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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS REP; REP'; REP3A; REP3B AND REP3C), AND ALTERNATIVE PROMOTER USAGE.
STRAIN=Isolate PCV/688;
DOI=10.1006/viro.2002.1733; PubMed=12504550 [NCBI, ExPASy, EBI, Israel, Japan]
Cheung A.K.;
"Transcriptional analysis of porcine circovirus type 2.";
Virology 305:168-180(2003).
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[2]
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FUNCTION, AND MUTAGENESIS OF 179-GLY-LYS-180.
STRAIN=Isolate PCV/688;
DOI=10.1016/S0042-6822(03)00373-8; PubMed=12954212 [NCBI, ExPASy, EBI, Israel, Japan]
Cheung A.K.;
"The essential and nonessential transcription units for viral protein synthesis and DNA replication of porcine circovirus type 2.";
Virology 313:452-459(2003).
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[3]
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STRUCTURE BY NMR OF 1-116, COFACTOR, AND CHARACTERIZATION.
DOI=10.1016/j.jmb.2007.01.002; PubMed=17275023 [NCBI, ExPASy, EBI, Israel, Japan]
Vega-Rocha S.,
Byeon I.-J.L.,
Gronenborn B.,
Gronenborn A.M.,
Campos-Olivas R.;
"Solution structure, divalent metal and DNA binding of the endonuclease domain from the replication initiation protein from porcine circovirus 2.";
J. Mol. Biol. 367:473-487(2007).
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- FUNCTION: Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep and/or Rep' binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-AGTATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. ATPase activity is probably carried by the isoform Rep (By similarity).
- CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.
- COFACTOR: Divalent metal cations, possibly magnesium or manganese (Probable).
- SUBCELLULAR LOCATION: Nucleus (Potential).
- ALTERNATIVE PRODUCTS:
8 named isoforms [FASTA] produced by alternative promoter usage and alternative splicing.
| Name | Rep |
| Isoform ID | Q8BB16-1 |
| Note: Produced by alternative promoter usage. |
| This is the isoform sequence displayed in this entry. |
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| Name | Rep' |
| Isoform ID | Q8BB16-2 |
| Note: Produced by alternative splicing of isoform Rep. |
| Features which should be applied to build the isoform sequence: VSP_015884, VSP_015885. |
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| Name | NS0 |
| Isoform ID | Q8BB12-1 |
| Note: Produced by alternative promoter usage. |
| This isoform is stored in UniProtKB/Swiss-Prot entry Q8BB12. |
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| Name | NS642 |
| Isoform ID | Q8BB16-6 |
| Note: Produced by alternative promoter usage. |
| The sequence of this isoform is not described. |
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| Name | NS462 |
| Isoform ID | Q8BB16-7 |
| Note: Produced by alternative promoter usage. |
| The sequence of this isoform is not described. |
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| Name | Rep3a |
| Isoform ID | Q8BB16-3 |
| Note: Produced by alternative splicing of isoform Rep. |
| Features which should be applied to build the isoform sequence: VSP_015883. |
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| Name | Rep3b |
| Isoform ID | Q8BB16-4 |
| Note: Produced by alternative splicing of isoform Rep. |
| Features which should be applied to build the isoform sequence: VSP_015882. |
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| Name | Rep3c |
| Isoform ID | Q8BB16-5 |
| Note: Produced by alternative splicing of isoform Rep. |
| The sequence of this isoform is not described. |
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- DOMAIN: There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.
- SIMILARITY: Belongs to the nanoviruses/circoviruses replication-associated protein family.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 314 AA [This is the length of the unprocessed precursor] |
Molecular weight: 35762 Da [This is the MW of the unprocessed precursor] |
CRC64: 3FE9D85F2CD656FC [This is a checksum on the sequence] |
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10 20 30 40 50 60
MPSKKNGRSG PQPHKRWVFT LNNPSEDERK KIREPPISLF DYFIVGEEGN EEGRTPHLQG
70 80 90 100 110 120
FANFVKKQTF NKVKWYLGAR CHIEKAKGTD QQNKEYCSKE GNLLIECGAP RSQGQRSDLS
130 140 150 160 170 180
TAVSTLLESG SLVTVAEQHP VTFVRNFRGL AELLKVSGKM QKRDWKTNVH VIVGPPGCGK
190 200 210 220 230 240
SKWAANFADP ETTYWKPPRN KWWDGYHGEE VVVIDDFYGW LPWDDLLRLC DRYPLTVETK
250 260 270 280 290 300
GGTVPFLARS ILITSNQTPL EWYSSTAVPA VEALYRRITS LVFWKNATEQ STEEGGQFVT
310
LSPPCPEFPY EINY
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Q8BB16 in FASTA format |
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