ID   CTNA_DICDI              Reviewed;         258 AA.
AC   Q86IV5; Q556C3; Q9U7C4;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   22-JUL-2008, entry version 24.
DE   RecName: Full=Countin-1;
DE   Flags: Precursor;
GN   Name=ctnA; ORFNames=DDB_0185089;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Mycetozoa; Dictyosteliida; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-41, IDENTIFICATION
RP   IN THE COUNTIN COMPLEX, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION,
RP   FUNCTION, AND KNOCKOUT.
RX   MEDLINE=99375311; PubMed=10444594;
RA   Brock D.A., Gomer R.H.;
RT   "A cell-counting factor regulating structure size in Dictyostelium.";
RL   Genes Dev. 13:1960-1969(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   MEDLINE=22092622; PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A.,
RA   Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G.,
RA   Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A.,
RA   Platzer M., Rosenthal A., Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA   Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA   Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA   Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA   Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA   Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA   Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA   Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA   Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA   Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA   Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA   Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA   Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA   Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA   Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA   Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA   Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [4]
RP   FUNCTION.
RX   PubMed=11090633; DOI=10.1016/S1097-2765(00)00093-9;
RA   Roisin-Bouffay C., Jang W., Caprette D.R., Gomer R.H.;
RT   "A precise group size in Dictyostelium is generated by a cell-counting
RT   factor modulating cell-cell adhesion.";
RL   Mol. Cell 6:953-959(2000).
RN   [5]
RP   FUNCTION, AND DEVELOPMENTAL STAGE.
RX   MEDLINE=21600743; PubMed=11737154;
RX   DOI=10.1046/j.1440-169X.2001.00615.x;
RA   Okuwa T., Katayama T., Takano A., Kodaira K., Yasukawa H.;
RT   "Two cell-counting factors regulate the aggregate size of the cellular
RT   slime mold Dictyostelium discoideum.";
RL   Dev. Growth Differ. 43:735-744(2001).
CC   -!- FUNCTION: Cell-counting factor that limits the maximum size of the
CC       multicellular structure. May down-regulate the expression of gp24,
CC       which mediates cell adhesion.
CC   -!- SUBUNIT: Component of the counting factor (CF) complex, which
CC       includes cf60, cf50, cf45-1 and ctnA.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- DEVELOPMENTAL STAGE: Highly expressed in the vegetative cells and
CC       decreases during subsequent developmental stages (6-24 hours).
CC   -!- MISCELLANEOUS: Cells lacking cntA form a fewer number of larger
CC       aggregates.
CC   -!- SIMILARITY: Belongs to the countin family.
CC   -!- SIMILARITY: Contains 1 saposin B-type domain.
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DR   EMBL; AF140780; AAD53482.1; -; Genomic_DNA.
DR   EMBL; AC116986; AAO51901.1; -; Genomic_DNA.
DR   EMBL; AAFI02000012; EAL70191.1; -; Genomic_DNA.
DR   RefSeq; XP_643887.1; -.
DR   GeneID; 3395201; -.
DR   KEGG; ddi:DDB_0185089; -.
DR   dictyBase; DDB0185089; ctnA.
DR   InterPro; IPR008139; SaposinB.
DR   SMART; SM00741; SapB; 1.
DR   PROSITE; PS50015; SAP_B; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Direct protein sequencing; Glycoprotein; Secreted;
KW   Signal.
FT   SIGNAL        1     21
FT   CHAIN        22    258       Countin-1.
FT                                /FTId=PRO_0000327908.
FT   DOMAIN       22    106       Saposin B-type.
FT   CARBOHYD    121    121       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    215    215       N-linked (GlcNAc...) (Potential).
FT   DISULFID     25    102       By similarity.
FT   DISULFID     28     96       By similarity.
FT   DISULFID     56     69       By similarity.
FT   CONFLICT     45     45       Missing (in Ref. 1; AAD53482).
SQ   SEQUENCE   258 AA;  26659 MW;  725F37870E78356A CRC64;
     MNKLFSLILA LFLVNSAVVS SLDSCSICVD FVGNSLNDLL NIILNSGVIG TCGDLCSAVP
     GGQIVDTVCD LLCDYVGVDE FIKLISDVDP DPIYICEKIS VCKTNDNAAA SLDLVQINPQ
     NGTVGGTFTL SIAYTVTNTI ATGQLAFNII DPTGNAFGDA VLLVQQSPEQ YTQQFQFQAT
     PSEQESFPNG LYTVQALVCE GSCGSPHPNT YTLANGTTTF TISGADSSSM SGAGSFSGSS
     QSTQTGAASG SGSGFALF
//