ID   L_VSIVC                 Reviewed;        2109 AA.
AC   Q8B0H0;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-NOV-2008, entry version 29.
DE   RecName: Full=Large structural protein;
DE            Short=Protein L;
DE   AltName: Full=Transcriptase;
DE   AltName: Full=Replicase;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=mRNA (guanine-N(7)-)-methyltransferase;
DE              EC=2.1.1.56;
DE   Includes:
DE     RecName: Full=mRNA guanylyltransferase;
DE              EC=2.7.7.-;
GN   Name=L;
OS   Vesicular stomatitis Indiana virus (strain 94GUB Central America)
OS   (VSIV).
OC   Viruses; ssRNA negative-strand viruses; Mononegavirales;
OC   Rhabdoviridae; Dimarhabdovirus supergroup; Vesiculovirus.
OX   NCBI_TaxID=434489;
OH   NCBI_TaxID=7158; Aedes.
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
OH   NCBI_TaxID=58271; Culicoides.
OH   NCBI_TaxID=9793; Equus asinus (Donkey).
OH   NCBI_TaxID=9796; Equus caballus (Horse).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=252607; Lutzomyia.
OH   NCBI_TaxID=7370; Musca domestica (House fly).
OH   NCBI_TaxID=7190; Simuliidae (black flies).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   MEDLINE=22222605; PubMed=12237430;
RA   Rodriguez L.L., Pauszek S.J., Bunch T.A., Schumann K.R.;
RT   "Full-length genome analysis of natural isolates of vesicular
RT   stomatitis virus (Indiana 1 serotype) from North, Central and South
RT   America.";
RL   J. Gen. Virol. 83:2475-2483(2002).
CC   -!- FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl
CC       transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A)
CC       synthetase activities. The viral mRNA guanylyl transferase
CC       displays a different biochemical reaction than the cellular
CC       enzyme. The template is composed of the viral RNA tightly
CC       encapsidated by the nucleoprotein (N). Functions either as
CC       transcriptase or as replicase. The transcriptase synthesizes
CC       subsequently five subgenomic RNAs, assuring their capping and
CC       polyadenylation by a stuttering mechanism. The replicase mode is
CC       dependent on intracellular N protein concentration. In this mode,
CC       the polymerase replicates the whole viral genome without
CC       recognizing the transcriptional signals (By similarity).
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-
CC       adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
CC   -!- SUBUNIT: Interacts with the P protein to form the functional
CC       polymerase (By similarity).
CC   -!- SUBCELLULAR LOCATION: Virion. Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the rhabdoviridae protein L family.
CC   -!- SIMILARITY: Contains 1 RdRp catalytic domain.
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DR   EMBL; AF473866; AAN16994.1; -; Genomic_RNA.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0019012; C:virion; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IEA:EC.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:InterPro.
DR   GO; GO:0006370; P:mRNA capping; IEA:UniProtKB-KW.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
DR   InterPro; IPR017234; RNA-dir_RNA_pol_rhabdovir.
DR   InterPro; IPR014023; RNA_pol_cat.
DR   InterPro; IPR001016; RNA_pol_L_viral.
DR   Pfam; PF00946; Paramyx_RNA_pol; 1.
DR   PIRSF; PIRSF037546; RNA_pol_RhabdoV_sub; 1.
DR   PROSITE; PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; Methyltransferase; mRNA capping;
KW   mRNA processing; Multifunctional enzyme; Nucleotide-binding;
KW   Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase;
KW   S-adenosyl-L-methionine; Transferase; Virion.
FT   CHAIN         1   2109       Large structural protein.
FT                                /FTId=PRO_0000287263.
FT   DOMAIN      598    784       RdRp catalytic.
SQ   SEQUENCE   2109 AA;  240943 MW;  F3AF49C781DFC5C8 CRC64;
     MEIHDFEADE FIDFNEDDYA TREFLNPDER MTYLNHADYN LNSPLISDDI DNLIRKFNSL
     PIPSMWDSKN WDGVLEMLTS CQANPIPTSQ MHKWMGSWIM SDNHDASQGY SFLHEVDKEA
     EITFDVVETF IRGWGNKLIE YTKKEKWTDS FKILAYLCQK FLDLHKLTLI LNAVSEVELL
     NLARTFKGKV RVSSHGTNIC RLRVPSLGPT FISEGWAYFK KLDIIMDRNF LLMVKDVIIG
     RMQTVLSMVC RTDNLFSEQD IFSLLNIYRI GDKIVERQGN FSYDLIKMVE PICNLKLMKL
     ARESRPLVPQ FPHFENHIQT SVDEGAKIDR GIKFLHDQIM SVKTVDLTLV IYGSFRHWGH
     PFIDYYAGLE KLHSQVTMKK DIDVSYAKAL ASDLARIVLF QQFNDHKKWF VNGDLLPHDH
     PFKSHVKENT WPTAAQIQDF GDKWHELPLI KCFEIPDLLD PSIIYSDKSH SMNRSEVLKH
     VRMNPNTPIP SKKVLQTMLD TKATNWKEFL KEIDEKGLDD DDLIIGLKGK ERELKLAGRF
     FSLMSWKLRE YFVITEYLIK THFVPMFKGL TMADDLTAVI KKMLDSSSGQ GLKSYEAICI
     ANHIDYEKWN NHQRKLSNGP VFRVMGQFLG YPSLIERTHE FFEKSLIYYN GRPDLMRVHN
     NTLVNSTSQR VCWQGQEGGL EGLRQKGWSI LNLLVIQREA KIRNTAVKVL AQGDNQVICT
     QYKTKKSRNV VELQSALNQM VSNNEKIMTA IKIGTGKLGL LINDDETMQS ADYLNYGKIP
     IFRGVIRGLE TKRWSRVTCV TNDQIPTCAN IMSSVSTNAL TVAHFAENPI NAMIQYNYFG
     TFARLLLMMH DPALRQSLYE VQDKIPGLHS STFKYAMLYL DPSIGGVSGM SLSRFLIRAF
     PDPVTESLSF WRFIHIHARS EHLKEMSAIF GNPEIAKFRI THIDKLVEDP TSLNIAMGMS
     PANLLKTEVK KCLLESRQTI RNQVIKDATI YLYHEEDRLR SFLWSINPLF PRFLSEFKSG
     TFLGVADGLI SLFQNSRTIR NSFKKKYHRE LDDLIVRSEV SSLIHLGKLH LRRGSYKMWT
     CSATHADTLR YKSWGRTVIG TTVPHPLEML GPQHRKETPC APCNTSGFNY VSVHCPDGIH
     DVFSSRGPLP AYLGSKTSES TSILQPWERE SKVPLIKRAT RLRDAISWFV EPDSKLAMTI
     LSNIRSLTGE EWTKRQRGFK RTGSALHRFS TSRMSHGGFA SQSTAALTRL MATTDTMRDL
     GDQNFDFLFQ ATLLYAQITT TVARDGWTTS CTDHYHITCK SCLRPIEEIT LDSSMDYTPP
     DVSHVLKTWR NGEGSWGQEI KQIYPLEGNW KNLAPAEQSY QVGRCIGFLY GDLAYRKSTH
     AEDSSLFPLS IQNRVRGRGF LKGLLDGLMR ASCCQVIHRR SLAHLKRPAN AVYGGLIYLI
     DKLSVSPPFL SLTRSGPIRD ELETIPHKIP TSYPTSNRDM GVIVRNYFKY QCRLIEKGKY
     RSHYSQLWLF SDVLSIDFLG PFSISTTLLK ILYKSSLSGK DKNELRELAN LSSLLRSGEG
     WEDIHVKFFT KDILLCPEEI RHACKFGIAK DNNKDMSYPP WGRESRGTIT TIPVYYTATH
     YPKMLEMPPR IQNPLLSGIR LGQLPTGAHY KIRSILHGMG IHYRDFLSCG DGSGGITAAL
     LRENVHSRGI FNSLLELSGS VMRGASPEPP SALETLGGDK SRCVNGETCW EHPSDLCDPR
     TWDYFLRLKA GLGLQIDLIV MDMEVRDYSA SLKIETNVRN YVHRILDEQG VLIYKTYGTY
     ICESEKNAVT ILGPMFRTVD LVQTEFSSSQ TSEVYMVCKS LKKLIDEPHP DWSSVNESWK
     NLYAFQSSEQ EFARARKVST YFTLTGIPSQ FIPDPFVNLE TMLQIFGVPT GVSHAAALKS
     SDRPADLLTI GLFYMAIISY YNINHIRVGP VPPNPPSDGT AQNVGIAITG ISLWLSLMEK
     DIPLYQQCLA VIQQSFPIRW EAVSVKGGYK QKWSTRGDGL PKDTRISDSL APIGNWIRSL
     ELVRNQVRLN PFNEILFNQL CRTVDNHLRW SNLRKNTGMI EWVNRRISTE DRSILILKSD
     LHEENSWRD
//