ID   DDLA_PSEPK              Reviewed;         352 AA.
AC   Q88EV6;
DT   15-DEC-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-NOV-2008, entry version 42.
DE   RecName: Full=D-alanine--D-alanine ligase A;
DE            EC=6.3.2.4;
DE   AltName: Full=D-alanylalanine synthetase A;
DE   AltName: Full=D-Ala-D-Ala ligase A;
GN   Name=ddlA; OrderedLocusNames=PP_4346;
OS   Pseudomonas putida (strain KT2440).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=160488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22423060; PubMed=12534463;
RX   DOI=10.1046/j.1462-2920.2002.00366.x;
RA   Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA   Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA   Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA   Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M.,
RA   Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K.,
RA   Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D.,
RA   Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S.,
RA   Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B.,
RA   Fraser C.M.;
RT   "Complete genome sequence and comparative analysis of the
RT   metabolically versatile Pseudomonas putida KT2440.";
RL   Environ. Microbiol. 4:799-808(2002).
CC   -!- FUNCTION: Cell wall formation (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + 2 D-alanine = ADP + phosphate + D-
CC       alanyl-D-alanine.
CC   -!- COFACTOR: Binds 2 magnesium or manganese ions per subunit (By
CC       similarity).
CC   -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the D-alanine--D-alanine ligase family.
CC   -!- SIMILARITY: Contains 1 ATP-grasp domain.
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DR   EMBL; AE015451; AAN69925.1; -; Genomic_DNA.
DR   RefSeq; NP_746461.1; -.
DR   HSSP; P07862; 1IOW.
DR   GeneID; 1042642; -.
DR   GenomeReviews; AE015451_GR; PP_4346.
DR   KEGG; ppu:PP_4346; -.
DR   NMPDR; fig|160488.1.peg.4291; -.
DR   TIGR; PP_4346; -.
DR   HOGENOM; Q88EV6; -.
DR   BioCyc; PPUT160488:PP_4346-MON; -.
DR   GO; GO:0005618; C:cell wall; IEA:InterPro.
DR   GO; GO:0005737; C:cytoplasm; IEA:HAMAP.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0008716; F:D-alanine-D-alanine ligase activity; IEA:HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:HAMAP.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR   HAMAP; MF_00047; -; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013816; ATP_grasp_subdomain_2.
DR   InterPro; IPR000291; D-Ala_lig_Van_CS.
DR   InterPro; IPR005905; D_ala_D_ala.
DR   InterPro; IPR011095; Dala_Dala_lig_C.
DR   InterPro; IPR011127; Dala_Dala_lig_N.
DR   InterPro; IPR013817; Pre-ATP_grasp.
DR   Gene3D; G3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1.
DR   Gene3D; G3DSA:3.40.50.20; Pre-ATP_grasp; 1.
DR   Pfam; PF07478; Dala_Dala_lig_C; 1.
DR   Pfam; PF01820; Dala_Dala_lig_N; 1.
DR   TIGRFAMs; TIGR01205; D_ala_D_alaTIGR; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
DR   PROSITE; PS00843; DALA_DALA_LIGASE_1; 1.
DR   PROSITE; PS00844; DALA_DALA_LIGASE_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell shape; Cell wall biogenesis/degradation;
KW   Complete proteome; Cytoplasm; Ligase; Magnesium; Manganese;
KW   Metal-binding; Nucleotide-binding; Peptidoglycan synthesis.
FT   CHAIN         1    352       D-alanine--D-alanine ligase A.
FT                                /FTId=PRO_0000177857.
FT   DOMAIN      138    341       ATP-grasp.
FT   NP_BIND     165    220       ATP (By similarity).
FT   METAL       295    295       Magnesium or manganese 1 (By similarity).
FT   METAL       308    308       Magnesium or manganese 1 (By similarity).
FT   METAL       308    308       Magnesium or manganese 2 (By similarity).
FT   METAL       310    310       Magnesium or manganese 2 (By similarity).
SQ   SEQUENCE   352 AA;  37805 MW;  51FA8522FD896BB1 CRC64;
     MKQNVIALVF GGRSSEHSVA LRSAATIHAA LMALGHRVHC VGIDREGNWR YQGEPCQFPG
     AVDRSAPLIS IRPGHRSLSY TTQESGTVEI GIDLLFPALH GRWGEDGTIQ GLAAMCGLPC
     VGSGVLGSAM AMDKDVTKRM VQSAGLVVVP WLAMNSMRPW EELVECLGSS TLFVKPATSG
     SSIGVSRVSN ALEYAAAFAI AAREDTKVLV EAAVCGREIE CGVLELEEGL MASVVGEIIK
     KEGHAYYDYQ AKYDSNSVTG LRVPSLLPPD IVARIQALSV QAFRCLELKG YARVDFFLTE
     EGEIILNEIN TLPGFTSASM YPKMFECSGY PPPKLVGALV EYGLSSASKE RL
//