RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Vesicular stomatitis Indiana virus (strain 94GUB Central America) (VSIV)

[TaxID: 434489]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; Dimarhabdovirus supergroup; Vesiculovirus.

Virus hosts

Aedes [TaxID: 7158]
Bos taurus (Bovine) [TaxID: 9913]
Culicoides [TaxID: 58271]
Equus asinus (Donkey) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]
Homo sapiens (Human) [TaxID: 9606]
Lutzomyia [TaxID: 252607]
Musca domestica (House fly) [TaxID: 7370]
Simuliidae (black flies) [TaxID: 7190]
Sus scrofa (Pig) [TaxID: 9823]

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC RNA].
PubMed=12237430 [NCBI, ExPASy, EBI, Israel, Japan]
Rodriguez L.L., Pauszek S.J., Bunch T.A., Schumann K.R.;
"Full-length genome analysis of natural isolates of vesicular stomatitis virus (Indiana 1 serotype) from North, Central and South America.";
J. Gen. Virol. 83:2475-2483(2002).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein to form the functional polymerase (By similarity).
SUBCELLULAR LOCATION: Virion. Cytoplasm (By similarity).
SIMILARITY: Belongs to the rhabdoviridae protein L family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF473866; AAN16994.1; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q8B0H0.

Family and domain databases

InterPro

IPR017234; RNA-dir_RNA_pol_rhabdovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF037546; RNA_pol_RhabdoV_sub; 1.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2109`	`2109`	`Large structural protein.`	`PRO_0000287263`
`DOMAIN`	`598 784`	`187`	`RdRp catalytic.`

Sequence information

Length: 2109 AA [This is the length of the unprocessed precursor]

Molecular weight: 240943 Da [This is the MW of the unprocessed precursor]

CRC64: F3AF49C781DFC5C8 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MEIHDFEADE FIDFNEDDYA TREFLNPDER MTYLNHADYN LNSPLISDDI DNLIRKFNSL 

        70         80         90        100        110        120 
PIPSMWDSKN WDGVLEMLTS CQANPIPTSQ MHKWMGSWIM SDNHDASQGY SFLHEVDKEA 

       130        140        150        160        170        180 
EITFDVVETF IRGWGNKLIE YTKKEKWTDS FKILAYLCQK FLDLHKLTLI LNAVSEVELL 

       190        200        210        220        230        240 
NLARTFKGKV RVSSHGTNIC RLRVPSLGPT FISEGWAYFK KLDIIMDRNF LLMVKDVIIG 

       250        260        270        280        290        300 
RMQTVLSMVC RTDNLFSEQD IFSLLNIYRI GDKIVERQGN FSYDLIKMVE PICNLKLMKL 

       310        320        330        340        350        360 
ARESRPLVPQ FPHFENHIQT SVDEGAKIDR GIKFLHDQIM SVKTVDLTLV IYGSFRHWGH 

       370        380        390        400        410        420 
PFIDYYAGLE KLHSQVTMKK DIDVSYAKAL ASDLARIVLF QQFNDHKKWF VNGDLLPHDH 

       430        440        450        460        470        480 
PFKSHVKENT WPTAAQIQDF GDKWHELPLI KCFEIPDLLD PSIIYSDKSH SMNRSEVLKH 

       490        500        510        520        530        540 
VRMNPNTPIP SKKVLQTMLD TKATNWKEFL KEIDEKGLDD DDLIIGLKGK ERELKLAGRF 

       550        560        570        580        590        600 
FSLMSWKLRE YFVITEYLIK THFVPMFKGL TMADDLTAVI KKMLDSSSGQ GLKSYEAICI 

       610        620        630        640        650        660 
ANHIDYEKWN NHQRKLSNGP VFRVMGQFLG YPSLIERTHE FFEKSLIYYN GRPDLMRVHN 

       670        680        690        700        710        720 
NTLVNSTSQR VCWQGQEGGL EGLRQKGWSI LNLLVIQREA KIRNTAVKVL AQGDNQVICT 

       730        740        750        760        770        780 
QYKTKKSRNV VELQSALNQM VSNNEKIMTA IKIGTGKLGL LINDDETMQS ADYLNYGKIP 

       790        800        810        820        830        840 
IFRGVIRGLE TKRWSRVTCV TNDQIPTCAN IMSSVSTNAL TVAHFAENPI NAMIQYNYFG 

       850        860        870        880        890        900 
TFARLLLMMH DPALRQSLYE VQDKIPGLHS STFKYAMLYL DPSIGGVSGM SLSRFLIRAF 

       910        920        930        940        950        960 
PDPVTESLSF WRFIHIHARS EHLKEMSAIF GNPEIAKFRI THIDKLVEDP TSLNIAMGMS 

       970        980        990       1000       1010       1020 
PANLLKTEVK KCLLESRQTI RNQVIKDATI YLYHEEDRLR SFLWSINPLF PRFLSEFKSG 

      1030       1040       1050       1060       1070       1080 
TFLGVADGLI SLFQNSRTIR NSFKKKYHRE LDDLIVRSEV SSLIHLGKLH LRRGSYKMWT 

      1090       1100       1110       1120       1130       1140 
CSATHADTLR YKSWGRTVIG TTVPHPLEML GPQHRKETPC APCNTSGFNY VSVHCPDGIH 

      1150       1160       1170       1180       1190       1200 
DVFSSRGPLP AYLGSKTSES TSILQPWERE SKVPLIKRAT RLRDAISWFV EPDSKLAMTI 

      1210       1220       1230       1240       1250       1260 
LSNIRSLTGE EWTKRQRGFK RTGSALHRFS TSRMSHGGFA SQSTAALTRL MATTDTMRDL 

      1270       1280       1290       1300       1310       1320 
GDQNFDFLFQ ATLLYAQITT TVARDGWTTS CTDHYHITCK SCLRPIEEIT LDSSMDYTPP 

      1330       1340       1350       1360       1370       1380 
DVSHVLKTWR NGEGSWGQEI KQIYPLEGNW KNLAPAEQSY QVGRCIGFLY GDLAYRKSTH 

      1390       1400       1410       1420       1430       1440 
AEDSSLFPLS IQNRVRGRGF LKGLLDGLMR ASCCQVIHRR SLAHLKRPAN AVYGGLIYLI 

      1450       1460       1470       1480       1490       1500 
DKLSVSPPFL SLTRSGPIRD ELETIPHKIP TSYPTSNRDM GVIVRNYFKY QCRLIEKGKY 

      1510       1520       1530       1540       1550       1560 
RSHYSQLWLF SDVLSIDFLG PFSISTTLLK ILYKSSLSGK DKNELRELAN LSSLLRSGEG 

      1570       1580       1590       1600       1610       1620 
WEDIHVKFFT KDILLCPEEI RHACKFGIAK DNNKDMSYPP WGRESRGTIT TIPVYYTATH 

      1630       1640       1650       1660       1670       1680 
YPKMLEMPPR IQNPLLSGIR LGQLPTGAHY KIRSILHGMG IHYRDFLSCG DGSGGITAAL 

      1690       1700       1710       1720       1730       1740 
LRENVHSRGI FNSLLELSGS VMRGASPEPP SALETLGGDK SRCVNGETCW EHPSDLCDPR 

      1750       1760       1770       1780       1790       1800 
TWDYFLRLKA GLGLQIDLIV MDMEVRDYSA SLKIETNVRN YVHRILDEQG VLIYKTYGTY 

      1810       1820       1830       1840       1850       1860 
ICESEKNAVT ILGPMFRTVD LVQTEFSSSQ TSEVYMVCKS LKKLIDEPHP DWSSVNESWK 

      1870       1880       1890       1900       1910       1920 
NLYAFQSSEQ EFARARKVST YFTLTGIPSQ FIPDPFVNLE TMLQIFGVPT GVSHAAALKS 

      1930       1940       1950       1960       1970       1980 
SDRPADLLTI GLFYMAIISY YNINHIRVGP VPPNPPSDGT AQNVGIAITG ISLWLSLMEK 

      1990       2000       2010       2020       2030       2040 
DIPLYQQCLA VIQQSFPIRW EAVSVKGGYK QKWSTRGDGL PKDTRISDSL APIGNWIRSL 

      2050       2060       2070       2080       2090       2100 
ELVRNQVRLN PFNEILFNQL CRTVDNHLRW SNLRKNTGMI EWVNRRISTE DRSILILKSD 


LHEENSWRD

Q8B0H0 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools