ID   DDLA_BRAJA              Reviewed;         373 AA.
AC   Q89GA7;
DT   15-DEC-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-NOV-2008, entry version 42.
DE   RecName: Full=D-alanine--D-alanine ligase A;
DE            EC=6.3.2.4;
DE   AltName: Full=D-alanylalanine synthase A;
DE   AltName: Full=D-Ala-D-Ala ligase A;
GN   Name=ddlA; OrderedLocusNames=blr6438;
OS   Bradyrhizobium japonicum.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=375;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=USDA 110;
RX   MEDLINE=22484998; PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T.,
RA   Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K.,
RA   Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M.,
RA   Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: Cell wall formation (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + 2 D-alanine = ADP + phosphate + D-
CC       alanyl-D-alanine.
CC   -!- COFACTOR: Binds 2 magnesium or manganese ions per subunit (By
CC       similarity).
CC   -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the D-alanine--D-alanine ligase family.
CC   -!- SIMILARITY: Contains 1 ATP-grasp domain.
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DR   EMBL; BA000040; BAC51703.1; -; Genomic_DNA.
DR   RefSeq; NP_773078.1; -.
DR   HSSP; P07862; 1IOW.
DR   GeneID; 1047518; -.
DR   GenomeReviews; BA000040_GR; blr6438.
DR   KEGG; bja:blr6438; -.
DR   NMPDR; fig|224911.1.peg.6438; -.
DR   HOGENOM; Q89GA7; -.
DR   BioCyc; BJAP224911:BLR6438-MON; -.
DR   GO; GO:0005618; C:cell wall; IEA:InterPro.
DR   GO; GO:0005737; C:cytoplasm; IEA:HAMAP.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0008716; F:D-alanine-D-alanine ligase activity; IEA:HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:HAMAP.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR   HAMAP; MF_00047; -; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013816; ATP_grasp_subdomain_2.
DR   InterPro; IPR000291; D-Ala_lig_Van_CS.
DR   InterPro; IPR005905; D_ala_D_ala.
DR   InterPro; IPR011095; Dala_Dala_lig_C.
DR   InterPro; IPR011127; Dala_Dala_lig_N.
DR   InterPro; IPR013817; Pre-ATP_grasp.
DR   Gene3D; G3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1.
DR   Gene3D; G3DSA:3.40.50.20; Pre-ATP_grasp; 1.
DR   Pfam; PF07478; Dala_Dala_lig_C; 1.
DR   Pfam; PF01820; Dala_Dala_lig_N; 1.
DR   TIGRFAMs; TIGR01205; D_ala_D_alaTIGR; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
DR   PROSITE; PS00843; DALA_DALA_LIGASE_1; 1.
DR   PROSITE; PS00844; DALA_DALA_LIGASE_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell shape; Cell wall biogenesis/degradation;
KW   Complete proteome; Cytoplasm; Ligase; Magnesium; Manganese;
KW   Metal-binding; Nucleotide-binding; Peptidoglycan synthesis.
FT   CHAIN         1    373       D-alanine--D-alanine ligase A.
FT                                /FTId=PRO_0000177792.
FT   DOMAIN      146    355       ATP-grasp.
FT   NP_BIND     179    234       ATP (By similarity).
FT   METAL       308    308       Magnesium or manganese 1 (By similarity).
FT   METAL       322    322       Magnesium or manganese 1 (By similarity).
FT   METAL       322    322       Magnesium or manganese 2 (By similarity).
FT   METAL       324    324       Magnesium or manganese 2 (By similarity).
SQ   SEQUENCE   373 AA;  40799 MW;  896E99143E48A84C CRC64;
     MADKIRVVVL YGGRSGEHEV SLKSAASVFR HLDRTRFEVI PVSIDKTGRW QWNDLRSLDQ
     AHAAALPILP DAPEMRLARG PDGRGVLVPI TQGAAAPIAI DVVFPVIHGP LCEDGTVQGL
     LELADVAYVG SGVLASAVSM DKDVAKRLAE FAGIPVAPYR VLTRKAFVQD RVSSLAKAVE
     GLSLPVFVKP CNMGSSVGIH KVKTQDALEA ALDDAFRYDV KVLVQQGIDA REIEVAVLED
     ETLFASLASE LNPNAHHEFY SYEAKYLDPD GARVDLPARL DAAQMERVRS LATRVFAALE
     CSGFARVDFF LDRKTGEFCF NEINTLPGFT SISMYPKMME ASGVPYGELL SRLVDLALDR
     HRQRQSLERG YAS
//