NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=USDA 110;
DOI=10.1093/dnares/9.6.189; PubMed=12597275 [NCBI, ExPASy, EBI, Israel, Japan]
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110.";
DNA Res. 9:189-197(2002).

Comments

FUNCTION: Provides the sole de novo source of dTMP for DNA biosynthesis (By similarity).
CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.
PATHWAY: Pyrimidine metabolism; dTTP biosynthesis .
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the thymidylate synthase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BA000040; BAC51777.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_773152.1; -.

3D structure databases

HSSP

P00470; 1TYS. [HSSP ENTRY / PDB]

ModBase

Q89G35.

Enzyme and pathway databases

BioCyc

BJAP224911:BLL6512-MON; -.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0004799;	Molecular function: thymidylate synthase activity (inferred from electronic annotation from HAMAP).
GO:0006231;	Biological process: dTMP biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00008; -; 1.
PBIL [Tree]

InterPro

IPR000398; Thymidylat_synth_C.
Graphical view of domain structure.

Gene3D

G3DSA:3.30.572.10; Thymidylat_synth_C; 1.

PANTHER

PTHR11549:SF2; Thymidylat_synth_C; 1.

Pfam

PF00303; Thymidylat_synt; 1.
Pfam graphical view of domain structure.

PRINTS

PR00108; THYMDSNTHASE.

ProDom

PD001180; Thymidylat_synth; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGRFAMs

TIGR03284; thym_sym; 1.

PROSITE

PS00091; THYMIDYLATE_SYNTHASE; 1.

Genome annotation databases

GeneID

1054597; -.

GenomeReviews

BA000040_GR; bll6512.

KEGG

bja:bll6512; -.

NMPDR

fig|224911.1.peg.6512; -.

Phylogenomic databases

HOGENOM

Q89G35; -.

Genome annotation databases

CMR

Q89G35; bll6512.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; Methyltransferase; Nucleotide biosynthesis; Transferase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 264`	`264`	`Thymidylate synthase.`	`PRO_0000140941`
`ACT_SITE`	`146 146`		`By similarity.`

Sequence information

Length: 264 AA [This is the length of the unprocessed precursor]

Molecular weight: 29941 Da [This is the MW of the unprocessed precursor]

CRC64: 112056C0A989073F [This is a checksum on the sequence]

        10         20         30         40         50         60 
MHQYQDLLER ILSDGAEKTD RTGTGTLSVF GHQMRFNLSA GFPMLTTKRL PLKAIVHELL 

        70         80         90        100        110        120 
WFLKGDTNIK YLRDNGVTIW DEWADANGDL GPVYGHQWRS WPAPDGRSID QIANVVDMIK 

       130        140        150        160        170        180 
RNPDSRRLIV SAWNPAEVDK MALPPCHCLF QFYVANGKLS CQLYQRSADV FLGVPFNIAS 

       190        200        210        220        230        240 
YALLTMMVAQ VTGLKPGDFV HSFGDTHLYS NHLEQAKLQL TRAPRALPVM RINPDVKDIF 

       250        260 
SFRFEDFELV GYDPHPHIKA EVAV

Q89G35 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools