RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Infectious hematopoietic necrosis virus (strain Oregon69) (IHNV)

[TaxID: 429315]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; Novirhabdovirus.

Virus host

Salmo [TaxID: 8028]

Protein existence

3: Inferred from homology;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. PubMed=8683214 [NCBI, ExPASy, EBI, Israel, Japan] Schutze H., Enzmann P.J., Mundt E., Mettenleiter T.C.; "Identification of the non-virion (NV) protein of fish rhabdoviruses viral haemorrhagic septicaemia virus and infectious haematopoietic necrosis virus."; J. Gen. Virol. 77:1259-1263(1996).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. PubMed=7595355 [NCBI, ExPASy, EBI, Israel, Japan] Schutze H., Enzmann P.J., Kuchling R., Mundt E., Niemann H., Mettenleiter T.C.; "Complete genomic sequence of the fish rhabdovirus infectious haematopoietic necrosis virus."; J. Gen. Virol. 76:2519-2527(1995).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently six subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
SIMILARITY: Belongs to the rhabdoviridae protein L family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X89213; CAA61500.1; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q82707.

Family and domain databases

InterPro

IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Complete proteome; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1986`	`1986`	`Large structural protein.`	`PRO_0000282897`
`DOMAIN`	`560 757`	`198`	`RdRp catalytic.`
`NP_BIND`	`1619 1628`	`10`	`ATP (Potential).`

Sequence information

Length: 1986 AA [This is the length of the unprocessed precursor]

Molecular weight: 225021 Da [This is the MW of the unprocessed precursor]

CRC64: 25FE029E810846F9 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDFFDLDIEI KQERLPAECS LNSPLNYSLS AQLTDRMTPR TENVRRQRER LRSHMREHFR 

        70         80         90        100        110        120 
VKDLSTLDND STRLHARLTE DLITIQSPEI DSSVLENWTP LKSYYASLDY TLPEKTAFKW 

       130        140        150        160        170        180 
EHAAPYWNLF AQLRAILLQS QKIKKQETGT RELYSCGPLQ IEFVEGVVLY FTDRGSKEEF 

       190        200        210        220        230        240 
TKSGELPSVT PYADFLAWIK IISQRAQAVL MAVILRVTDK GLSPLPESLL AIYQNVDDIL 

       250        260        270        280        290        300 
KRAGQPAIDL LKLWEPLVIT KLGELLGDRF GLEEDFRNTI RGEATKLAKN LFISRGLNRL 

       310        320        330        340        350        360 
MDILDQQTDA QPLFQFFGLF KHFAYPRVFS RDTIQAIQEV SDRPSSISAV DFLHDQCEIR 

       370        380        390        400        410        420 
KEFYVRYINA YHRAPGLDLS ALSPSSFLRD SLERGKIPNE RSPLYSNKEW YFVKFTKSIE 

       430        440        450        460        470        480 
WPVSDTLSTF LSDKAITRDR PAWIEDGHSG RDMSEKRLLL KFIKENFSSV ADIVGAAEAI 

       490        500        510        520        530        540 
YNNQEDRLIA LKVKEMELKI KGRGFGLMTF MPRLLQVLRE SIAKKTQKLF PEITMTSSDL 

       550        560        570        580        590        600 
DMKKRKFMLS KKSDDRRGFI HVNKSLDINK FCTSQRQFNS NAVFSSLDEL MGTFPLFSRV 

       610        620        630        640        650        660 
HEIFEKTWIV DGSSSDPPNL AHFTRILDEC TALGLDTPHI WADGVFSGLK GGIEGLCQYV 

       670        680        690        700        710        720 
WTICLLLRVE RVMQKTALTH YILAQGDNVI ITIIVPVEIH RDGTIPEQES RRILALSRDI 

       730        740        750        760        770        780 
DLSLESELEK SGLTLKIEET LTSENISIYG KDLHCPQHLT LAIKKAASAA IISSEQYQDV 

       790        800        810        820        830        840 
PTFLSGLGTS LEALSECVNN KVGVHLFGVI MGVAGWRDLA THQTWRGWRY PYHKKAITGR 

       850        860        870        880        890        900 
IRASEMKLSK GEPTELSISV LSKRRRETET LIELLSNSLL GSALGMLAFP TPLDLEKRGV 

       910        920        930        940        950        960 
GDYITHRLTI ARKALLSGHL DPRIGRKVES ACNIPLSSRT DLSKLFDSPF SLNIATEEDA 

       970        980        990       1000       1010       1020 
TAVIKRQATK ILRLQEIKNE KLKAQIDNMD KGIATLDAAL AGATNINPRL NYMIRSITDE 

      1030       1040       1050       1060       1070       1080 
KESEMFVTKF ASARTMRTLA MNHSSELPIV ILLEMKSQQK ETYTIWRTKR PPVTMWKCST 

      1090       1100       1110       1120       1130       1140 
VLAKELRDTS WGKNIIGGTS PSPIEAMETI QIDPTEWEDR RSQDAMSINY YLSRAGMDEQ 

      1150       1160       1170       1180       1190       1200 
TAKLTRGFLV PYYGTQTKPL VAKAYLELKG NPRTNKALLL LSVRESLVKT GSNLDKLIIK 

      1210       1220       1230       1240       1250       1260 
LCSHALDIDV ASLPALRAQE EAAAGEGLRG GIKESMSPVG PDNFYTNITH KVFNRKWATP 

      1270       1280       1290       1300       1310       1320 
YHVNIADFII QGLIETRRHL LVNERMNGLL PVSSVKCTSC FRKKEREFFD IPEEFTWKNE 

      1330       1340       1350       1360       1370       1380 
SKTSDPAYTY FTTWCDLPRV SNLPEMDQRS ATRLLGRGLA LNRSSSGEII TKFYSMPMES 

      1390       1400       1410       1420       1430       1440 
QRLLHPVELL LGYGEGVIFG YLRSQHINHG ALFHIGDESL AKKLRRYVLD TKTQHAKQIG 

      1450       1460       1470       1480       1490       1500 
YLFQDEDSLH ELLGQGLCPY IPRSIPLTIT ELTNACAITT IRATEVILST KARIHHMPVQ 

      1510       1520       1530       1540       1550       1560 
AIDESDVDTS RLAANTMQTI LGDPRPMNLV HLDCDLTHNM VAWESEVELD ILKSENFHID 

      1570       1580       1590       1600       1610       1620 
GLLVELTARE LPIGDTPWKQ RDWTCSNDPK IIAKGIKTKS LFIHQGVTGA INLIPDLLVV 

      1630       1640       1650       1660       1670       1680 
IGGGLGGCAV PYLQEWPDTP IIFATLFDER ERISEDGDLI VPPELLVRGM APRMIEREIL 

      1690       1700       1710       1720       1730       1740 
EAELCDITNE GNRRLLIRLV TKNKGGGKVV LIDEIENRGA PESLLQSSLQ DLFEKLDKVC 

      1750       1760       1770       1780       1790       1800 
KLTSVHTVRE STVEQFSQRV NSIKRSRKAV TLHWNRYNRR DQFEALVIVK GEEAKSDYRI 

      1810       1820       1830       1840       1850       1860 
STITSAKAFR KIDEQLEIDG RLSSTQWSLP ALPSREKNIL FGYVSSVFLK MNLALSANDM 

      1870       1880       1890       1900       1910       1920 
DRERLIETIE GTAPGLISWK EKLEHRDHAH RSDIEEKGIT QDKIFNLICL SWVLKGLRYG 

      1930       1940       1950       1960       1970       1980 
VWDTDAQSIV AQTVYITRGP KLCPLGEKPK RIFASFKLQS GKRVEDAKGF LSALLHLEGF 


FPLGEQ

Q82707 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools