL RHFNICCSTM LYLSTILGDV PNFARLHELY NSPITYYFKK
     QIIRGSIYLS WSWSDDTSVF KRVSCNSNLS LSSHWIRLIY KIIKTTRFTG SHVDLSKEVE
     KHLKGYNRWI TFNDVRSRSS LLDYSCL
//