NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
STRAIN=C57BL/6;
TISSUE=Brain;
DOI=10.1016/j.gene.2003.08.005; PubMed=14637003 [NCBI, ExPASy, EBI, Israel, Japan]
Comelli E.M., Amado M., Lustig S.R., Paulson J.C.;
"Identification and expression of Neu4, a novel murine sialidase.";
Gene 321:155-161(2003).

[2]

NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
STRAIN=C57BL/6J;
TISSUE=Diencephalon;
DOI=10.1126/science.1112014; PubMed=16141072 [NCBI, ExPASy, EBI, Israel, Japan]
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
"The transcriptional landscape of the mammalian genome.";
Science 309:1559-1563(2005).

Comments

FUNCTION: Has sialidase activity towards synthetic substrates, such as 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid (4-MU-NANA or 4MU-NeuAc) (By similarity).
CATALYTIC ACTIVITY: Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein (By similarity).

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name	1
Isoform ID	Q8BZL1-1
This is the isoform sequence displayed in this entry.

Name	2
Isoform ID	Q8BZL1-2
Note: No experimental confirmation available.
Features which should be applied to build the isoform sequence: VSP_010495.

TISSUE SPECIFICITY: Expressed predominantly in brain.
SIMILARITY: Belongs to the glycosyl hydrolase 33 family [view classification].
SIMILARITY: Contains 3 BNR repeats.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AY258421; AAP81169.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AK034236; BAC28641.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

UniGene

Mm.214565

3D structure databases

ModBase

Q8BZL1.

Organism-specific databases

MGI

MGI:2661364; Neu4.

Gene expression databases

ArrayExpress

Q8BZL1; -.

CleanEx

MM_NEU4; -.

Ontologies

GO:0005764;	Cellular component: lysosome (inferred from sequence or structural similarity from UniProtKB).
GO:0019866;	Cellular component: organelle inner membrane (inferred from sequence or structural similarity from UniProtKB).
GO:0004308;	Molecular function: exo-alpha-sialidase activity (inferred from direct assay from MGI).
GO:0008152;	Biological process: metabolic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

Genome annotation databases

Ensembl

ENSMUSG00000034000; Mus musculus. [Contig view]

Phylogenomic databases

HOGENOM

Q8BZL1; -.

HOVERGEN

Q8BZL1; -.

Other

SOURCE

Neu4; Mus musculus.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Glycosidase; Hydrolase; Membrane; Repeat.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 501`	`501`	`Sialidase-4.`	`PRO_0000208907`
`REPEAT`	`150 161`	`12`	`BNR 1.`
`REPEAT`	`223 234`	`12`	`BNR 2.`
`REPEAT`	`270 281`	`12`	`BNR 3.`
`MOTIF`	`45 48`	`4`	`FRIP motif.`
`ACT_SITE`	`71 71`		`Proton acceptor (By similarity).`
`ACT_SITE`	`436 436`		`Nucleophile (By similarity).`
`ACT_SITE`	`457 457`		`Potential.`
`BINDING`	`46 46`		`Substrate (By similarity).`
`BINDING`	`406 406`		`Substrate (By similarity).`
`VAR_SEQ`	`1 23`		`Missing (in isoform 2).`	`VSP_010495`

Sequence information

Length: 501 AA [This is the length of the unprocessed precursor]

Molecular weight: 54996 Da [This is the MW of the unprocessed precursor]

CRC64: 9CF4B78F19DF3BC2 [This is a checksum on the sequence]

        10         20         30         40         50         60 
METAGAPFCF HVDSLVPCSY WKVMGPTRVP RRTVLFQRER TGLTYRVPAL LCVPPRPTLL 

        70         80         90        100        110        120 
AFAEQRLSPD DSHAHRLVLR RGTLTRGSVR WGTLSVLETA VLEEHRSMNP CPVLDEHSGT 

       130        140        150        160        170        180 
IFLFFIAVLG HTPEAVQIAT GKNAARLCCV TSCDAGLTWG SVRDLTEEAI GAALQDWATF 

       190        200        210        220        230        240 
AVGPGHGVQL RSGRLLVPAY TYHVDRRECF GKICWTSPHS LAFYSDDHGI SWHCGGLVPN 

       250        260        270        280        290        300 
LRSGECQLAA VDGDFLYCNA RSPLGNRVQA LSADEGTSFL PGELVPTLAE TARGCQGSIV 

       310        320        330        340        350        360 
GFLAPPSIEP QDDRWTGSPR NTPHSPCFNL RVQESSGEGA RGLLERWMPR LPLCYPQSRS 

       370        380        390        400        410        420 
PENHGLEPGS DGDKTSWTPE CPMSSDSMLQ SPTWLLYSHP AGRRARLHMG IYLSRSPLDP 

       430        440        450        460        470        480 
HSWTEPWVIY EGPSGYSDLA FLGPMPGASL VFACLFESGT RTSYEDISFC LFSLADVLEN 

       490        500 
VPTGLEMLSL RDKAQGHCWP S

Q8BZL1 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools