NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=USDA 110;
DOI=10.1093/dnares/9.6.189; PubMed=12597275 [NCBI, ExPASy, EBI, Israel, Japan]
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110.";
DNA Res. 9:189-197(2002).

Comments

FUNCTION: Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate (By similarity).
CATALYTIC ACTIVITY: ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate.
PATHWAY: Cofactor biosynthesis; pantothenate biosynthesis; pantothenate from beta-alanine and pantoate: step 1/1 .
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (Potential).
MISCELLANEOUS: The reaction proceeds by a bi uni uni bi ping pong mechanism (By similarity).
SIMILARITY: Belongs to the pantothenate synthetase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BA000040; BAC50427.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_771802.1; -.

3D structure databases

HSSP

O06280; 1N2E. [HSSP ENTRY / PDB]

ModBase

Q89JV7.

Enzyme and pathway databases

BioCyc

BJAP224911:BLR5162-MON; -.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0005524;	Molecular function: ATP binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004592;	Molecular function: pantoate-beta-alanine ligase activity (inferred from electronic annotation from HAMAP).
GO:0015940;	Biological process: pantothenate biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00158; -; 1.
PBIL [Tree]

InterPro

IPR003721; Pantoate_ligase.
IPR014729; Rossmann-like_a/b/a_fold.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.

PANTHER

PTHR21299:SF1; Pantoate_ligase; 1.

Pfam

PF02569; Pantoate_ligase; 1.
Pfam graphical view of domain structure.

TIGR00018; panC; 1.

Genome annotation databases

GeneID

1051570; -.

GenomeReviews

BA000040_GR; blr5162.

KEGG

bja:blr5162; -.

NMPDR

fig|224911.1.peg.5162; -.

Phylogenomic databases

HOGENOM

Q89JV7; -.

Genome annotation databases

CMR

Q89JV7; blr5162.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Pantothenate biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 283`	`283`	`Pantothenate synthetase 2.`	`PRO_0000305409`
`NP_BIND`	`34 41`	`8`	`ATP (By similarity).`
`NP_BIND`	`152 155`	`4`	`ATP (By similarity).`
`NP_BIND`	`189 192`	`4`	`ATP (By similarity).`
`ACT_SITE`	`41 41`		`Proton donor (By similarity).`
`BINDING`	`65 65`		`Beta-alanine (By similarity).`
`BINDING`	`65 65`		`Pantoate (By similarity).`
`BINDING`	`158 158`		`Pantoate (By similarity).`
`BINDING`	`181 181`		`ATP; via amide nitrogen and carbonyl oxygen (By similarity).`

Sequence information

Length: 283 AA [This is the length of the unprocessed precursor]

Molecular weight: 30969 Da [This is the MW of the unprocessed precursor]

CRC64: 3EFFFB820731629C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSRSPLIART VPALRRAADN LRKRKATIAL VPTMGALHDG HVSLVRLAKR RASRVVVSIF 

        70         80         90        100        110        120 
VNPTQFAPTE DFGAYPRTWK ADIAKLAAED VDIVWHPGVE AMYPEGFATR IVPEGPALAG 

       130        140        150        160        170        180 
LEDRFRPHFF GGVATVVGKL FTQCRPDFAI FGEKDFQQLR VVTQMARDLD LGVKVIGSRT 

       190        200        210        220        230        240 
VRERDGLAMS SRNVYLSPQE RQTATTLYRA MKDSAGRIRA GEAIASAMAR GAATIKAAGF 

       250        260        270        280 
VLDYFEARHA ETLAQVTSRK DGPLRILVAA KLGTTRLIDN IAV

Q89JV7 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools