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[1]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND COFACTOR.
DOI=10.1074/jbc.M302149200; PubMed=12660232 [NCBI, ExPASy, EBI, Israel, Japan]
Wu T.,
Yankovskaya V.,
McIntire W.S.;
"Cloning, sequencing, and heterologous expression of the murine peroxisomal flavoprotein, N(1)-acetylated polyamine oxidase.";
J. Biol. Chem. 278:20514-20525(2003).
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[2]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
STRAIN=C57BL/6J;
TISSUE=Head;
DOI=10.1126/science.1112014; PubMed=16141072 [NCBI, ExPASy, EBI, Israel, Japan]
Carninci P.,
Kasukawa T.,
Katayama S.,
Gough J.,
Frith M.C.,
Maeda N.,
Oyama R.,
Ravasi T.,
Lenhard B.,
Wells C.,
Kodzius R.,
Shimokawa K.,
Bajic V.B.,
Brenner S.E.,
Batalov S.,
Forrest A.R.,
Zavolan M.,
Davis M.J.,
Wilming L.G., ![]()
Aidinis V.,
Allen J.E.,
Ambesi-Impiombato A.,
Apweiler R.,
Aturaliya R.N.,
Bailey T.L.,
Bansal M.,
Baxter L.,
Beisel K.W.,
Bersano T.,
Bono H.,
Chalk A.M.,
Chiu K.P.,
Choudhary V.,
Christoffels A.,
Clutterbuck D.R.,
Crowe M.L.,
Dalla E.,
Dalrymple B.P.,
de Bono B.,
Della Gatta G.,
di Bernardo D.,
Down T.,
Engstrom P.,
Fagiolini M.,
Faulkner G.,
Fletcher C.F.,
Fukushima T.,
Furuno M.,
Futaki S.,
Gariboldi M.,
Georgii-Hemming P.,
Gingeras T.R.,
Gojobori T.,
Green R.E.,
Gustincich S.,
Harbers M.,
Hayashi Y.,
Hensch T.K.,
Hirokawa N.,
Hill D.,
Huminiecki L.,
Iacono M.,
Ikeo K.,
Iwama A.,
Ishikawa T.,
Jakt M.,
Kanapin A.,
Katoh M.,
Kawasawa Y.,
Kelso J.,
Kitamura H.,
Kitano H.,
Kollias G.,
Krishnan S.P.,
Kruger A.,
Kummerfeld S.K.,
Kurochkin I.V.,
Lareau L.F.,
Lazarevic D.,
Lipovich L.,
Liu J.,
Liuni S.,
McWilliam S.,
Madan Babu M.,
Madera M.,
Marchionni L.,
Matsuda H.,
Matsuzawa S.,
Miki H.,
Mignone F.,
Miyake S.,
Morris K.,
Mottagui-Tabar S.,
Mulder N.,
Nakano N.,
Nakauchi H.,
Ng P.,
Nilsson R.,
Nishiguchi S.,
Nishikawa S.,
Nori F.,
Ohara O.,
Okazaki Y.,
Orlando V.,
Pang K.C.,
Pavan W.J.,
Pavesi G.,
Pesole G.,
Petrovsky N.,
Piazza S.,
Reed J.,
Reid J.F.,
Ring B.Z.,
Ringwald M.,
Rost B.,
Ruan Y.,
Salzberg S.L.,
Sandelin A.,
Schneider C.,
Schoenbach C.,
Sekiguchi K.,
Semple C.A.,
Seno S.,
Sessa L.,
Sheng Y.,
Shibata Y.,
Shimada H.,
Shimada K.,
Silva D.,
Sinclair B.,
Sperling S.,
Stupka E.,
Sugiura K.,
Sultana R.,
Takenaka Y.,
Taki K.,
Tammoja K.,
Tan S.L.,
Tang S.,
Taylor M.S.,
Tegner J.,
Teichmann S.A.,
Ueda H.R.,
van Nimwegen E.,
Verardo R.,
Wei C.L.,
Yagi K.,
Yamanishi H.,
Zabarovsky E.,
Zhu S.,
Zimmer A.,
Hide W.,
Bult C.,
Grimmond S.M.,
Teasdale R.D.,
Liu E.T.,
Brusic V.,
Quackenbush J.,
Wahlestedt C.,
Mattick J.S.,
Hume D.A.,
Kai C.,
Sasaki D.,
Tomaru Y.,
Fukuda S.,
Kanamori-Katayama M.,
Suzuki M.,
Aoki J.,
Arakawa T.,
Iida J.,
Imamura K.,
Itoh M.,
Kato T.,
Kawaji H.,
Kawagashira N.,
Kawashima T.,
Kojima M.,
Kondo S.,
Konno H.,
Nakano K.,
Ninomiya N.,
Nishio T.,
Okada M.,
Plessy C.,
Shibata K.,
Shiraki T.,
Suzuki S.,
Tagami M.,
Waki K.,
Watahiki A.,
Okamura-Oho Y.,
Suzuki H.,
Kawai J.,
Hayashizaki Y.;
"The transcriptional landscape of the mammalian genome.";
Science 309:1559-1563(2005).
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[3]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
STRAIN=FVB/N;
TISSUE=Colon, and Mammary gland;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
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- FUNCTION: Flavoenzyme which catalyzes the oxidation of N(1)-acetylspermine to spermidine and is thus involved in the polyamine back-conversion. Can also oxidize N(1)-acetylspermidine to putrescine. Substrate specificity: N(1)-acetylspermine = N(1)-acetylspermidine > N(1),N(12)-diacylspermine >> spermine. Does not oxidize spermidine. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs.
- CATALYTIC ACTIVITY: N1-acetylspermine + O2 + H2O = N1-acetylspermidine + 3-aminopropanal + H2O2.
- COFACTOR: FAD.
- PATHWAY: Amine and polyamine metabolism; spermine metabolism.
- SUBUNIT: Monomer.
- SUBCELLULAR LOCATION: Peroxisome (By similarity). Cytoplasm (By similarity).
- ALTERNATIVE PRODUCTS:
2 named isoforms [FASTA] produced by alternative splicing.
- TISSUE SPECIFICITY: Widely expressed at different developmental stages. Expressed at high level in the liver and the stomach, expressed at lower level in heart, spleen, thymus, small intestine, muscle, pancreas, uterus, and breast and expressed at very low level in brain, kidney, lung, testis, skin, adrenal gland and prostate gland.
- DEVELOPMENTAL STAGE: Expression increased during embryonic development: there is a gradual increase in the tissues on going from 8.5 to 19 day embryos. In the breast, expression is very low in virgin mouse and quite high in pregnant mouse, but decreases in lactating and involuting breasts.
- INDUCTION: By polyamine analogs.
- MISCELLANEOUS: Oxidizes N(1)-acetylated polyamines on the exo-side of their N(4)-amino groups. Plant PAO oxidizes spermine on the endo-side of the N(4)-nitrogen (By similarity).
- MISCELLANEOUS: N-ethylated polyamines are also good substrates for this enzyme: they have been used for cancer clinical trials. They down-regulate polyamine biosynthetic enzymes, but dramatically up-regulate SSAT synthesis, which results in mammalian cells becoming apaptotic.
- SIMILARITY: Belongs to the flavin monoamine oxidase family.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 504 AA [This is the length of the unprocessed precursor] |
Molecular weight: 55447 Da [This is the MW of the unprocessed precursor] |
CRC64: B40BD34C7A0B98F1 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MAFPGPRVLV VGSGIAGLGA AQKLCSHRAA PHLRVLEATA SAGGRIRSER CFGGVVELGA
70 80 90 100 110 120
HWIHGPSQDN PVFQLAAEFG LLGEKELSEE NQLVDTGGHV ALPSMIWSSS GTSVSLELMT
130 140 150 160 170 180
EMARLFYGLI ERTREFLNES ETPMASVGEF LKKEISQQVA SWTEDDEDTR KRKLAILNTF
190 200 210 220 230 240
FNIECCVSGT HSMDLVALAP FGEYTVLPGL DCILAGGYQG LTDRILASLP KDTVAFDKPV
250 260 270 280 290 300
KTIHWNGSFQ EAAFPGETFP VLVECEDGAR LPAHHVIVTV PLGFLKEHQD TFFEPPLPAK
310 320 330 340 350 360
KAEAIKKLGF GTNNKIFLEF EEPFWEPDCQ FIQVVWEDTS PLQDTALSLQ DTWFKKLIGF
370 380 390 400 410 420
LVQPSFESSH VLCGFIAGLE SEFMETLSDE EVLLSLTQVL RRVTGNPQLP AAKSVRRSQW
430 440 450 460 470 480
HSAPYTRGSY SYVAVGSTGD DLDLMAQPLP EDGTGTQLQV LFAGEATHRT FYSTTHGALL
490 500
SGWREADRLV SLWDSQVEQS RPRL
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Q8C0L6 in FASTA format |
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