Protein p19
Transmembrane protein 1A
Serine protease p27
     (p27)
     (EC 3.4.21.-)
Protein p20
RNA-directed RNA polymerase p57
     (p57)
     (EC 2.7.7.48)

Gene name

Name:

ORF1

From

Mink astrovirus 1 (MAstV-1)

[TaxID: 219688]

Taxonomy

Viruses; ssRNA positive-strand viruses, no DNA stage; Astroviridae; Mamastrovirus.

Virus host

Mustela vison (American mink) (Neovison vison) [TaxID: 452646]

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1099/vir.0.19267-0; PubMed=14573813 [NCBI, ExPASy, EBI, Israel, Japan]
Mittelholzer C., Hedlund K.O., Englund L., Dietz H.H., Svensson L.;
"Molecular characterization of a novel astrovirus associated with disease in mink.";
J. Gen. Virol. 84:3087-3094(2003).

Comments

FUNCTION: Non-structural polyprotein 1AB contains the viral protease participating in the cleavage of the polyprotein into functional products. It contains also the activities necessary for replication of genomic RNA, as well as transcription of subgenomic mRNA (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by ribosomal frameshifting.

Name	nsp1ab
Isoform ID	Q80KJ7-1
Note: Generated by a ribosomal frameshift at position 865.
This is the isoform sequence displayed in this entry.

Name	nsp1a
Isoform ID	Q80KJ8-1
This isoform is stored in UniProtKB/Swiss-Prot entry Q80KJ8.

PTM: Cleaved presumably by viral and host proteases (By similarity).
SIMILARITY: Belongs to the astroviridae polyprotein 1AB family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AY179509; AAO32082.1; ALT_SEQ; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q80KJ7.

Family and domain databases

InterPro

IPR003439; ABC_transporter-like.
IPR001730; Peptidase_C4.
IPR001254; Peptidase_S1_S6.
IPR007094; RNA_pol_PSvir.
Graphical view of domain structure.

Pfam

PF00863; Peptidase_C4; 1.
Pfam graphical view of domain structure.

PROSITE

PS50507; RDRP_SSRNA_POS; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Coiled coil; Hydrolase; Membrane; Nucleotide-binding; Nucleotidyltransferase; Protease; Ribosomal frameshifting; RNA replication; RNA-directed RNA polymerase; Thiol protease; Transferase; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1381`	`1381`	`Non-structural polyprotein 1AB.`	`PRO_0000327295`
`CHAIN`	`1 195`	`195`	`Protein p19 (Potential).`	`PRO_0000327296`
`CHAIN`	`196 436`	`241`	`Transmembrane protein 1A (Potential).`	`PRO_0000327297`
`CHAIN`	`437 662`	`226`	`Serine protease p27 (Potential).`	`PRO_0000327298`
`CHAIN`	`663 867`	`205`	`Protein p20 (Potential).`	`PRO_0000327299`
`CHAIN`	`868 1381`	`514`	`RNA-directed RNA polymerase p57 (Potential).`	`PRO_0000327300`
`TRANSMEM`	`169 189`	`21`	`Potential.`
`TRANSMEM`	`249 269`	`21`	`Potential.`
`TRANSMEM`	`279 299`	`21`	`Potential.`
`TRANSMEM`	`324 344`	`21`	`Potential.`
`TRANSMEM`	`365 385`	`21`	`Potential.`
`DOMAIN`	`1122 1254`	`133`	`RdRp catalytic.`
`COILED`	`121 160`	`40`	`Potential.`
`COILED`	`703 732`	`30`	`Potential.`
`ACT_SITE`	`477 477`		`Charge relay system; for serine protease activity (By similarity).`
`ACT_SITE`	`506 506`		`Charge relay system; for serine protease activity (By similarity).`
`ACT_SITE`	`569 569`		`Charge relay system; for serine protease activity (By similarity).`
`SITE`	`195 196`	`2`	`Cleavage (Potential).`
`SITE`	`436 437`	`2`	`Cleavage (Potential).`
`SITE`	`662 663`	`2`	`Cleavage (Potential).`
`SITE`	`867 868`	`2`	`Cleavage (Potential).`

Sequence information

Length: 1381 AA [This is the length of the unprocessed precursor]

Molecular weight: 156954 Da [This is the MW of the unprocessed precursor]

CRC64: 51B926928D22E51C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MANNTTSALH PRGSGQRCVY DTVLRFGDPD ARRRGFQLDE VSHNKLCDIF DSGPLHFAFG 

        70         80         90        100        110        120 
DLKVMKVAGG VVTPHKTVVK TVYVSGVQEG NDYVTFAFTP GPNEWREVDP RIDKRTALVG 

       130        140        150        160        170        180 
VLVQEHKKLD SDLKESRREL SQLKLEHSLL RHDYERLVRE KPGPAMRTFK FSAVIFYAFF 

       190        200        210        220        230        240 
LGFLLMSAVK GEVYGRCLDS ELNLNGNPEV CLHWEEVKSF SLQVALADFW NMTLDYYATV 

       250        260        270        280        290        300 
APQSPLMDLA LGYFPYFANW HMAAFLVGTA HVVAAERPLY MLVTLVLATL SRFQLVALAA 

       310        320        330        340        350        360 
VPMLDMPSSI GLWVTMVLFA IDQAFAILAS VLISVLLLIL CLAMNDVDYG ALLRGCVTLV 

       370        380        390        400        410        420 
SATVFSHLVS FLHAPGWFTI IAILIYRIPK VLSYVSAERV DIKGPDGKIK ETQNANPSWI 

       430        440        450        460        470        480 
TKMSGLKNFF QRAFRQKVRT GVNPTTRIIP NSLVVIDAKD GRGTGFRVRN YLVTAGHVVG 

       490        500        510        520        530        540 
ADTTVRVRWA DVTSFAHVVY RVPNKDIVLL TLPAEYNSLH SYKLAKEVVD GTVVVVSNGD 

       550        560        570        580        590        600 
GGALSVGISE GVIVGESMTY AINTADGMSG SPLTTTDGRL IGVHQQNTGF TGGAVIFRDT 

       610        620        630        640        650        660 
DFPQPKKPQR EADLEAKVAE LEKALAAYTQ SATGEDIVGL VRVAIQREME VLRKELSNEF 

       670        680        690        700        710        720 
GQAKGKTKHK RRIMAAARSG GKRKPGKVWT EEEYKKLLEE GFTRDQLREM AEAAREADDD 

       730        740        750        760        770        780 
FDDYEEEKNE VDYPVWSDHD SDEEIDRDWF GQNLPTWSSA WSDFEPELDP DVTKTLPCHL 

       790        800        810        820        830        840 
EDKFSLKHYI ITEADLKHFG QEMKEYMDHL DAVIKTHTEK GKWCPNTNTE EILKDLNAMW 

       850        860        870        880        890        900 
FKLNHTMWKN GVAPFMQRKK QKPKKREEGP ERGPINPDEM RLDHWEKMMA PPDAGRRLVP 

       910        920        930        940        950        960 
DDYPVIGKLP INRPISDWDE PVDDLLNLLP PAPDSSAYGP AVWGPEAYVK SFEKFTYAKP 

       970        980        990       1000       1010       1020 
RDSIKRDFPR EWKFACQVLR REFDFLEGSV IMDITATSKN ADSTCAYPKC NYWKTEAEYL 

      1030       1040       1050       1060       1070       1080 
SERGYQDYVS EYKRIHGGAR PKVLWLLFLK KEILKVKKIN DSDIRQIVCA DPIFARIGNA 

      1090       1100       1110       1120       1130       1140 
FEEHQNTLMK HRTATRMPQC GWTPFFNGFK RRIERLLSRK NSVFIEFDWT RYDGTIPREI 

      1150       1160       1170       1180       1190       1200 
FAKIKSFRFS CLAEEFQTDA NRAMYQWYCD SLLDRYVLMP SGEVTRQTKG NPSGQISTTM 

      1210       1220       1230       1240       1250       1260 
DNNLCNVFFQ AFEYAYIHPE KSIEELRESW DRCDSLIYGD DRLTTFDHVP PDYVDRVVHM 

      1270       1280       1290       1300       1310       1320 
YKDVFGMWVK PEKVIVSDTP VGLSFCGFTV GPDLMPVPTD CDKLVASLVT PTKKLQDIVA 

      1330       1340       1350       1360       1370       1380 
LYSKVLCYRI LGHNLSDEHE FKRYVRVALE VLARHIRNLG GEEPVHVTER LLDKLWRGGP 


K

Q80KJ7 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools