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UniProtKB/Swiss-Prot entry Q81T67


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LEU3_BACAN
Primary accession number Q81T67
Secondary accession numbers Q6I1F0 Q6KV96
Integrated into Swiss-Prot on November 14, 2003
Sequence was last modified on June 1, 2003 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 42)
Name and origin of the protein
Protein name 3-isopropylmalate dehydrogenase
Synonyms EC 1.1.1.85
Beta-IPM dehydrogenase
IMDH
3-IPM-DH
Gene name
Name: leuB
OrderedLocusNames: BA_1421, GBAA1421, BAS1312
From
Bacillus anthracis [TaxID: 1392] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus; Bacillus cereus group.
Protein existence 3: Inferred from homology;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Ames / isolate Porton;
DOI=10.1038/nature01586; PubMed=12721629 [NCBI, ExPASy, EBI, Israel, Japan]
Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J., Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.;
"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria.";
Nature 423:81-86(2003).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Ames ancestor;
Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M.;
"Bacillus anthracis comparative genomics.";
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Sterne;
Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.;
"Complete genome sequence of Bacillus anthracis Sterne.";
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE016879; AAP25364.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE017334; AAT30517.2; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE017225; AAT53632.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_843878.1; -.
YP_018042.2; -.
YP_027581.1; -.
3D structure databases
HSSP P12010; 2AYQ. [HSSP ENTRY / PDB]
ModBase Q81T67.
Enzyme and pathway databases
BioCyc BANT260799:BAS1312-MON; -.
BANT261594:GBAA1421-MON; -.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0003862; Molecular function: 3-isopropylmalate dehydrogenase activity (inferred from electronic annotation from HAMAP).
GO:0009098; Biological process: leucine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.
Family and domain databases
HAMAP MF_01033; -; 1.
PBIL [Tree]
InterPro IPR004429; 3-isopropylmalate_DHase.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.718.10; IDH_IMDH; 1.
PANTHER PTHR11835; IDH_IMDH_dimeric; 1.
PTHR11835:SF13; IPMDH; 1.
Pfam PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00169; leuB; 1.
PROSITE PS00470; IDH_IMDH; 1.
BLOCKS Q81T67.
Genome annotation databases
GeneID 1084163; -.
2816381; -.
2848026; -.
GenomeReviews AE016879_GR; BA_1421.
AE017225_GR; BAS1312.
AE017334_GR; GBAA1421.
KEGG ban:BA1421; -.
bar:GBAA1421; -.
bat:BAS1312; -.
TIGR BA_1421; -.
GBAA1421; -.
Phylogenomic databases
HOGENOM Q81T67; -.
Other
ProtoNet Q81T67.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Cytoplasm; Leucine biosynthesis; Magnesium; Manganese; Metal-binding; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   354  354     3-isopropylmalate dehydrogenase. PRO_0000083632
NP_BIND   76    87  12     NAD (By similarity). 
NP_BIND   273   285  13     NAD (By similarity). 
METAL   215   215        Magnesium or manganese (By similarity). 
METAL   239   239        Magnesium or manganese (By similarity). 
METAL   243   243        Magnesium or manganese (By similarity). 
BINDING   94    94        Substrate (By similarity). 
BINDING   104   104        Substrate (By similarity). 
BINDING   130   130        Substrate (By similarity). 
BINDING   215   215        Substrate (By similarity). 
SITE   137   137  1     Important for catalysis (By similarity). 
SITE   183   183  1     Important for catalysis (By similarity). 
Sequence information
Length: 354 AA [This is the length of the unprocessed precursor] Molecular weight: 38329 Da [This is the MW of the unprocessed precursor] CRC64: 01D112EABD49E9E9 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MEKRIVCLAG DGVGPEVMES AKEVLHMVER LYGHHFHLQD EHFGGVAIDL TGQPLPQRTL 

        70         80         90        100        110        120 
AACLASDAVL LGAVGGPRWD GAKERPEKGL LALRKGLGVF ANVRPVTVES ATAHLSPLKK 

       130        140        150        160        170        180 
ADEIDFVVVR ELTGGIYFSY PKERTDEVAT DTLTYHRHEI ERIVSCAFQL ASKRKKKVTS 

       190        200        210        220        230        240 
IDKANVLESS KLWRIVTEEV ALRYPDVELE HILVDAAAME LIRNPGRFDV IVTENLFGDI 

       250        260        270        280        290        300 
LSDEASVLAG SLGMLPSASH AEKGPSLYEP IHGSAPDIAG KNKANPIAMM RSVAMMLGQS 

       310        320        330        340        350 
FGLTREGCAI EEAISAVLKS GKCTADIGGT ETTTSFTKAV MQEMEEQALV GRGR 

Q81T67 in FASTA format

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