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UniProtKB/Swiss-Prot entry Q88D35

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GSHB_PSEPK
Primary accession number Q88D35
Secondary accession numbers None
Integrated into Swiss-Prot on October 31, 2003
Sequence was last modified on June 1, 2003 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 34)
Name and origin of the protein
Protein name Glutathione synthetase
Synonyms EC 6.3.2.3
Glutathione synthase
GSH synthetase
GSH-S
GSHase
Gene name
Name: gshB
OrderedLocusNames: PP_4993
From
Pseudomonas putida (strain KT2440) [TaxID: 160488] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1046/j.1462-2920.2002.00366.x; PubMed=12534463 [NCBI, ExPASy, EBI, Israel, Japan]
Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440.";
Environ. Microbiol. 4:799-808(2002).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE015451; AAN70559.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_747095.1; -.
3D structure databases
HSSP P04425; 1GSH. [HSSP ENTRY / PDB]
ModBase Q88D35.
Enzyme and pathway databases
BioCyc PPUT160488:PP_4993-MON; -.
Ontologies
GO
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from HAMAP).
GO:0004363; Molecular function: glutathione synthase activity (inferred from electronic annotation from HAMAP).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0030145; Molecular function: manganese ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006750; Biological process: glutathione biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.
Family and domain databases
HAMAP MF_00162; -; 1.
PBIL [Tree]
InterPro IPR011761; ATP-grasp.
IPR013816; ATP_grasp_subdomain_2.
IPR006284; Glut_synth_pro.
IPR004218; GSHS_ATP_bd.
IPR004215; GSHS_N.
IPR013817; Pre-ATP_grasp.
Graphical view of domain structure.
Gene3D G3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1.
G3DSA:3.40.50.20; Pre-ATP_grasp; 1.
Pfam PF02955; GSH-S_ATP; 1.
PF02951; GSH-S_N; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR01380; glut_syn; 1.
PROSITE PS50975; ATP_GRASP; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q88D35.
ProtoNet Q88D35.
Genome annotation databases
GeneID 1042200; -.
GenomeReviews AE015451_GR; PP_4993.
KEGG ppu:PP_4993; -.
NMPDR fig|160488.1.peg.4925; -.
TIGR PP_4993; -.
Phylogenomic databases
HOGENOM Q88D35; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Complete proteome; Glutathione biosynthesis; Ligase; Magnesium; Manganese; Metal-binding; Nucleotide-binding.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   317  317     Glutathione synthetase. PRO_0000197477
DOMAIN   125   311  187     ATP-grasp. 
NP_BIND   152   208  57     ATP (By similarity). 
METAL   282   282        Magnesium or manganese (By similarity). 
METAL   284   284        Magnesium or manganese (By similarity). 
Sequence information
Length: 317 AA [This is the length of the unprocessed precursor] Molecular weight: 35255 Da [This is the MW of the unprocessed precursor] CRC64: 9A731692A21C6E4F [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MSVRLGIVMD PIASISYKKD SSLAMLLAAQ ARGWSLFYME QQDLYQGEGK ARARMRPLKV 

        70         80         90        100        110        120 
FADPARWFEL GEEQDSPLAE LDVILMRKDP PFDMEFVYST YLLEQAENDG VLVVNRPQSL 

       130        140        150        160        170        180 
RDCNEKMFAT LFPQCTTPTL VSRRPDIIRE FTAKHADVIL KPLDGMGGTS IFRHRAGDPN 

       190        200        210        220        230        240 
LSVILETLTA LGTQQIMAQA YLPAIKDGDK RILMIDGEPV DYCLARIPAS GETRGNLAAG 

       250        260        270        280        290        300 
GRGEARPLTE RDRWIAAQVG PTLREKGLLF VGLDVIGDYL TEINVTSPTC IREIDAAYNT 

       310 
DIGGKLMDAI DRKLKAR
Q88D35 in FASTA format

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