NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=USDA 110;
DOI=10.1093/dnares/9.6.189; PubMed=12597275 [NCBI, ExPASy, EBI, Israel, Japan]
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110.";
DNA Res. 9:189-197(2002).

Comments

FUNCTION: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA (By similarity).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N⁷-methylguanine.
SIMILARITY: Belongs to the methyltransferase superfamily. TrmB family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BA000040; BAC46053.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_767428.1; -.

3D structure databases

ModBase

Q89WA4.

Enzyme and pathway databases

BioCyc

BJAP224911:BLL0788-MON; -.

Ontologies

GO:0008176;	Molecular function: tRNA (guanine-N7-)-methyltransferase activity (inferred from electronic annotation from HAMAP).
GO:0006400;	Biological process: tRNA modification (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01057; -; 1.
PBIL [Tree]

InterPro

IPR003358; MeTrfase_4.
Graphical view of domain structure.

PANTHER

PTHR23417:SF1; Methyltransf_4; 1.

Pfam

PF02390; Methyltransf_4; 1.
Pfam graphical view of domain structure.

Genome annotation databases

GeneID

1051109; -.

GenomeReviews

BA000040_GR; bll0788.

KEGG

bja:bll0788; -.

NMPDR

fig|224911.1.peg.788; -.

Phylogenomic databases

HOGENOM

Q89WA4; -.

Genome annotation databases

CMR

Q89WA4; bll0788.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Methyltransferase; S-adenosyl-L-methionine; Transferase; tRNA processing.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 236`	`236`	`tRNA (guanine-N(7)-)-methyltransferase.`	`PRO_0000171304`
`BINDING`	`67 67`		`S-adenosyl-L-methionine (By similarity).`
`BINDING`	`92 92`		`S-adenosyl-L-methionine (By similarity).`
`BINDING`	`119 119`		`S-adenosyl-L-methionine (By similarity).`
`BINDING`	`141 141`		`S-adenosyl-L-methionine (By similarity).`
`BINDING`	`145 145`		`Substrate (By similarity).`
`BINDING`	`177 177`		`Substrate (Potential).`

Sequence information

Length: 236 AA [This is the length of the unprocessed precursor]

Molecular weight: 26766 Da [This is the MW of the unprocessed precursor]

CRC64: 09D1B7A43966F01C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSERKSDPDR DDSERAFFGR RKGHKLRQHQ AELIDHLLPH LALDIAGEAP GNACEIFDPA 

        70         80         90        100        110        120 
ASDVRLEIGF GGGEHLAAEA QNFATTGFIG CEPYVNGMAK ILAQIEAGNI ANIRLFAGDA 

       130        140        150        160        170        180 
AELLAWLPKA SLSRIDLIHP DPWPKRRHWK RRFVQDRTIA AMARVLKVGG EFRFVCDIDD 

       190        200        210        220        230 
YCAWTLSHLA RSADFKWLAE RADDFRQPWD GYTMTRYGRK AAREGRKAAY LRFRRS

Q89WA4 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools