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UniProtKB/Swiss-Prot entry Q89330

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name POLG_ZYMVR
Primary accession number Q89330
Secondary accession numbers None
Integrated into Swiss-Prot on December 15, 1998
Sequence was last modified on November 1, 1996 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 73)
Name and origin of the protein
Protein name Genome polyprotein
Synonyms None
Contains P1 proteinase
     (N-terminal protein)
Helper component proteinase
     (HC-pro)
     (EC 3.4.22.45)
Protein P3
6 kDa protein 1
     (6K1)
Cytoplasmic inclusion protein
     (CI)
     (EC 3.6.1.-)
6 kDa protein 2
     (6K2)
Viral genome-linked protein
     (VPg)
Nuclear inclusion protein A
     (NI-a)
     (NIa)
     (EC 3.4.22.44)
     (NIa-pro)
     (49 kDa proteinase)
     (49 kDa-Pro)
Nuclear inclusion protein B
     (NI-b)
     (NIb)
     (EC 2.7.7.48)
     (RNA-directed RNA polymerase)
Coat protein
     (CP)
Gene name None
From
Zucchini yellow mosaic virus (strain Reunion Island) (ZYMV) [TaxID: 117129] 
Taxonomy Viruses; ssRNA positive-strand viruses, no DNA stage; Potyviridae; Potyvirus.
Virus hosts Citrullus lanatus (Watermelon) (Citrullus vulgaris) [TaxID: 3654]
Cucumis melo (Muskmelon) [TaxID: 3656]
Cucumis sativus (Cucumber) [TaxID: 3659]
Cucurbita pepo (Vegetable marrow) (Summer squash) [TaxID: 3663]
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC RNA].
PubMed=7844540 [NCBI, ExPASy, EBI, Israel, Japan]
Wisler G.C., Purcifull D.E., Hiebert E.;
"Characterization of the P1 protein and coding region of the zucchini yellow mosaic virus.";
J. Gen. Virol. 76:37-45(1995).
[2]
 REVIEW.
DOI=10.1016/S0168-1702(01)00220-9; PubMed=11226583 [NCBI, ExPASy, EBI, Israel, Japan]
Urcuqui-Inchima S., Haenni A.L., Bernardi F.;
"Potyvirus proteins: a wealth of functions.";
Virus Res. 74:157-175(2001).
Comments
  • FUNCTION: Coat protein is involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification.
  • FUNCTION: Nuclear inclusion protein B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication.
  • FUNCTION: Helper component proteinase is required for aphid transmission and also has proteolytic activity. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity (By similarity).
  • FUNCTION: Cytoplasmic inclusion protein has helicase activity. It may be involved in replication (By similarity).
  • FUNCTION: Both 6K peptides are indispensable for virus replication (By similarity).
  • FUNCTION: Nuclear inclusion protein A has RNA-binding and proteolytic activities.
  • CATALYTIC ACTIVITY: Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.
  • CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
  • CATALYTIC ACTIVITY: Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.
  • SUBCELLULAR LOCATION: Coat protein: Virion (Potential).
  • DOMAIN: The N-terminus of helper component proteinase is involved in interaction with stylets. The central part is involved in interaction with virions and the C-terminus is involved in cell-to cell movement of the virus.
  • PTM: VPg is covalently linked to the genomic RNA (By similarity).
  • PTM: The viral RNA of potyviruses is expressed as a single polyprotein which undergoes post-translational proteolytic processing by the main proteinase NIa-pro resulting in the production of at least ten individual proteins. The P1 proteinase and the HC-pro cleave only their respective C-termini autocatalytically. 6K1 is essential for proper proteolytic separation of P3 from CI (By similarity).
  • SIMILARITY: Belongs to the potyviruses polyprotein family.
  • SIMILARITY: Contains 1 helicase ATP-binding domain.
  • SIMILARITY: Contains 1 helicase C-terminal domain.
  • SIMILARITY: Contains 1 peptidase C4 domain [view classification].
  • SIMILARITY: Contains 1 peptidase C6 domain [view classification].
  • SIMILARITY: Contains 1 RdRp catalytic domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
L29569; AAA65558.1; -; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
3D structure databases
HSSP P27958; 1HEI. [HSSP ENTRY / PDB]
ModBase Q89330.
Ontologies
GO
GO:0019028; Cellular component: viral capsid (inferred from electronic annotation from InterPro).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from InterPro).
GO:0008026; Molecular function: ATP-dependent helicase activity (inferred from electronic annotation from InterPro).
GO:0004197; Molecular function: cysteine-type endopeptidase activity (inferred from electronic annotation from InterPro).
GO:0003723; Molecular function: RNA binding (inferred from electronic annotation from InterPro).
GO:0003968; Molecular function: RNA-directed RNA polymerase activity (inferred from electronic annotation from InterPro).
GO:0005198; Molecular function: structural molecule activity (inferred from electronic annotation from UniProtKB-KW).
GO:0006508; Biological process: proteolysis (inferred from electronic annotation from InterPro).
GO:0018144; Biological process: RNA-protein covalent cross-linking (inferred from electronic annotation from UniProtKB-KW).
GO:0006410; Biological process: transcription, RNA-dependent (inferred from electronic annotation from UniProtKB-KW).
GO:0019079; Biological process: viral genome replication (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR014001; DEAD-like_N.
IPR001650; DNA/RNA_helicase_C.
IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
IPR014021; Helicase_SF1/SF2_ATP-bd.
IPR002540; Pept_S30_P1_potyvir.
IPR001730; Peptidase_C4.
IPR001456; Peptidase_C6.
IPR001592; Poty_coat.
IPR013648; PP_Potyviridae.
IPR001205; RNA_pol_P3D.
IPR007094; RNA_pol_PSvir.
Graphical view of domain structure.
Pfam PF00270; DEAD; 1.
PF00271; Helicase_C; 1.
PF00863; Peptidase_C4; 1.
PF00851; Peptidase_C6; 1.
PF01577; Peptidase_S30; 1.
PF00767; Poty_coat; 1.
PF08440; Poty_PP; 1.
PF00680; RdRP_1; 1.
Pfam graphical view of domain structure.
PRINTS PR00966; NIAPOTYPTASE.
SMART SM00487; DEXDc; 1.
SM00490; HELICc; 1.
SMART graphical view of domain structure.
PROSITE PS51192; HELICASE_ATP_BIND_1; 1.
PS51194; HELICASE_CTER; 1.
PS50507; RDRP_SSRNA_POS; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q89330.
ProtoNet Q89330.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Capsid protein; Complete proteome; Covalent protein-RNA linkage; Helicase; Hydrolase; Nucleotide-binding; Nucleotidyltransferase; Phosphoprotein; Protease; RNA replication; RNA-directed RNA polymerase; Suppressor of RNA silencing; Thiol protease; Transferase; Virion.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1    314  314     P1 proteinase (Potential). PRO_0000040510
CHAIN   315    769  455     Helper component proteinase (Potential). PRO_0000040511
CHAIN   770   1115  346     Protein P3 (By similarity). PRO_0000040512
CHAIN   1116   1167  52     6 kDa protein 1 (By similarity). PRO_0000040513
CHAIN   1168   1801  634     Cytoplasmic inclusion protein (By similarity). PRO_0000040514
CHAIN   1802   1854  53     6 kDa protein 2 (By similarity). PRO_0000040515
CHAIN   1855   2044  190     Viral genome-linked protein (By similarity). PRO_0000040516
CHAIN   2045   2287  243     Nuclear inclusion protein A (By similarity). PRO_0000040517
CHAIN   2288   2804  517     Nuclear inclusion protein B (By similarity). PRO_0000040518
CHAIN   2805   3083  279     Coat protein (By similarity). PRO_0000040519
DOMAIN   1239   1391  153     Helicase ATP-binding. 
DOMAIN   1410   1569  160     Helicase C-terminal. 
DOMAIN   2529   2653  125     RdRp catalytic. 
NP_BIND   1252   1259  8     ATP (Potential). 
MOTIF   365    368  4     Involved in interaction with stylet and aphid transmission (By similarity). 
MOTIF   621    623  3     Involved in virions binding and aphid transmission (By similarity). 
MOTIF   1341   1344  4     DECH box. 
MOTIF   1894   1903  10     Nuclear localization signal (Potential). 
ACT_SITE   655    655        For helper component proteinase activity (By similarity). 
ACT_SITE   728    728        For helper component proteinase activity (By similarity). 
ACT_SITE   2090   2090        For nuclear inclusion protein A activity (By similarity). 
ACT_SITE   2125   2125        For nuclear inclusion protein A activity (By similarity). 
ACT_SITE   2195   2195        For nuclear inclusion protein A activity (By similarity). 
SITE   314    315  2     Cleavage; by P1 proteinase (Potential). 
SITE   769    770  2     Cleavage; by HC-pro (Potential). 
SITE   1115   1116  2     Cleavage; by NIa-pro (By similarity). 
SITE   1167   1168  2     Cleavage; by NIa-pro (By similarity). 
SITE   1801   1802  2     Cleavage; by NIa-pro (By similarity). 
SITE   2044   2045  2     Cleavage; by NIa-pro (By similarity). 
SITE   2287   2288  2     Cleavage; by NIa-pro (By similarity). 
SITE   2804   2805  2     Cleavage; by NIa-pro (By similarity). 
MOD_RES   1918   1918        O-(5'-phospho-RNA)-tyrosine (By similarity). 
Sequence information
Length: 3083 AA [This is the length of the unprocessed precursor] Molecular weight: 351161 Da [This is the MW of the unprocessed precursor] CRC64: 55E51B455C20C537 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MAAIMIGSIS VPIVESARCA TVQTGNRVNI VAPGHVAVCK PQMKSHSYYK HASEKLSKQA 

        70         80         90        100        110        120 
SESINILNSF FDTDPEMRFR LTRNEMSKVK KGPNGRMILR KPRAQRVLER ISFEKIEKGA 

       130        140        150        160        170        180 
ERQVLPWRVY ATVTSIINTF TDERNGIANS SLRSPFYKRS CRKEKKKIVC ENVVRSASVN 

       190        200        210        220        230        240 
NLCDRVLKIA REKNIPVEMI GKKKNRHTLT FKNFKGSFIG KVSLAHERGQ MRHVEMSYEQ 

       250        260        270        280        290        300 
FGFILQAICR VTNTRCVRDE DIKPGCSGWV LGDDHELTQK FSRLPCLVIR GRDDEGIVNA 

       310        320        330        340        350        360 
LEPVFFYDDV DHYSSQPEVQ FFQGWRRMFD NFKPSSDHVC KVDHGNEECG ELAAIFSQAL 

       370        380        390        400        410        420 
FPVVKLSCQT CREKLSRVSF EEFKDSLAIN FTVHKSEWDS LKENPHHDNV LKLIKGATQA 

       430        440        450        460        470        480 
TQNLKLSSEV MKLVQNHTST HMKQIQDINR ALMKGSLVTQ DELDLALKQL LEMTQWFKNH 

       490        500        510        520        530        540 
MHLTGEEALK TFRNKRSSKA MINPSLLCDN QLDKNGNFVW GERGYHSKRL FKNFFEEVIP 

       550        560        570        580        590        600 
SEGYTKYIVR NFPNGTRKLA IGSLIVPLNL DRARTALLGE SIEKEPLTSA CVSQQNGNYI 

       610        620        630        640        650        660 
HSCCCVTMDD GTPMYSDVKS PTKRHLVIGA SGDPKYIDLP ASEADRMYIA KEGYCYLNIF 

       670        680        690        700        710        720 
LAMLVNVNEN EAKDFTKMIR DVLIPMLGQW PSLMDVATAA YILGVFHPET RCAELPRILV 

       730        740        750        760        770        780 
DHATQTMHVI DSYGSLTVGY HVLKAGTVNH LIQFASNDLQ SEMKHYRVGG TPTQRIKLEE 

       790        800        810        820        830        840 
QLIKGIFKPK LMMQLLQDDP YVLILGMVSP TILVHMYRMR HFERGIEMWI KRDHEVGKIF 

       850        860        870        880        890        900 
VILEQLTRKV ALTEVLVDQL DLISEASPHL LEIMKGCQDN QRAYVPALDL LTVQVEREFS 

       910        920        930        940        950        960 
NKELKVNGYP DLQQTLYDMR EKIYAKQLHD SWQELSLLEK SCVTVRLKRF SIFTERKLTQ 

       970        980        990       1000       1010       1020 
QAKDGKRVSS LQFVHECFIT TRVHAKSIRD VGMRKFNEAL VGTCKFLFTC GFKIFARCYS 

      1030       1040       1050       1060       1070       1080 
DIIYLVNVCL IFSLVLQMSN TVRSMISATR EEKERALANK ADENERTLMH MYHIFSKKQD 

      1090       1100       1110       1120       1130       1140 
DAPLYSEFLE HVRNVRPDLE ETLLYMAGAE IVTPQAKSAV QVQFEKIIAV VALLTMCFDA 

      1150       1160       1170       1180       1190       1200 
ERSDAIFKIL TKLKTVFGTV GETVRLQGLE DIENLEDDKK LTIDFDINTN EAQSSTTFDV 

      1210       1220       1230       1240       1250       1260 
HFDDWWNRQL QQNRTVPHYR TTGKFLEFTR NTAAFVANEI ASSNEGEFLV RGAVGSGKST 

      1270       1280       1290       1300       1310       1320 
SLPAHLAKKG KVLLLEPTRP LAENVSRQLA GDPFFQNVTL RMRGLSCFGS SNITVMTSGY 

      1330       1340       1350       1360       1370       1380 
AFHYYVNNPH QMMEFDFIII DECHVTDSAT IAFNCALKEY NFAGKLIKVS ATPPGRECDF 

      1390       1400       1410       1420       1430       1440 
DTQFAVKVKT EDHLSFQAFV GAQRTGSNAD MIQHGNNILV YVASYNEVDM LSKLLTERQF 

      1450       1460       1470       1480       1490       1500 
SVTKVDGRTM QLGKTTIETH GTSQKPHFIV ATNIIENGVT LDVDCVVDFG LKVVAELDSE 

      1510       1520       1530       1540       1550       1560 
SRCVRYSKKP VNYGERIQRL GRVGRSKPGT ALRIGHTEKG IENIPEFIAT EAAALSFAYG 

      1570       1580       1590       1600       1610       1620 
LSVTTHGVST NILGKCTVKQ MKCALNFELT PFFTTHLIRH DGSMHPLIHE ELKQFKLRDS 

      1630       1640       1650       1660       1670       1680 
EMVLNKVALP HQFVSQWMTQ GDYEHIGVHI QCNENTRIPF YTNGIPDRVY EKIWKCIQEN 

      1690       1700       1710       1720       1730       1740 
KNDALFGRLS SACSTKVSYT LSTDPAALPR TIAIIDHLLA EEMMKRNHFD MISSAVTGYS 

      1750       1760       1770       1780       1790       1800 
FSLAGIADSF RKRYMRDHTA HNIAILQQAR AQLLEFDSKN VNINNLSDLE GIGVIKSVVL 

      1810       1820       1830       1840       1850       1860 
QSKQEVSNFL GLRGKWDGRK FANDVILAIM TLLGGGWFMW EYFTKKVNEP VRVESKKRRS 

      1870       1880       1890       1900       1910       1920 
QKLKFRDAYD RKVGREIFGD DETIGRTFGE AYTKRGKVKG NNSTKGMGRK TRNFVHLYGV 

      1930       1940       1950       1960       1970       1980 
EPENYSFIRF VDPLTGHTLD ESTHTDISLV QEEFGNIREK FLENDLISRQ SIINKPGIQA 

      1990       2000       2010       2020       2030       2040 
YFMGKGTAEA LKFDFTPHVP SLSCSNSNAH AGYPERENEL RQTGTPVKVS LKDVPEKNEH 

      2050       2060       2070       2080       2090       2100 
VELESKSIYK GVRDYNGIST IVCQLTNDSD RLKETMYGIG YGPIIITNGH LFRKNNGTIL 

      2110       2120       2130       2140       2150       2160 
VRSWHGEFTV KNTTTLKVHF IEGKDVVLVR MPKTFPPFKS NASFRAPKRE ERACLVGTNF 

      2170       2180       2190       2200       2210       2220 
QEKSLRSTVS ESSMTNPRRT GSYWIHWIST NEGDCGLPMV STTDGKLIGL HGKASTVSSK 

      2230       2240       2250       2260       2270       2280 
NYFVPFTDDF MATHLSKLDD LTWTQHWLWQ PSKIAWGLLN LVDEQPGPEF RISNLVKDLF 

      2290       2300       2310       2320       2330       2340 
NSGVETQSKR ERWVYESCEG NLRAVGSAQS ALVTKHVVKG KCPFFEEYLQ THAEASAYFR 

      2350       2360       2370       2380       2390       2400 
PLMGEYQPSK LNKEAFKKDF FKYNKPVIVN QLDHDKFLEA VDGVIRMMCD FEFNECRFIT 

      2410       2420       2430       2440       2450       2460 
DPEEIYSSLN MKAAIGAQYR GKKKEYFEGL DNFDMERLLF QSCERLFNGY KGLWNGSLKA 

      2470       2480       2490       2500       2510       2520 
ELRPLEKVQA NKTRTFTAAP IDTLLGAKVC VDDFNNEFYS KNLKCPWTVG MSKFYGGWDK 

      2530       2540       2550       2560       2570       2580 
LMRALPDGWL YCHADGSQFD SSLTPALLNA VLIIRSFYME DWWVGQEMLE NLYAEIVYTP 

      2590       2600       2610       2620       2630       2640 
ILAPDGTIFK KFRGNNSGQP STVVDNTLMV VISIYYACIK FGWGYDEIEN RLVFFANGDD 

      2650       2660       2670       2680       2690       2700 
LILAVKDEDS GLLDNMSASF SELGLNYDFS ERTHKREDLW FMSHQAMLVD GMYIPKLERE 

      2710       2720       2730       2740       2750       2760 
RIVSILEWDR SKEVMHRTEA ICAAMIEAWG HTELLHEIRK FYLWFVEKEE VRELAALGKA 

      2770       2780       2790       2800       2810       2820 
PYIAETALRK LYTDKGAETS ELARYLQALH QDVFFEQRDT VMLQSDTQTK EADAGAAKRD 

      2830       2840       2850       2860       2870       2880 
KDEEKEKKKD VASSSANEKT MTATAKDKDV NAGSHGKIVP RLSKITKKMS LPRVKGSVIL 

      2890       2900       2910       2920       2930       2940 
DIDHLLEYKP DQIELYNTRA SHQQFASWFN QVKAEYDLNE QQMGVVMNGF MVWCIENGTS 

      2950       2960       2970       2980       2990       3000 
PDINGVWFMM DGDEQVEYPL KPIVENAKPT LRQIMHHFSD AAEAYIEMRN AEAPYMPRYG 

      3010       3020       3030       3040       3050       3060 
LLRNLRDRSL ARYAFDFYEV NSKTPDRARE AVAQMKAAAL SNVSSRLFGL DGNVATNSED 

      3070       3080 
TERHTARDVN RNMHTLLGVN TMQ
Q89330 in FASTA format

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