NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=USDA 110;
DOI=10.1093/dnares/9.6.189; PubMed=12597275 [NCBI, ExPASy, EBI, Israel, Japan]
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110.";
DNA Res. 9:189-197(2002).

Comments

FUNCTION: Specifically methylates guanosine-37 in various tRNAs (By similarity).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N¹-methylguanine.
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (Potential).
SIMILARITY: Belongs to the RNA methyltransferase trmD family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BA000040; BAC45751.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_767126.1; -.

3D structure databases

HSSP

P43912; 1UAJ. [HSSP ENTRY / PDB]

ModBase

Q89X36.

Enzyme and pathway databases

BioCyc

BJAP224911:BLR0486-MON; -.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0003723;	Molecular function: RNA binding (inferred from electronic annotation from InterPro).
GO:0009019;	Molecular function: tRNA (guanine-N1-)-methyltransferase activity (inferred from electronic annotation from HAMAP).
GO:0006400;	Biological process: tRNA modification (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00605; -; 1.
PBIL [Tree]

InterPro

IPR016009; tRNA_m1G_MeTrfase.
IPR002649; tRNA_m1G_MeTrfase_bac.
Graphical view of domain structure.

Pfam

PF01746; tRNA_m1G_MT; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000386; tRNA_mtase; 1.

ProDom

PD004978; tRNA_m1G_mtfrase; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGR00088; trmD; 1.

Genome annotation databases

GeneID

1049220; -.

GenomeReviews

BA000040_GR; blr0486.

KEGG

bja:blr0486; -.

NMPDR

fig|224911.1.peg.486; -.

Phylogenomic databases

HOGENOM

Q89X36; -.

Genome annotation databases

CMR

Q89X36; blr0486.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; Methyltransferase; S-adenosyl-L-methionine; Transferase; tRNA processing.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 255`	`255`	`tRNA (guanine-N(1)-)-methyltransferase.`	`PRO_0000060341`
`REGION`	`141 146`	`6`	`S-adenosyl-L-methionine binding (By similarity).`
`BINDING`	`121 121`		`S-adenosyl-L-methionine; via amide nitrogen (By similarity).`

Sequence information

Length: 255 AA [This is the length of the unprocessed precursor]

Molecular weight: 27151 Da [This is the MW of the unprocessed precursor]

CRC64: 399DE5FC0FC9599D [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTTSSPWRAT VLTLFPEMFP GPLGVSLAGR ALASGLWQLE ARDIRASATD RHRSVDDTPA 

        70         80         90        100        110        120 
GGGPGMVLRA DVLAAAIDAA EIGQGSSKER PRLLMSPRGR PLTQARVVEL ARGPGPLIVC 

       130        140        150        160        170        180 
GRFEGIDQRV IDGRGLEEVS IGDYVLSGGE IAALALIDAC VRLLPGVMGK EASGTEESFS 

       190        200        210        220        230        240 
DGLLEYPQYT RPQLFEGVPI PEILTSGDHA KVAAWRRAEA EALTAARRPD LWAQIPVKAP 

       250 
NRAGRQKTPK NKTDG

Q89X36 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools