ID   DH12B_XENLA             Reviewed;         318 AA.
AC   Q8AVY8;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-NOV-2008, entry version 35.
DE   RecName: Full=Estradiol 17-beta-dehydrogenase 12-B;
DE            EC=1.1.1.62;
DE   AltName: Full=17-beta-hydroxysteroid dehydrogenase 12-B;
DE            Short=17-beta-HSD 12-B;
GN   Name=hsd17b12-B;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Amphibia; Batrachia; Anura; Mesobatrachia; Pipoidea; Pipidae;
OC   Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the transformation of estrone (E1) into
CC       estradiol (E2), suggesting a central role in estrogen formation
CC       (By similarity).
CC   -!- CATALYTIC ACTIVITY: Estradiol-17-beta + NAD(P)(+) = estrone +
CC       NAD(P)H.
CC   -!- PATHWAY: Steroid biosynthesis; estrogen biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC       membrane protein (By similarity).
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family. 17-beta-HSD 3 subfamily.
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DR   EMBL; BC041194; AAH41194.1; -; mRNA.
DR   RefSeq; NP_001080055.1; -.
DR   UniGene; Xl.21849; -.
DR   GeneID; 379747; -.
DR   KEGG; xla:379747; -.
DR   Xenbase; XB-FEAT-945228; hsd17b12.
DR   HOVERGEN; Q8AVY8; -.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004303; F:estradiol 17-beta-dehydrogenase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR002198; DHase_sc/Rdtase_SDR.
DR   InterPro; IPR002347; Glc/ribitol_DHase.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR19410; ADH_short_C2; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Lipid synthesis; Membrane; NADP;
KW   Oxidoreductase; Steroid biosynthesis; Transmembrane.
FT   CHAIN         1    318       Estradiol 17-beta-dehydrogenase 12-B.
FT                                /FTId=PRO_0000248374.
FT   TRANSMEM     15     35       Potential.
FT   TRANSMEM    187    207       Potential.
FT   TRANSMEM    281    301       Potential.
FT   NP_BIND      54     83       NADP (By similarity).
FT   ACT_SITE    207    207       Proton acceptor (By similarity).
FT   BINDING     194    194       Substrate (By similarity).
SQ   SEQUENCE   318 AA;  34861 MW;  A5D8971F8AE72AD0 CRC64;
     MAPESLAEVP GCNCFWYLGV VAATWWGLRA AWCLLNGARV WVLGSGAQVG PTIGKWAVVT
     GATDGIGKAY AEELARRGMN IVLISRSPEK LEEAAIHIKQ KFKVETKIIA ADFGKPTEIY
     ERIEAGLRDL EIGVLVNNVG ISYEYPEYFL EIPDLENTLD KMININIMSV CQMTRLVLPG
     MLGRGKGVVL NISSASGMYP VPLLTVYSAT KAFVDFFSRG LHAEYRSKGV TVQSVLPFFV
     ATKLAKIRKP TWDKPSPETY VRSALNTVGL QTQTNGYLPH AITGWISTSL VPVSAAISMG
     MKMNKGLRAR FLKKAKQN
//