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UniProtKB/Swiss-Prot entry Q86U44


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MTA70_HUMAN
Primary accession number Q86U44
Secondary accession numbers O14736 Q86V05 Q9HB32
Integrated into Swiss-Prot on July 25, 2003
Sequence was last modified on July 25, 2003 (Sequence version 2)
Annotations were last modified on    June 16, 2009 (Entry version 56)
Name and origin of the protein
Protein name N6-adenosine-methyltransferase 70 kDa subunit
Synonyms MT-A70
EC 2.1.1.62
Methyltransferase-like protein 3
Gene name
Name: METTL3
Synonyms: MTA70
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), PROTEIN SEQUENCE OF 48-56; 134-149 AND 509-522, FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
PubMed=9409616 [NCBI, ExPASy, EBI, Israel, Japan]
Bokar J.A., Shambaugh M.E., Polayes D., Matera A.G., Rottman F.M.;
"Purification and cDNA cloning of the AdoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase.";
RNA 3:1233-1247(1997).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
TISSUE=Placenta;
Li W.B., Gruber C., Jessee J., Polayes D.;
"Full-length cDNA libraries and normalization.";
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature01348; PubMed=12508121 [NCBI, ExPASy, EBI, Israel, Japan]
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H., Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.;
"The DNA sequence and analysis of human chromosome 14.";
Nature 421:601-607(2003).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
TISSUE=Lung, and Pancreas;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1038/nbt1240; PubMed=16964243 [NCBI, ExPASy, EBI, Israel, Japan]
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.;
"A probability-based approach for high-throughput protein phosphorylation analysis and site localization.";
Nat. Biotechnol. 24:1285-1292(2006).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-350 AND THR-356, AND MASS SPECTROMETRY.
DOI=10.1126/science.1140321; PubMed=17525332 [NCBI, ExPASy, EBI, Israel, Japan]
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.;
"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.";
Science 316:1160-1166(2007).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43; SER-219 AND SER-243, AND MASS SPECTROMETRY.
DOI=10.1073/pnas.0805139105; PubMed=18669648 [NCBI, ExPASy, EBI, Israel, Japan]
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.;
"A quantitative atlas of mitotic phosphorylation.";
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
[8]
IDENTIFICATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
Colinge J., Superti-Furga G., Bennett K.L.;
Submitted (OCT-2008) to UniProtKB.
Comments
  • FUNCTION: N6-methyltransferase that methylates adenosine residues of some mRNAs. N6-methyladenosine (m6A), which is present at internal sites of some mRNAs, may play a role in the efficiency of mRNA splicing, transport or translation.
  • CATALYTIC ACTIVITY: S-adenosyl-L-methionine + m7G(5')pppAm = S-adenosyl-L-homocysteine + m7G(5')pppm6Am.
  • SUBCELLULAR LOCATION: Nucleus speckle. Note=Colocalizes with speckles in interphase nuclei. Suggesting that it may be associated with nuclear pre-mRNA splicing components.
  • ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.
    Name1
    Isoform IDQ86U44-1
    This is the isoform sequence displayed in this entry.
    Name2
    Isoform IDQ86U44-2
    Note: No experimental confirmation available.
    Features which should be applied to build the isoform sequence: VSP_007864, VSP_007865, VSP_007866.
  • TISSUE SPECIFICITY: Widely expressed at low level. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
  • PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
  • SIMILARITY: Belongs to the MT-A70-like family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF014837; AAB71850.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF283991; AAG13956.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE000658; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
BX247964; CAD62303.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC003031; AAH03031.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC001650; AAH01650.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC052244; AAH52244.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00009755; -.
IPI00328636; -.
RefSeq NP_062826.2; -.
UniGene Hs.168799
3D structure databases
ModBase Q86U44.
PTM databases
PhosphoSite Q86U44; -.
Enzyme and pathway databases
BRENDA 2.1.1.62; 247.
Reactome REACT_1675; mRNA Processing.
REACT_71; Gene Expression.
Organism-specific databases
GeneCards GC14M021036; -.
H-InvDB HIX0011511; -.
HGNC HGNC:17563; METTL3.
GenAtlas METTL3.
HPA HPA001299; -.
MIM 612472; gene. [NCBI / EBI]
PharmGKB PA134955499; -.
Gene expression databases
Bgee Q86U44; -.
CleanEx HS_METTL3; -.
GermOnline ENSG00000165819; Homo sapiens.
Ontologies
GO
GO:0016607; Cellular component: nuclear speck (inferred from electronic annotation from UniProtKB-SubCell).
GO:0016422; Molecular function: mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity (inferred from mutant phenotype from UniProtKB).
GO:0003723; Molecular function: RNA binding (inferred from electronic annotation from UniProtKB-KW).
GO:0001510; Biological process: RNA methylation (inferred from mutant phenotype from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR007757; MT-A70.
Graphical view of domain structure.
Pfam PF05063; MT-A70; 1.
Pfam graphical view of domain structure.
PROSITE PS51143; MT_A70; 1.
PROSITE graphical view of domain structure (profiles).
Proteomic databases
PeptideAtlas Q86U44; -.
PRIDE Q86U44; -.
Genome annotation databases
Ensembl ENSG00000165819; Homo sapiens. [Contig view]
GeneID 56339; -.
KEGG hsa:56339; -.
Phylogenomic databases
HOGENOM Q86U44; -.
HOVERGEN Q86U44; -.
OMA Q86U44; TKKSRKH.
Other
NextBio 61981; -.
SOURCE METTL3; Homo sapiens.
ProtoNet Q86U44.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Direct protein sequencing; Methyltransferase; Nucleus; Phosphoprotein; RNA-binding; S-adenosyl-L-methionine; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   580  580     N6-adenosine-methyltransferase 70 kDa subunit. PRO_0000207630
MOD_RES   43    43        Phosphoserine. 
MOD_RES   219   219        Phosphoserine. 
MOD_RES   243   243        Phosphoserine. 
MOD_RES   350   350        Phosphoserine. 
MOD_RES   356   356        Phosphothreonine. 
VAR_SEQ   1   284        Missing (in isoform 2). VSP_007864
VAR_SEQ   486   505        GVKGNPQGFNQGLDCDVIVA -> SSSGAQFNRWSTKKNHLISY (in isoform 2). VSP_007865
VAR_SEQ   506   580        Missing (in isoform 2). VSP_007866
CONFLICT   251   251        N -> I (in Ref. 1; AAB71850). 
CONFLICT   263   264        KF -> I (in Ref. 1; AAB71850). 
CONFLICT   382   382        D -> V (in Ref. 1; AAB71850). 
CONFLICT   568   568        R -> K (in Ref. 4; AAH52244). 
Sequence information
Length: 580 AA [This is the length of the unprocessed precursor] Molecular weight: 64474 Da [This is the MW of the unprocessed precursor] CRC64: 63A7F10195A3C6AC [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSDTWSSIQA HKKQLDSLRE RLQRRRKQDS GHLDLRNPEA ALSPTFRSDS PVPTAPTSGG 

        70         80         90        100        110        120 
PKPSTASAVP ELATDPELEK KLLHHLSDLA LTLPTDAVSI CLAISTPDAP ATQDGVESLL 

       130        140        150        160        170        180 
QKFAAQELIE VKRGLLQDDA HPTLVTYADH SKLSAMMGAV AEKKGPGEVA GTVTGQKRRA 

       190        200        210        220        230        240 
EQDSTTVAAF ASSLVSGLNS SASEPAKEPA KKSRKHAASD VDLEIESLLN QQSTKEQQSK 

       250        260        270        280        290        300 
KVSQEILELL NTTTAKEQSI VEKFRSRGRA QVQEFCDYGT KEECMKASDA DRPCRKLHFR 

       310        320        330        340        350        360 
RIINKHTDES LGDCSFLNTC FHMDTCKYVH YEIDACMDSE APGSKDHTPS QELALTQSVG 

       370        380        390        400        410        420 
GDSSADRLFP PQWICCDIRY LDVSILGKFA VVMADPPWDI HMELPYGTLT DDEMRRLNIP 

       430        440        450        460        470        480 
VLQDDGFLFL WVTGRAMELG RECLNLWGYE RVDEIIWVKT NQLQRIIRTG RTGHWLNHGK 

       490        500        510        520        530        540 
EHCLVGVKGN PQGFNQGLDC DVIVAEVRST SHKPDEIYGM IERLSPGTRK IELFGRPHNV 

       550        560        570        580 
QPNWITLGNQ LDGIHLLDPD VVARFKQRYP DGIISKPKNL 

Q86U44 in FASTA format

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