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UniProtKB/Swiss-Prot entry Q8BMF3

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MAON_MOUSE
Primary accession number Q8BMF3
Secondary accession numbers None
Integrated into Swiss-Prot on May 10, 2005
Sequence was last modified on March 1, 2003 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 45)
Name and origin of the protein
Protein name NADP-dependent malic enzyme, mitochondrial [Precursor]
Synonyms NADP-ME
EC 1.1.1.40
Malic enzyme 3
Gene name
Name: Me3
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=C57BL/6J;
TISSUE=Olfactory bulb;
DOI=10.1126/science.1112014; PubMed=16141072 [NCBI, ExPASy, EBI, Israel, Japan]
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
"The transcriptional landscape of the mammalian genome.";
Science 309:1559-1563(2005).
[2]
 PROTEIN SEQUENCE OF 71-92 AND 481-506, AND MASS SPECTROMETRY.
TISSUE=Hippocampus;
Lubec G., Klug S.;
Submitted (MAR-2007) to UniProtKB.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AK032196; BAC27751.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_852072.1; -.
UniGene Mm.334011
3D structure databases
HSSP P40927; 1GQ2. [HSSP ENTRY / PDB]
ModBase Q8BMF3.
Organism-specific databases
MGI MGI:1916679; Me3.
Gene expression databases
ArrayExpress Q8BMF3; -.
CleanEx MM_ME3; -.
GermOnline ENSMUSG00000030621; Mus musculus.
Ontologies
GO
GO:0004473; Molecular function: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity (inferred from sequence or structural similarity from UniProtKB).
GO:0006108; Biological process: malate metabolic process (inferred from sequence or structural similarity from UniProtKB).
GO:0055114; Biological process: oxidation reduction (inferred from sequence or structural similarity from UniProtKB).
GO:0006090; Biological process: pyruvate metabolic process (inferred from sequence or structural similarity from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR001891; Malic_OxRdtase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF00390; malic; 1.
PF03949; Malic_M; 1.
Pfam graphical view of domain structure.
PRINTS PR00072; MALOXRDTASE.
PROSITE PS00331; MALIC_ENZYMES; 1.
BLOCKS Q8BMF3.
Genome annotation databases
Ensembl ENSMUSG00000030621; Mus musculus. [Contig view]
GeneID 109264; -.
KEGG mmu:109264; -.
Phylogenomic databases
HOGENOM Q8BMF3; -.
HOVERGEN Q8BMF3; -.
Other
SOURCE Me3; Mus musculus.
ProtoNet Q8BMF3.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Direct protein sequencing; Metal-binding; Mitochondrion; NADP; Oxidoreductase; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1     ?        Mitochondrion (Potential). 
CHAIN   ?   604        NADP-dependent malic enzyme, mitochondrial. PRO_0000018540
ACT_SITE   137   137        Proton donor (By similarity). 
ACT_SITE   208   208        Proton acceptor (By similarity). 
METAL   280   280        Divalent metal cation (By similarity). 
METAL   281   281        Divalent metal cation (By similarity). 
METAL   304   304        Divalent metal cation (By similarity). 
BINDING   190   190        NADP (By similarity). 
BINDING   304   304        NADP (By similarity). 
BINDING   443   443        NADP (By similarity). 
SITE   304   304  1     Important for activity (By similarity). 
Sequence information
Length: 604 AA [This is the length of the unprocessed precursor] Molecular weight: 67180 Da [This is the MW of the unprocessed precursor] CRC64: 14A0AC946D53EB21 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MGAALGTGAR LTSVPRIACS SLRRQAPSAP AQGCHSKSGP PRPVPLKKRG YDVTRNPHLN 

        70         80         90        100        110        120 
KGMAFTLEER LQLGIHGLIP PCFLSQDVQL LRIMRYYENQ QSDLDKYIIL MTHQDRNEKL 

       130        140        150        160        170        180 
FYRVLTSDVE KFMPIVYTPT VGLACQHYGL TFRRPRGLFI TIHDKGHIAT MLNSWPEDNI 

       190        200        210        220        230        240 
KAVVVTDGER ILGLGDLGCY GMGIPVGKLA LYTACGGVNP QQCLPVLLDV GTNNEELLRD 

       250        260        270        280        290        300 
PLYIGLKHQR VRGEEYDDLL DEFMQAVTDK FGINCLIQFE DFANANAFRL LNKYRNKYCM 

       310        320        330        340        350        360 
FNDDIQGTAS VAVAGILAAL RITKNRLSNH VFVFQGADEA AMGIAHLLVM ALEKEGIPKT 

       370        380        390        400        410        420 
EAIKKIWMVD SKGLIVKGRS HLNHEKEMFA QDHPEVNSLE EVVRLVKPTA IIGVAAIAGA 

       430        440        450        460        470        480 
FTEQILRDMA SFHERPIVFA LSNPTSKAEC TAEKCYRVTE GRGIFASGSP FKSVTLEDGR 

       490        500        510        520        530        540 
TFTPGQGNNA YVFPGVALGV IAGGIRHIPD EIFLLTAEQI AQEVSEQHLS QGRLYPPLST 

       550        560        570        580        590        600 
IRDVSLRIAV KVLDYAYKHN LASYYPEPKD KEAFVKSLIY TPDYDSFSLD TYSWPKEAMS 


VQKV
Q8BMF3 in FASTA format

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